Phenotyping tree resistance to a bark chewing insect, the pine weevil Hylobius abietis

Breeding for resistance to forest pests and pathogens is emerging as a promising tool for minimising the impact of the increasing biotic threats that our forests are experiencing as a consequence of global change. Efficient phenotyping protocols of resistance are urgently needed. Here we present the...

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Autores: Zas Arregui, Rafael, Poceiro, María Silvana, Lores Méndez, María, Rolke, Laura M., Sampedro Pérez, Luis
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/186555
Acceso en línea:http://hdl.handle.net/10261/186555
Access Level:acceso abierto
Palabra clave:Breeding for resistance
Induced defences
Forest pests
Mediterranean pines
Nonvolatile resin
Phenotyping protocols
Pinus pinaster
Pinus pinea
Pinus radiata
Pinus sylvestris
Screening
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dc.title.none.fl_str_mv Phenotyping tree resistance to a bark chewing insect, the pine weevil Hylobius abietis
title Phenotyping tree resistance to a bark chewing insect, the pine weevil Hylobius abietis
spellingShingle Phenotyping tree resistance to a bark chewing insect, the pine weevil Hylobius abietis
Zas Arregui, Rafael
Breeding for resistance
Induced defences
Forest pests
Mediterranean pines
Nonvolatile resin
Phenotyping protocols
Pinus pinaster
Pinus pinea
Pinus radiata
Pinus sylvestris
Screening
title_short Phenotyping tree resistance to a bark chewing insect, the pine weevil Hylobius abietis
title_full Phenotyping tree resistance to a bark chewing insect, the pine weevil Hylobius abietis
title_fullStr Phenotyping tree resistance to a bark chewing insect, the pine weevil Hylobius abietis
title_full_unstemmed Phenotyping tree resistance to a bark chewing insect, the pine weevil Hylobius abietis
title_sort Phenotyping tree resistance to a bark chewing insect, the pine weevil Hylobius abietis
dc.creator.none.fl_str_mv Zas Arregui, Rafael
Poceiro, María Silvana
Lores Méndez, María
Rolke, Laura M.
Sampedro Pérez, Luis
author Zas Arregui, Rafael
author_facet Zas Arregui, Rafael
Poceiro, María Silvana
Lores Méndez, María
Rolke, Laura M.
Sampedro Pérez, Luis
author_role author
author2 Poceiro, María Silvana
Lores Méndez, María
Rolke, Laura M.
Sampedro Pérez, Luis
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
0000-0002-3921-2575
0000-0001-6563-2461
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Breeding for resistance
Induced defences
Forest pests
Mediterranean pines
Nonvolatile resin
Phenotyping protocols
Pinus pinaster
Pinus pinea
Pinus radiata
Pinus sylvestris
Screening
topic Breeding for resistance
Induced defences
Forest pests
Mediterranean pines
Nonvolatile resin
Phenotyping protocols
Pinus pinaster
Pinus pinea
Pinus radiata
Pinus sylvestris
Screening
description Breeding for resistance to forest pests and pathogens is emerging as a promising tool for minimising the impact of the increasing biotic threats that our forests are experiencing as a consequence of global change. Efficient phenotyping protocols of resistance are urgently needed. Here we present the results of two experiments aimed to determine whether the variation in resistance to the pine weevil Hylobius abietis, a harmful pest of European conifers, can be inferred by nondestructive bioassays using excised plant material collected in forest genetic trials. Weevil damage and amount of nonvolatile resin induced by weevil feeding were assessed in young trees and in branches of adult trees using several phenotyping procedures (bioassays using either living trees, excised plant material and cut stem twigs) on four pine species (Pinus pinaster, P. radiata, P. sylvestris and P. pinea). Half of the plants were previously induced with methyl jasmonate (MJ), a treatment that is known to affect resistance to the pine weevil. In Experiment 1, living and excised plants showed parallel results: MJ treatment significantly reduced weevil damage, and saplings responded to weevil damage locally increasing the nonvolatile resin (NVR) in the stems proportionally to the damage suffered. This response was, however, slightly lower in excised than in living saplings. On the contrary, patterns of weevil feeding on stem twigs completely departed from those observed in living and excised seedlings. Moreover, cut stem twigs were unable to respond to weevil feeding increasing NVR according to the weevil damage. In Experiment 2, assessment of weevil damage on excised branches explained around 50% of variation in damage on living branches. This relationship became much more pronounced (R2 = 0.81) when explored at the mean treatment level; branch manipulation did not alter the patterns of variation in resistance across pine species or MJ treatments. Irrespective of the assessment procedure, MJ consistently decreased weevil damage in all pine species, with larger reduction in weevil damage in stone and maritime pine than in radiata and Scots pine. Radiata pine was the most resistant while Scots pine was the most susceptible to the pine weevil. Overall, results suggest that using excised plant material is an operative alternative for phenotyping weevil resistance whenever care is taken to maintain the functionality of the excised plant material. This will allow taking advantage of multiple available conifer genetic trials to deepen the ecological genetics of resistance to the pine weevil and to screen for resistance without compromising the long‐term utility of those genetic trials.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/186555
url http://hdl.handle.net/10261/186555
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-68274-C3-2-R
info:eu-repo/grantAgreement/EC/H2020/773383
http://dx.doi.org/10.1111/aab.12533
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Phenotyping tree resistance to a bark chewing insect, the pine weevil Hylobius abietisZas Arregui, RafaelPoceiro, María SilvanaLores Méndez, MaríaRolke, Laura M.Sampedro Pérez, LuisBreeding for resistanceInduced defencesForest pestsMediterranean pinesNonvolatile resinPhenotyping protocolsPinus pinasterPinus pineaPinus radiataPinus sylvestrisScreeningBreeding for resistance to forest pests and pathogens is emerging as a promising tool for minimising the impact of the increasing biotic threats that our forests are experiencing as a consequence of global change. Efficient phenotyping protocols of resistance are urgently needed. Here we present the results of two experiments aimed to determine whether the variation in resistance to the pine weevil Hylobius abietis, a harmful pest of European conifers, can be inferred by nondestructive bioassays using excised plant material collected in forest genetic trials. Weevil damage and amount of nonvolatile resin induced by weevil feeding were assessed in young trees and in branches of adult trees using several phenotyping procedures (bioassays using either living trees, excised plant material and cut stem twigs) on four pine species (Pinus pinaster, P. radiata, P. sylvestris and P. pinea). Half of the plants were previously induced with methyl jasmonate (MJ), a treatment that is known to affect resistance to the pine weevil. In Experiment 1, living and excised plants showed parallel results: MJ treatment significantly reduced weevil damage, and saplings responded to weevil damage locally increasing the nonvolatile resin (NVR) in the stems proportionally to the damage suffered. This response was, however, slightly lower in excised than in living saplings. On the contrary, patterns of weevil feeding on stem twigs completely departed from those observed in living and excised seedlings. Moreover, cut stem twigs were unable to respond to weevil feeding increasing NVR according to the weevil damage. In Experiment 2, assessment of weevil damage on excised branches explained around 50% of variation in damage on living branches. This relationship became much more pronounced (R2 = 0.81) when explored at the mean treatment level; branch manipulation did not alter the patterns of variation in resistance across pine species or MJ treatments. Irrespective of the assessment procedure, MJ consistently decreased weevil damage in all pine species, with larger reduction in weevil damage in stone and maritime pine than in radiata and Scots pine. Radiata pine was the most resistant while Scots pine was the most susceptible to the pine weevil. Overall, results suggest that using excised plant material is an operative alternative for phenotyping weevil resistance whenever care is taken to maintain the functionality of the excised plant material. This will allow taking advantage of multiple available conifer genetic trials to deepen the ecological genetics of resistance to the pine weevil and to screen for resistance without compromising the long‐term utility of those genetic trials.This research has received funding from the Spanish Government via the MINECO/FEDER grant FUTURPIN (AGL2015-68274-C03-02-R) and from the European Union's Horizon 2020 Program for Research and Innovation under grant agreement nº 773383.Peer reviewedJohn Wiley & SonsMinisterio de Economía y Competitividad (España)European Commission0000-0002-3921-25750000-0001-6563-2461Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/186555reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-68274-C3-2-Rinfo:eu-repo/grantAgreement/EC/H2020/773383http://dx.doi.org/10.1111/aab.12533Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1865552026-05-22T06:33:51Z
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