Leptin administration favors muscle mass accretion by decreasing FoxO3a and increasing PGC-1alpha in ob/ob mice
Absence of leptin has been associated with reduced skeletal muscle mass in leptin-deficient ob/ob mice. The aim of our study was to examine the effect of leptin on the catabolic and anabolic pathways regulating muscle mass. Gastrocnemius, extensor digitorum longus and soleus muscle mass as well as f...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2009 |
| País: | España |
| Institución: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/36330 |
| Acceso en línea: | https://hdl.handle.net/10171/36330 |
| Access Level: | acceso abierto |
| Palabra clave: | Leptin Myostatin FoxO3 protein, mouse Muscles/pathology Gene Expression Profiling Troponin T/biosynthesis Mice |
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Leptin administration favors muscle mass accretion by decreasing FoxO3a and increasing PGC-1alpha in ob/ob miceSainz-Amillo, N. (Neira)|||/items/489b1d3f-6e84-4dfc-8d98-11d6340fd177Rodriguez, A. (Amaia)|||/items/50976a5f-9842-47f1-8a9c-508e59996427Catalán-Goñi, V. (Victoria)|||/items/9a5a055c-323a-4b41-800f-65abf7f22c79Becerril-Mañas, S. (Sara)|||/items/5edb25ec-0edd-44eb-8ef1-c81d0ebd7300Ramirez-Sola, B. (Beatriz)|||/items/95970833-a061-4e49-b5d8-e6ecc6ec59c4Gomez-Ambrosi, J. (Javier)|||/items/21d09997-940a-45f7-9b4b-0a9f64b2ed8eFrühbeck, G. (Gema)|||/items/7f0b1f72-bc91-4ab0-a3fd-21e9a3fb663bLeptinMyostatinFoxO3 protein, mouseMuscles/pathologyGene Expression ProfilingTroponin T/biosynthesisMiceAbsence of leptin has been associated with reduced skeletal muscle mass in leptin-deficient ob/ob mice. The aim of our study was to examine the effect of leptin on the catabolic and anabolic pathways regulating muscle mass. Gastrocnemius, extensor digitorum longus and soleus muscle mass as well as fiber size were significantly lower in ob/ob mice compared to wild type littermates, being significantly increased by leptin administration (P<0.001). This effect was associated with an inactivation of the muscle atrophy-related transcription factor forkhead box class O3 (FoxO3a) (P<0.05), and with a decrease in the protein expression levels of the E3 ubiquitin-ligases muscle atrophy F-box (MAFbx) (P<0.05) and muscle RING finger 1 (MuRF1) (P<0.05). Moreover, leptin increased (P<0.01) protein expression levels of peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha), a regulator of muscle fiber type, and decreased (P<0.05) myostatin protein, a negative regulator of muscle growth. Leptin administration also activated (P<0.01) the regulators of cell cycle progression proliferating cell nuclear antigen (PCNA) and cyclin D1, and increased (P<0.01) myofibrillar protein troponin T. The present study provides evidence that leptin treatment may increase muscle mass of ob/ob mice by inhibiting myofibrillar protein degradation as well as enhancing muscle cell proliferation.Public Library of ScienceDadun. Depósito Académico Digital Universidad de Navarra20142014-08-1920092009-01-0120092009-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/36330reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/363302026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Leptin administration favors muscle mass accretion by decreasing FoxO3a and increasing PGC-1alpha in ob/ob mice |
| title |
Leptin administration favors muscle mass accretion by decreasing FoxO3a and increasing PGC-1alpha in ob/ob mice |
| spellingShingle |
Leptin administration favors muscle mass accretion by decreasing FoxO3a and increasing PGC-1alpha in ob/ob mice Sainz-Amillo, N. (Neira)|||/items/489b1d3f-6e84-4dfc-8d98-11d6340fd177 Leptin Myostatin FoxO3 protein, mouse Muscles/pathology Gene Expression Profiling Troponin T/biosynthesis Mice |
| title_short |
Leptin administration favors muscle mass accretion by decreasing FoxO3a and increasing PGC-1alpha in ob/ob mice |
| title_full |
Leptin administration favors muscle mass accretion by decreasing FoxO3a and increasing PGC-1alpha in ob/ob mice |
| title_fullStr |
Leptin administration favors muscle mass accretion by decreasing FoxO3a and increasing PGC-1alpha in ob/ob mice |
| title_full_unstemmed |
Leptin administration favors muscle mass accretion by decreasing FoxO3a and increasing PGC-1alpha in ob/ob mice |
| title_sort |
Leptin administration favors muscle mass accretion by decreasing FoxO3a and increasing PGC-1alpha in ob/ob mice |
| dc.creator.none.fl_str_mv |
Sainz-Amillo, N. (Neira)|||/items/489b1d3f-6e84-4dfc-8d98-11d6340fd177 Rodriguez, A. (Amaia)|||/items/50976a5f-9842-47f1-8a9c-508e59996427 Catalán-Goñi, V. (Victoria)|||/items/9a5a055c-323a-4b41-800f-65abf7f22c79 Becerril-Mañas, S. (Sara)|||/items/5edb25ec-0edd-44eb-8ef1-c81d0ebd7300 Ramirez-Sola, B. (Beatriz)|||/items/95970833-a061-4e49-b5d8-e6ecc6ec59c4 Gomez-Ambrosi, J. (Javier)|||/items/21d09997-940a-45f7-9b4b-0a9f64b2ed8e Frühbeck, G. (Gema)|||/items/7f0b1f72-bc91-4ab0-a3fd-21e9a3fb663b |
| author |
Sainz-Amillo, N. (Neira)|||/items/489b1d3f-6e84-4dfc-8d98-11d6340fd177 |
| author_facet |
Sainz-Amillo, N. (Neira)|||/items/489b1d3f-6e84-4dfc-8d98-11d6340fd177 Rodriguez, A. (Amaia)|||/items/50976a5f-9842-47f1-8a9c-508e59996427 Catalán-Goñi, V. (Victoria)|||/items/9a5a055c-323a-4b41-800f-65abf7f22c79 Becerril-Mañas, S. (Sara)|||/items/5edb25ec-0edd-44eb-8ef1-c81d0ebd7300 Ramirez-Sola, B. (Beatriz)|||/items/95970833-a061-4e49-b5d8-e6ecc6ec59c4 Gomez-Ambrosi, J. (Javier)|||/items/21d09997-940a-45f7-9b4b-0a9f64b2ed8e Frühbeck, G. (Gema)|||/items/7f0b1f72-bc91-4ab0-a3fd-21e9a3fb663b |
| author_role |
author |
| author2 |
Rodriguez, A. (Amaia)|||/items/50976a5f-9842-47f1-8a9c-508e59996427 Catalán-Goñi, V. (Victoria)|||/items/9a5a055c-323a-4b41-800f-65abf7f22c79 Becerril-Mañas, S. (Sara)|||/items/5edb25ec-0edd-44eb-8ef1-c81d0ebd7300 Ramirez-Sola, B. (Beatriz)|||/items/95970833-a061-4e49-b5d8-e6ecc6ec59c4 Gomez-Ambrosi, J. (Javier)|||/items/21d09997-940a-45f7-9b4b-0a9f64b2ed8e Frühbeck, G. (Gema)|||/items/7f0b1f72-bc91-4ab0-a3fd-21e9a3fb663b |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Leptin Myostatin FoxO3 protein, mouse Muscles/pathology Gene Expression Profiling Troponin T/biosynthesis Mice |
| topic |
Leptin Myostatin FoxO3 protein, mouse Muscles/pathology Gene Expression Profiling Troponin T/biosynthesis Mice |
| description |
Absence of leptin has been associated with reduced skeletal muscle mass in leptin-deficient ob/ob mice. The aim of our study was to examine the effect of leptin on the catabolic and anabolic pathways regulating muscle mass. Gastrocnemius, extensor digitorum longus and soleus muscle mass as well as fiber size were significantly lower in ob/ob mice compared to wild type littermates, being significantly increased by leptin administration (P<0.001). This effect was associated with an inactivation of the muscle atrophy-related transcription factor forkhead box class O3 (FoxO3a) (P<0.05), and with a decrease in the protein expression levels of the E3 ubiquitin-ligases muscle atrophy F-box (MAFbx) (P<0.05) and muscle RING finger 1 (MuRF1) (P<0.05). Moreover, leptin increased (P<0.01) protein expression levels of peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha), a regulator of muscle fiber type, and decreased (P<0.05) myostatin protein, a negative regulator of muscle growth. Leptin administration also activated (P<0.01) the regulators of cell cycle progression proliferating cell nuclear antigen (PCNA) and cyclin D1, and increased (P<0.01) myofibrillar protein troponin T. The present study provides evidence that leptin treatment may increase muscle mass of ob/ob mice by inhibiting myofibrillar protein degradation as well as enhancing muscle cell proliferation. |
| publishDate |
2009 |
| dc.date.none.fl_str_mv |
2009 2009-01-01 2009 2009-01-01 2014 2014-08-19 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/36330 |
| url |
https://hdl.handle.net/10171/36330 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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Public Library of Science |
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Public Library of Science |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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1869405563507441664 |
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15.300719 |