Multiocular organoids from human induced pluripotent stem cells displayed retinal, corneal, and retinal pigment epithelium lineages

Cornea; Ocular organoids; Ocular precursor cells

Detalles Bibliográficos
Autores: Isla Magrané, Helena, Veiga, Anna, García Arumí, José, Duarri Piqué, Anna
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Departament de Salut de la Generalitat de Catalunya (DS)
Repositorio:Scientia. Dipòsit d'Informació Digital del Departament de Salut
OAI Identifier:oai:scientiasalut.gencat.cat:11351/7565
Acceso en línea:https://hdl.handle.net/11351/7565
Access Level:acceso abierto
Palabra clave:Diferenciació cel·lular
Ulls - Malalties
Medicina regenerativa
ANATOMY::Sense Organs::Eye::Pigment Epithelium of Eye::Retinal Pigment Epithelium
ANATOMY::Cells::Stem Cells::Adult Stem Cells::Induced Pluripotent Stem Cells
PHENOMENA AND PROCESSES::Cell Physiological Phenomena::Cell Differentiation
ANATOMÍA::órganos de los sentidos::ojo::epitelio pigmentado ocular::epitelio pigmentado de la retina
ANATOMÍA::células::células madre::células madre adultas::células madre pluripotentes inducidas
FENÓMENOS Y PROCESOS::fenómenos fisiológicos celulares::diferenciación celular
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network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Multiocular organoids from human induced pluripotent stem cells displayed retinal, corneal, and retinal pigment epithelium lineages
title Multiocular organoids from human induced pluripotent stem cells displayed retinal, corneal, and retinal pigment epithelium lineages
spellingShingle Multiocular organoids from human induced pluripotent stem cells displayed retinal, corneal, and retinal pigment epithelium lineages
Isla Magrané, Helena
Diferenciació cel·lular
Ulls - Malalties
Medicina regenerativa
ANATOMY::Sense Organs::Eye::Pigment Epithelium of Eye::Retinal Pigment Epithelium
ANATOMY::Cells::Stem Cells::Adult Stem Cells::Induced Pluripotent Stem Cells
PHENOMENA AND PROCESSES::Cell Physiological Phenomena::Cell Differentiation
ANATOMÍA::órganos de los sentidos::ojo::epitelio pigmentado ocular::epitelio pigmentado de la retina
ANATOMÍA::células::células madre::células madre adultas::células madre pluripotentes inducidas
FENÓMENOS Y PROCESOS::fenómenos fisiológicos celulares::diferenciación celular
title_short Multiocular organoids from human induced pluripotent stem cells displayed retinal, corneal, and retinal pigment epithelium lineages
title_full Multiocular organoids from human induced pluripotent stem cells displayed retinal, corneal, and retinal pigment epithelium lineages
title_fullStr Multiocular organoids from human induced pluripotent stem cells displayed retinal, corneal, and retinal pigment epithelium lineages
title_full_unstemmed Multiocular organoids from human induced pluripotent stem cells displayed retinal, corneal, and retinal pigment epithelium lineages
title_sort Multiocular organoids from human induced pluripotent stem cells displayed retinal, corneal, and retinal pigment epithelium lineages
dc.creator.none.fl_str_mv Isla Magrané, Helena
Veiga, Anna
García Arumí, José
Duarri Piqué, Anna
author Isla Magrané, Helena
author_facet Isla Magrané, Helena
Veiga, Anna
García Arumí, José
Duarri Piqué, Anna
author_role author
author2 Veiga, Anna
García Arumí, José
Duarri Piqué, Anna
author2_role author
author
author
dc.contributor.none.fl_str_mv Institut Català de la Salut
[Isla-Magrané H, Duarri A] Grup de Recerca en Oftalmologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. [Veiga A] Regenerative Medicine Program IDIBELL, L’Hospitalet de Llobregat, Barcelona, Spain. [García-Arumí J] Grup de Recerca en Oftalmologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei d’Oftalmologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Departament d’Oftalmologia, Universitat Autònoma de Barcelona, Bellaterra, Spain
Vall d'Hebron Barcelona Hospital Campus
dc.subject.none.fl_str_mv Diferenciació cel·lular
Ulls - Malalties
Medicina regenerativa
ANATOMY::Sense Organs::Eye::Pigment Epithelium of Eye::Retinal Pigment Epithelium
ANATOMY::Cells::Stem Cells::Adult Stem Cells::Induced Pluripotent Stem Cells
PHENOMENA AND PROCESSES::Cell Physiological Phenomena::Cell Differentiation
ANATOMÍA::órganos de los sentidos::ojo::epitelio pigmentado ocular::epitelio pigmentado de la retina
ANATOMÍA::células::células madre::células madre adultas::células madre pluripotentes inducidas
FENÓMENOS Y PROCESOS::fenómenos fisiológicos celulares::diferenciación celular
topic Diferenciació cel·lular
Ulls - Malalties
Medicina regenerativa
ANATOMY::Sense Organs::Eye::Pigment Epithelium of Eye::Retinal Pigment Epithelium
ANATOMY::Cells::Stem Cells::Adult Stem Cells::Induced Pluripotent Stem Cells
PHENOMENA AND PROCESSES::Cell Physiological Phenomena::Cell Differentiation
ANATOMÍA::órganos de los sentidos::ojo::epitelio pigmentado ocular::epitelio pigmentado de la retina
ANATOMÍA::células::células madre::células madre adultas::células madre pluripotentes inducidas
FENÓMENOS Y PROCESOS::fenómenos fisiológicos celulares::diferenciación celular
description Cornea; Ocular organoids; Ocular precursor cells
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11351/7565
url https://hdl.handle.net/11351/7565
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Stem Cell Research & Therapy;12
https://doi.org/10.1186/s13287-021-02651-9
info:eu-repo/grantAgreement/ES/PE2017-2020/AC19%2F00080
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv Scientia
reponame:Scientia. Dipòsit d'Informació Digital del Departament de Salut
instname:Departament de Salut de la Generalitat de Catalunya (DS)
instname_str Departament de Salut de la Generalitat de Catalunya (DS)
reponame_str Scientia. Dipòsit d'Informació Digital del Departament de Salut
collection Scientia. Dipòsit d'Informació Digital del Departament de Salut
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405592874909696
spelling Multiocular organoids from human induced pluripotent stem cells displayed retinal, corneal, and retinal pigment epithelium lineagesIsla Magrané, HelenaVeiga, AnnaGarcía Arumí, JoséDuarri Piqué, AnnaDiferenciació cel·lularUlls - MalaltiesMedicina regenerativaANATOMY::Sense Organs::Eye::Pigment Epithelium of Eye::Retinal Pigment EpitheliumANATOMY::Cells::Stem Cells::Adult Stem Cells::Induced Pluripotent Stem CellsPHENOMENA AND PROCESSES::Cell Physiological Phenomena::Cell DifferentiationANATOMÍA::órganos de los sentidos::ojo::epitelio pigmentado ocular::epitelio pigmentado de la retinaANATOMÍA::células::células madre::células madre adultas::células madre pluripotentes inducidasFENÓMENOS Y PROCESOS::fenómenos fisiológicos celulares::diferenciación celularCornea; Ocular organoids; Ocular precursor cellsCórnea; Organoides oculares; Células precursoras ocularesCòrnia; Organoides oculars; Cèl·lules precursores ocularsBackground Recently, great efforts have been made to design protocols for obtaining ocular cells from human stem cells to model diseases or for regenerative purposes. Current protocols generally focus on isolating retinal cells, retinal pigment epithelium (RPE), or corneal cells and fail to recapitulate the complexity of the tissue during eye development. Here, the generation of more advanced in vitro multiocular organoids from human induced pluripotent stem cells (hiPSCs) is demonstrated. Methods A 2-step method was established to first obtain self-organized multizone ocular progenitor cells (mzOPCs) from 2D hiPSC cultures within three weeks. Then, after the cells were manually isolated and grown in suspension, 3D multiocular organoids were generated to model important cellular features of developing eyes. Results In the 2D culture, self-formed mzOPCs spanned the neuroectoderm, surface ectoderm, neural crest, and RPE, mimicking early stages of eye development. After lifting, mzOPCs developed into different 3D multiocular organoids composed of multiple cell lineages including RPE, retina, and cornea, and interactions between the different cell types and regions of the eye system were observed. Within these organoids, the retinal regions exhibited correct layering and contained all major retinal cell subtypes as well as retinal morphological cues, whereas the corneal regions closely resembled the transparent ocular-surface epithelium and contained of corneal, limbal, and conjunctival epithelial cells. The arrangement of RPE cells also formed organoids composed of polarized pigmented epithelial cells at the surface that were completely filled with collagen matrix. Conclusions This approach clearly demonstrated the advantages of the combined 2D-3D construction tissue model as it provided a more ocular native-like cellular environment than that of previous models. In this complex preparations, multiocular organoids may be used to model the crosstalk between different cell types in eye development and disease.This research project was funded by a grant from La Marató de TV3 Foundation (484/C/2012) and ERA-NET EuroNanoMed III/ISCIII (AC19/00080) (CELLUX). We thank CERCA Program/Generalitat de Catalunya for institutional support (SGR 2017–2019). A.D. was supported by PRB2-ISCIII (PT13/0001/0041) and ISCIII-FEDER RETICS (Oftared; RD16/0008). H.IM. was supported by EuroNanoMed III-ISCIII (AC19/00080).BMCInstitut Català de la Salut[Isla-Magrané H, Duarri A] Grup de Recerca en Oftalmologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. [Veiga A] Regenerative Medicine Program IDIBELL, L’Hospitalet de Llobregat, Barcelona, Spain. [García-Arumí J] Grup de Recerca en Oftalmologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei d’Oftalmologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Departament d’Oftalmologia, Universitat Autònoma de Barcelona, Bellaterra, SpainVall d'Hebron Barcelona Hospital Campus202220222021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11351/7565Scientiareponame:Scientia. Dipòsit d'Informació Digital del Departament de Salutinstname:Departament de Salut de la Generalitat de Catalunya (DS)InglésStem Cell Research & Therapy;12https://doi.org/10.1186/s13287-021-02651-9info:eu-repo/grantAgreement/ES/PE2017-2020/AC19%2F00080Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:scientiasalut.gencat.cat:11351/75652026-06-12T09:38:37Z
score 15.81155

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