Comprehensive structure and functional adaptations of the yeast nuclear pore complex

Nuclear pore complexes (NPCs) mediate the nucleocytoplasmic transport of macromolecules. Here we provide a structure of the isolated yeast NPC in which the inner ring is resolved by cryo-EM at sub-nanometer resolution to show how flexible connectors tie together different structural and functional l...

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Detalhes bibliográficos
Autores: Akey, Christopher W., Singh, Digvijay, Ouch, Christna, Echeverría, Ignacia, Nudelman, Ilona, Varberg, Joseph M., Yu, Zulin, Fang, Fei, Shi, Yi, Wang, Junjie, Salzberg, Daniel, Song, Kangkang, Xu, Chen, Gumbart, James C., Suslov, Sergey, Unruh, Jay, Jaspersen, Sue, Chait, Brian T., Sali, Andrej, Fernández-Martínez, Javier, Ludtke, Steven J., Villa, Elizabeth, Rout, Michael P.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/304163
Acesso em linha:http://hdl.handle.net/10261/304163
https://api.elsevier.com/content/abstract/scopus_id/85122983841
Access Level:acceso abierto
Palavra-chave:NPC evolution
Nuclear pore complex
Cryo-electron microscopy
Cryo-electron tomography
Inner ring dilation
Nuclear basket
Nucleocytoplasmic transport
Nucleoporins
Structural isoforms
Descrição
Resumo:Nuclear pore complexes (NPCs) mediate the nucleocytoplasmic transport of macromolecules. Here we provide a structure of the isolated yeast NPC in which the inner ring is resolved by cryo-EM at sub-nanometer resolution to show how flexible connectors tie together different structural and functional layers. These connectors may be targets for phosphorylation and regulated disassembly in cells with an open mitosis. Moreover, some nucleoporin pairs and transport factors have similar interaction motifs, which suggests an evolutionary and mechanistic link between assembly and transport. We provide evidence for three major NPC variants that may foreshadow functional specializations at the nuclear periphery. Cryo-electron tomography extended these studies, providing a model of the in situ NPC with a radially expanded inner ring. Our comprehensive model reveals features of the nuclear basket and central transporter, suggests a role for the lumenal Pom152 ring in restricting dilation, and highlights structural plasticity that may be required for transport.

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