The Role of Viral and Host MicroRNAs in the Aujeszky’s Disease Virus during the Infection Process

Porcine production is a primary market in the world economy. Controlling swine diseases in the farm is essential in order to achieve the sector necessities. Aujeszky’s disease is a viral condition affecting pigs and is endemic in many countries of the world, causing important economic losses in the...

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Autores: Timoneda, Oriol, Núñez-Hernández, Fernando, Balcells, Ingrid, Muñoz, Marta, Castelló, Anna, Vera, Gonzalo, Pérez, Lester J., Egea, Raquel, Mir, Gisela, Córdoba, Sarai, Rosell, Rosa, Segalés, Joaquim, Tomás, Anna, Sánchez, Armand, Núñez, J Ignacio
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/3216
Acceso en línea:http://hdl.handle.net/20.500.12327/3216
https://doi.org/10.1371/journal.pone.0086965
Access Level:acceso abierto
Palabra clave:619
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spelling The Role of Viral and Host MicroRNAs in the Aujeszky’s Disease Virus during the Infection ProcessTimoneda, OriolNúñez-Hernández, FernandoBalcells, IngridMuñoz, MartaCastelló, AnnaVera, GonzaloPérez, Lester J.Egea, RaquelMir, GiselaCórdoba, SaraiRosell, RosaSegalés, JoaquimTomás, AnnaSánchez, ArmandNúñez, J Ignacio619Porcine production is a primary market in the world economy. Controlling swine diseases in the farm is essential in order to achieve the sector necessities. Aujeszky’s disease is a viral condition affecting pigs and is endemic in many countries of the world, causing important economic losses in the swine industry. microRNAs (miRNAs) are non-coding RNAs which modulates gene expression in animals, plants and viruses. With the aim of understanding miRNA roles during the Aujeszky’s disease virus [ADV] (also known as suid herpesvirus type 1 [SuHV-1]) infection, the expression profiles of host and viral miRNAs were determined through deep sequencing in SuHV-1 infected porcine cell line (PK-15) and in an animal experimental SuHV-1 infection with virulent (NIA-3) and attenuated (Begonia) strains. In the in vivo approach miR-206, miR-133a, miR-133b and miR-378 presented differential expression between virus strains infection. In the in vitro approach, most miRNAs were down-regulated in infected groups. miR-92a and miR-92b-3p were up-regulated in Begonia infected samples. Functional analysis of all this over expressed miRNAs during the infection revealed their association in pathways related to viral infection processes and immune response. Furthermore, 8 viral miRNAs were detected by stem loop RT-qPCR in both in vitro and in vivo approaches, presenting a gene regulatory network affecting 59 viral genes. Most described viral miRNAs were related to Large Latency Transcript (LLT) and to viral transcription activators EP0 and IE180, and also to regulatory genes regarding their important roles in the host – pathogen interaction during viral infection.info:eu-repo/semantics/publishedVersionPublic Library of ScienceProducció AnimalSanitat Animal2014info:eu-repo/semantics/article11http://hdl.handle.net/20.500.12327/3216https://doi.org/10.1371/journal.pone.0086965reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésPLoS ONEMEC/ /AGL2007-66371-C02/ES/IDENTIFICACION Y CARACTERIZACION DE MICRORNAS VIRICOS QUE AFECTAN AL PORCINO/MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-22358-C02/ES/MICRORNAS EN INFECCIONES VIRICAS DEL CERDO: ANALISIS FUNCIONAL E IMPLICACIONES EN PATOGENIA VIRAL/Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/32162026-05-29T05:05:01Z
dc.title.none.fl_str_mv The Role of Viral and Host MicroRNAs in the Aujeszky’s Disease Virus during the Infection Process
title The Role of Viral and Host MicroRNAs in the Aujeszky’s Disease Virus during the Infection Process
spellingShingle The Role of Viral and Host MicroRNAs in the Aujeszky’s Disease Virus during the Infection Process
Timoneda, Oriol
619
title_short The Role of Viral and Host MicroRNAs in the Aujeszky’s Disease Virus during the Infection Process
title_full The Role of Viral and Host MicroRNAs in the Aujeszky’s Disease Virus during the Infection Process
title_fullStr The Role of Viral and Host MicroRNAs in the Aujeszky’s Disease Virus during the Infection Process
title_full_unstemmed The Role of Viral and Host MicroRNAs in the Aujeszky’s Disease Virus during the Infection Process
title_sort The Role of Viral and Host MicroRNAs in the Aujeszky’s Disease Virus during the Infection Process
dc.creator.none.fl_str_mv Timoneda, Oriol
Núñez-Hernández, Fernando
Balcells, Ingrid
Muñoz, Marta
Castelló, Anna
Vera, Gonzalo
Pérez, Lester J.
Egea, Raquel
Mir, Gisela
Córdoba, Sarai
Rosell, Rosa
Segalés, Joaquim
Tomás, Anna
Sánchez, Armand
Núñez, J Ignacio
author Timoneda, Oriol
author_facet Timoneda, Oriol
Núñez-Hernández, Fernando
Balcells, Ingrid
Muñoz, Marta
Castelló, Anna
Vera, Gonzalo
Pérez, Lester J.
Egea, Raquel
Mir, Gisela
Córdoba, Sarai
Rosell, Rosa
Segalés, Joaquim
Tomás, Anna
Sánchez, Armand
Núñez, J Ignacio
author_role author
author2 Núñez-Hernández, Fernando
Balcells, Ingrid
Muñoz, Marta
Castelló, Anna
Vera, Gonzalo
Pérez, Lester J.
Egea, Raquel
Mir, Gisela
Córdoba, Sarai
Rosell, Rosa
Segalés, Joaquim
Tomás, Anna
Sánchez, Armand
Núñez, J Ignacio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Sanitat Animal
dc.subject.none.fl_str_mv 619
topic 619
description Porcine production is a primary market in the world economy. Controlling swine diseases in the farm is essential in order to achieve the sector necessities. Aujeszky’s disease is a viral condition affecting pigs and is endemic in many countries of the world, causing important economic losses in the swine industry. microRNAs (miRNAs) are non-coding RNAs which modulates gene expression in animals, plants and viruses. With the aim of understanding miRNA roles during the Aujeszky’s disease virus [ADV] (also known as suid herpesvirus type 1 [SuHV-1]) infection, the expression profiles of host and viral miRNAs were determined through deep sequencing in SuHV-1 infected porcine cell line (PK-15) and in an animal experimental SuHV-1 infection with virulent (NIA-3) and attenuated (Begonia) strains. In the in vivo approach miR-206, miR-133a, miR-133b and miR-378 presented differential expression between virus strains infection. In the in vitro approach, most miRNAs were down-regulated in infected groups. miR-92a and miR-92b-3p were up-regulated in Begonia infected samples. Functional analysis of all this over expressed miRNAs during the infection revealed their association in pathways related to viral infection processes and immune response. Furthermore, 8 viral miRNAs were detected by stem loop RT-qPCR in both in vitro and in vivo approaches, presenting a gene regulatory network affecting 59 viral genes. Most described viral miRNAs were related to Large Latency Transcript (LLT) and to viral transcription activators EP0 and IE180, and also to regulatory genes regarding their important roles in the host – pathogen interaction during viral infection.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/3216
https://doi.org/10.1371/journal.pone.0086965
url http://hdl.handle.net/20.500.12327/3216
https://doi.org/10.1371/journal.pone.0086965
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PLoS ONE
MEC/ /AGL2007-66371-C02/ES/IDENTIFICACION Y CARACTERIZACION DE MICRORNAS VIRICOS QUE AFECTAN AL PORCINO/
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-22358-C02/ES/MICRORNAS EN INFECCIONES VIRICAS DEL CERDO: ANALISIS FUNCIONAL E IMPLICACIONES EN PATOGENIA VIRAL/
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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