Mating to intact, but not vasectomized, males elicits changes in the endometrial transcriptome: insights from the bovine model

Les dades d’expressió gènica estan disponibles públicament al repositori digital Dryad https://doi.org/10.5061/dryad.s7h44j14r i al repositori institucional DUGiDocs http://hdl.handle.net/10256/18379

Detalles Bibliográficos
Autores: Recuero, Sandra, Sánchez, José María, Mateo Otero, Yentel, Bagés-Arnal, Sandra, McDonald, Michael, Behura, Susanta K., Spencer, Thomas E., Kenny, David A., Yeste Oliveras, Marc, Lonergan, Pat, Fernández Fuertes, Beatriz
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/18305
Acceso en línea:http://hdl.handle.net/10256/18305
Access Level:acceso abierto
Palabra clave:Bestiar boví -- Espermatozoides
Cattle -- Spermatozoa
Bestiar boví -- Fecunditat
Cattle -- Fertility
Bestiar boví -- Reproducció
Cattle -- Reproduction
Endometri
Endometrium
RNA
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oai_identifier_str oai:recercat.cat:10256/18305
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Mating to intact, but not vasectomized, males elicits changes in the endometrial transcriptome: insights from the bovine model
title Mating to intact, but not vasectomized, males elicits changes in the endometrial transcriptome: insights from the bovine model
spellingShingle Mating to intact, but not vasectomized, males elicits changes in the endometrial transcriptome: insights from the bovine model
Recuero, Sandra
Bestiar boví -- Espermatozoides
Cattle -- Spermatozoa
Bestiar boví -- Fecunditat
Cattle -- Fertility
Bestiar boví -- Reproducció
Cattle -- Reproduction
Endometri
Endometrium
RNA
title_short Mating to intact, but not vasectomized, males elicits changes in the endometrial transcriptome: insights from the bovine model
title_full Mating to intact, but not vasectomized, males elicits changes in the endometrial transcriptome: insights from the bovine model
title_fullStr Mating to intact, but not vasectomized, males elicits changes in the endometrial transcriptome: insights from the bovine model
title_full_unstemmed Mating to intact, but not vasectomized, males elicits changes in the endometrial transcriptome: insights from the bovine model
title_sort Mating to intact, but not vasectomized, males elicits changes in the endometrial transcriptome: insights from the bovine model
dc.creator.none.fl_str_mv Recuero, Sandra
Sánchez, José María
Mateo Otero, Yentel
Bagés-Arnal, Sandra
McDonald, Michael
Behura, Susanta K.
Spencer, Thomas E.
Kenny, David A.
Yeste Oliveras, Marc
Lonergan, Pat
Fernández Fuertes, Beatriz
author Recuero, Sandra
author_facet Recuero, Sandra
Sánchez, José María
Mateo Otero, Yentel
Bagés-Arnal, Sandra
McDonald, Michael
Behura, Susanta K.
Spencer, Thomas E.
Kenny, David A.
Yeste Oliveras, Marc
Lonergan, Pat
Fernández Fuertes, Beatriz
author_role author
author2 Sánchez, José María
Mateo Otero, Yentel
Bagés-Arnal, Sandra
McDonald, Michael
Behura, Susanta K.
Spencer, Thomas E.
Kenny, David A.
Yeste Oliveras, Marc
Lonergan, Pat
Fernández Fuertes, Beatriz
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bestiar boví -- Espermatozoides
Cattle -- Spermatozoa
Bestiar boví -- Fecunditat
Cattle -- Fertility
Bestiar boví -- Reproducció
Cattle -- Reproduction
Endometri
Endometrium
RNA
topic Bestiar boví -- Espermatozoides
Cattle -- Spermatozoa
Bestiar boví -- Fecunditat
Cattle -- Fertility
Bestiar boví -- Reproducció
Cattle -- Reproduction
Endometri
Endometrium
RNA
description Les dades d’expressió gènica estan disponibles públicament al repositori digital Dryad https://doi.org/10.5061/dryad.s7h44j14r i al repositori institucional DUGiDocs http://hdl.handle.net/10256/18379
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/18305
http://hdl.handle.net/10256/18305
url http://hdl.handle.net/10256/18305
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fcell.2020.00547
info:eu-repo/semantics/altIdentifier/eissn/2296-634X
info:eu-repo/grantAgreement/EC/H2020/792212
info:eu-repo/semantics/datase/handle/10256/18379
dc.rights.none.fl_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Frontiers In Cell and Developmental Biology, 2020, vol. 8, art.núm. 547
Articles publicats (D-B)
Recuero, Sandra Sánchez, José María Mateo Otero, Yentel Bagés-Arnal, Sandra McDonald, Michael Behura, Susanta K. Spencer, Thomas E. Kenny, David A. Yeste Oliveras, Marc Lonergan, Pat Fernández Fuertes, Beatriz 2020 Mating to intact, but not vasectomized, males elicits changes in the endometrial transcriptome: insights from the bovine model Frontiers In Cell And Developmental Biology 8 Art.núm. 547
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Mating to intact, but not vasectomized, males elicits changes in the endometrial transcriptome: insights from the bovine modelRecuero, SandraSánchez, José MaríaMateo Otero, YentelBagés-Arnal, SandraMcDonald, MichaelBehura, Susanta K.Spencer, Thomas E.Kenny, David A.Yeste Oliveras, MarcLonergan, PatFernández Fuertes, BeatrizBestiar boví -- EspermatozoidesCattle -- SpermatozoaBestiar boví -- FecunditatCattle -- FertilityBestiar boví -- ReproduccióCattle -- ReproductionEndometriEndometriumRNALes dades d’expressió gènica estan disponibles públicament al repositori digital Dryad https://doi.org/10.5061/dryad.s7h44j14r i al repositori institucional DUGiDocs http://hdl.handle.net/10256/18379An appropriate female reproductive environment is essential for pregnancy success. In several species, including mice, pigs and horses, seminal plasma (SP) components have been shown to modulate this environment, leading to increased embryo viability and implantation. Due to the characteristics of mating in the aforementioned species, SP comes into direct contact with the uterus. However, it is questionable whether any SP reaches the uterus in species that ejaculate inside the vagina, such as humans and cattle. Hence, we hypothesized that sperm, perhaps acting as a vehicle for SP factors, play a more important role in the modulation of the maternal uterine environment in these species. In addition, changes elicited by SP and/or sperm may originate in the vagina and propagate to more distal regions of the female reproductive tract. To test these hypotheses, a bovine model in which heifers were mated to intact or vasectomized bulls or were left unmated was used. RNA-sequencing of endometrial samples collected 24 h after mating with a vasectomized bull did not reveal any differentially expressed genes (DEGs) in comparison with control samples. However, the endometrium of heifers mated with intact bulls exhibited 24 DEGs when compared to heifers mated with vasectomized bulls, and 22 DEGs when compared to unmated control heifers. The expression of a set of cytokines (IL6, IL1A, IL8, and TNFA) and candidate genes identified in the endometrial RNA-sequencing (PLA2G10, CX3CL1, C4BPA, PRSS2, BLA-DQB, and CEBPD) were assessed by RT-qPCR in the vagina and oviductal ampulla. No differences in expression of these genes were observed between treatments in any region. However, mating to both intact and vasectomized bulls induced an increase in IL1A and TNFA expression in the vagina compared to the oviduct. These data indicate that sperm, but not secretions from the accessory glands alone, induce modest changes in endometrial gene expression after natural mating in cattle. However, it is not clear whether this effect is triggered by inherent sperm proteins or SP proteins bound to sperm surface at the time of ejaculationThis work was funded by the EU Horizon 2020 Marie Skłodowska-Curie (No. 792212) and Science Foundation Ireland (Grant No. 16/IA/4474)2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/18305http://hdl.handle.net/10256/18305Frontiers In Cell and Developmental Biology, 2020, vol. 8, art.núm. 547Articles publicats (D-B)Recuero, Sandra Sánchez, José María Mateo Otero, Yentel Bagés-Arnal, Sandra McDonald, Michael Behura, Susanta K. Spencer, Thomas E. Kenny, David A. Yeste Oliveras, Marc Lonergan, Pat Fernández Fuertes, Beatriz 2020 Mating to intact, but not vasectomized, males elicits changes in the endometrial transcriptome: insights from the bovine model Frontiers In Cell And Developmental Biology 8 Art.núm. 547reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fcell.2020.00547info:eu-repo/semantics/altIdentifier/eissn/2296-634Xinfo:eu-repo/grantAgreement/EC/H2020/792212info:eu-repo/semantics/datase/handle/10256/18379Reconeixement 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:recercat.cat:10256/183052026-05-29T05:05:01Z
score 15.81155

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