In vitro culture of ovine embryos up to early gastrulating stages

11 Pág.

Detalhes bibliográficos
Autores: Ramos Ibeas, Priscila, González-Brusi, Leopoldo, Used, María Torres, Cocero Oviedo, María Jesús, Marigorta, Pilar, Alberio, Ramiro, Bermejo Álvarez, Pablo
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/273101
Acesso em linha:http://hdl.handle.net/10261/273101
https://api.elsevier.com/content/abstract/scopus_id/85126895798
Access Level:acceso abierto
Palavra-chave:In vitro
Embryo
Gastrulation
Ovine
Post-hatching culture
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spelling In vitro culture of ovine embryos up to early gastrulating stagesRamos Ibeas, PriscilaGonzález-Brusi, LeopoldoUsed, María TorresCocero Oviedo, María JesúsMarigorta, PilarAlberio, RamiroBermejo Álvarez, PabloIn vitroEmbryoGastrulationOvinePost-hatching culture11 Pág.Developmental failures occurring shortly after blastocyst hatching from the zona pellucida constitute a major cause of pregnancy losses in both humans and farm ungulates. The developmental events occurring following hatching in ungulates include the proliferation and maturation of extra-embryonic membranes - trophoblast and hypoblast - and the formation of a flat embryonic disc, similar to that found in humans, which initiates gastrulation prior to implantation. Unfortunately, our understanding of these key processes for embryo survival is limited because current culture systems cannot sustain ungulate embryo development beyond hatching. Here, we report a culture system that recapitulates most developmental landmarks of gastrulating ovine embryos: trophoblast maturation, hypoblast migration, embryonic disc formation, disappearance of the Rauber's layer, epiblast polarization and mesoderm differentiation. Our system represents a highly valuable platform for exploring the cell differentiation, proliferation and migration processes governing gastrulation in a flat embryonic disc and for understanding pregnancy failures during the second week of gestation. This article has an associated 'The people behind the papers' interview.This work was funded by the European Research Council (StG-757886-ELONGAN), the Ministerio de Economía y Competitividad (MINECO; AGL2017-58739-R) and the Spanish Ministerio de Ciencia e Innovación (ECQ2018-005184-P) to P.B.Á. P.R.-I. was funded by a Ramón y Cajal Contract from MINECO (RYC2018-025666-I). Open Access funding provided by Consejo Superior de Investigaciones Científicas (CSIC).Peer reviewedCompany of BiologistsEuropean CommissionMinisterio de Economía y Competitividad (España)Ministerio de Ciencia e Innovación (España)Ramos Ibeas, Priscila [0000-0003-4578-0189]González-Brusi, Leopoldo [0000-0002-6371-6423]Cocero Oviedo, María Jesús [0000-0002-6254-3057]Marigorta, Pilar [0000-0002-6287-5780]Alberio, Ramiro [0000-0001-6560-3919]Bermejo Álvarez, Pablo [0000-0001-9907-2626]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/273101https://api.elsevier.com/content/abstract/scopus_id/85126895798reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC//StG-757886-ELONGANinfo:eu-repo/grantAgreement/MINECO//AGL2017-58739-Rinfo:eu-repo/grantAgreement/MICINN//ECQ2018-005184-Pinfo:eu-repo/grantAgreement/MINECO//RYC2018-025666-IDevelopment (Cambridge, England)https://doi.org/10.1242/dev.199743Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2731012026-05-22T06:33:51Z
dc.title.none.fl_str_mv In vitro culture of ovine embryos up to early gastrulating stages
title In vitro culture of ovine embryos up to early gastrulating stages
spellingShingle In vitro culture of ovine embryos up to early gastrulating stages
Ramos Ibeas, Priscila
In vitro
Embryo
Gastrulation
Ovine
Post-hatching culture
title_short In vitro culture of ovine embryos up to early gastrulating stages
title_full In vitro culture of ovine embryos up to early gastrulating stages
title_fullStr In vitro culture of ovine embryos up to early gastrulating stages
title_full_unstemmed In vitro culture of ovine embryos up to early gastrulating stages
title_sort In vitro culture of ovine embryos up to early gastrulating stages
dc.creator.none.fl_str_mv Ramos Ibeas, Priscila
González-Brusi, Leopoldo
Used, María Torres
Cocero Oviedo, María Jesús
Marigorta, Pilar
Alberio, Ramiro
Bermejo Álvarez, Pablo
author Ramos Ibeas, Priscila
author_facet Ramos Ibeas, Priscila
González-Brusi, Leopoldo
Used, María Torres
Cocero Oviedo, María Jesús
Marigorta, Pilar
Alberio, Ramiro
Bermejo Álvarez, Pablo
author_role author
author2 González-Brusi, Leopoldo
Used, María Torres
Cocero Oviedo, María Jesús
Marigorta, Pilar
Alberio, Ramiro
Bermejo Álvarez, Pablo
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia e Innovación (España)
Ramos Ibeas, Priscila [0000-0003-4578-0189]
González-Brusi, Leopoldo [0000-0002-6371-6423]
Cocero Oviedo, María Jesús [0000-0002-6254-3057]
Marigorta, Pilar [0000-0002-6287-5780]
Alberio, Ramiro [0000-0001-6560-3919]
Bermejo Álvarez, Pablo [0000-0001-9907-2626]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv In vitro
Embryo
Gastrulation
Ovine
Post-hatching culture
topic In vitro
Embryo
Gastrulation
Ovine
Post-hatching culture
description 11 Pág.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/273101
https://api.elsevier.com/content/abstract/scopus_id/85126895798
url http://hdl.handle.net/10261/273101
https://api.elsevier.com/content/abstract/scopus_id/85126895798
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC//StG-757886-ELONGAN
info:eu-repo/grantAgreement/MINECO//AGL2017-58739-R
info:eu-repo/grantAgreement/MICINN//ECQ2018-005184-P
info:eu-repo/grantAgreement/MINECO//RYC2018-025666-I
Development (Cambridge, England)
https://doi.org/10.1242/dev.199743
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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