A catalogue of 863 Rett-syndrome-causing MECP2 mutations and lessons learned from data integration

Rett syndrome (RTT) is a rare neurological disorder mostly caused by a genetic variation in MECP2. Making new MECP2 variants and the related phenotypes available provides data for better understanding of disease mechanisms and faster identification of variants for diagnosis. This is, however, curren...

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Autores: Ehrhart, Friederike, Jacobsen, Annika, Rigau, Maria|||0000-0002-6501-7212, Bosio, Mattia, Kaliyaperumal, Rajaram, Laros, Jeroen F. J., Willighagen, Egon L., Valencia, Alfonso|||0000-0002-8937-6789, Roos, Marco, Capella Gutiérrez, Salvador|||0000-0002-0309-604X, Curfs, Leopold M. G., Evelo, Chris T.
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/338157
Acceso en línea:https://hdl.handle.net/2117/338157
https://dx.doi.org/10.1038/s41597-020-00794-7
Access Level:acceso abierto
Palabra clave:Rett syndrome
Methyl-CpG-binding protein 2
Data sets
Nervous system--Diseases
Data integration
Disease genetics
Genetics research
Neurological disorders
Genètica
Àrees temàtiques de la UPC::Informàtica::Aplicacions de la informàtica::Bioinformàtica
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spelling A catalogue of 863 Rett-syndrome-causing MECP2 mutations and lessons learned from data integrationEhrhart, FriederikeJacobsen, AnnikaRigau, Maria|||0000-0002-6501-7212Bosio, MattiaKaliyaperumal, RajaramLaros, Jeroen F. J.Willighagen, Egon L.Valencia, Alfonso|||0000-0002-8937-6789Roos, MarcoCapella Gutiérrez, Salvador|||0000-0002-0309-604XCurfs, Leopold M. G.Evelo, Chris T.Rett syndromeMethyl-CpG-binding protein 2Data setsNervous system--DiseasesData integrationDisease geneticsGenetics researchNeurological disordersGenèticaÀrees temàtiques de la UPC::Informàtica::Aplicacions de la informàtica::BioinformàticaRett syndrome (RTT) is a rare neurological disorder mostly caused by a genetic variation in MECP2. Making new MECP2 variants and the related phenotypes available provides data for better understanding of disease mechanisms and faster identification of variants for diagnosis. This is, however, currently hampered by the lack of interoperability between genotype-phenotype databases. Here, we demonstrate on the example of MECP2 in RTT that by making the genotype-phenotype data more Findable, Accessible, Interoperable, and Reusable (FAIR), we can facilitate prioritization and analysis of variants. In total, 10,968 MECP2 variants were successfully integrated. Among these variants 863 unique confirmed RTT causing and 209 unique confirmed benign variants were found. This dataset was used for comparison of pathogenicity predicting tools, protein consequences, and identification of ambiguous variants. Prediction tools generally recognised the RTT causing and benign variants, however, there was a broad range of overlap Nineteen variants were identified that were annotated as both disease-causing and benign, suggesting that there are additional factors in these cases contributing to disease development.The authors would like to thank the Mutalyzer team for support and feedback, Henk van Kranen for support in liftover of ancient genetic variant descriptions, and Eric Smeets for collection of the Maastricht Rett dataset.This work was funded by ELIXIR (funded by the European Commission within the Research Infrastructures programme of Horizon 2020), the research infrastructure for life-science data (MolData2). FE and LC were also funded by The Dutch Rett Syndrome Foundation (Stichting Terre). CE, AJ, RK, AV, SCG, MB, MRi and MR also received funding from EXCELERATE (H2020, Grant No. 676559). AJ, RK, MR, MB, and SCG also received funding from RD-Connect, European Union Seventh Framework Programme (FP7/2007–2013, Grant No. 305444). FE, CE, AJ, RK, MR, MB, and SCG received funding from the European Union’s Horizon 2020 Research and Innovation Program under grant agreement EJP RD N°825575. RK was also funded by NWO in project VWData (grant no. 400.17.605) and BBMRI-NL (NWO, National Roadmap for Large-Scale Research Facilities, grant no. 184.033.111). AV and SCG also received funding from INB Grant (Grant No. PT17/0009/0001 - ISCIII-SGEFI / ERDF).Peer ReviewedSpringer Nature20212021-01-0120212021-02-09journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/338157https://dx.doi.org/10.1038/s41597-020-00794-7reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)InglésengEuropean Commission http://doi.org/10.13039/100010661 Horizon 2020 Framework Programme 676559 ELIXIR-EXCELERATE: Fast-track ELIXIR implementation and drive early user exploitation across the life-sciences.open accesshttp://purl.org/coar/access_right/c_abf2Attribution 3.0 Spainhttp://creativecommons.org/licenses/by/3.0/es/Attribution 3.0 Spainhttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3381572026-05-27T15:37:01Z
dc.title.none.fl_str_mv A catalogue of 863 Rett-syndrome-causing MECP2 mutations and lessons learned from data integration
title A catalogue of 863 Rett-syndrome-causing MECP2 mutations and lessons learned from data integration
spellingShingle A catalogue of 863 Rett-syndrome-causing MECP2 mutations and lessons learned from data integration
Ehrhart, Friederike
Rett syndrome
Methyl-CpG-binding protein 2
Data sets
Nervous system--Diseases
Data integration
Disease genetics
Genetics research
Neurological disorders
Genètica
Àrees temàtiques de la UPC::Informàtica::Aplicacions de la informàtica::Bioinformàtica
title_short A catalogue of 863 Rett-syndrome-causing MECP2 mutations and lessons learned from data integration
title_full A catalogue of 863 Rett-syndrome-causing MECP2 mutations and lessons learned from data integration
title_fullStr A catalogue of 863 Rett-syndrome-causing MECP2 mutations and lessons learned from data integration
title_full_unstemmed A catalogue of 863 Rett-syndrome-causing MECP2 mutations and lessons learned from data integration
title_sort A catalogue of 863 Rett-syndrome-causing MECP2 mutations and lessons learned from data integration
dc.creator.none.fl_str_mv Ehrhart, Friederike
Jacobsen, Annika
Rigau, Maria|||0000-0002-6501-7212
Bosio, Mattia
Kaliyaperumal, Rajaram
Laros, Jeroen F. J.
Willighagen, Egon L.
Valencia, Alfonso|||0000-0002-8937-6789
Roos, Marco
Capella Gutiérrez, Salvador|||0000-0002-0309-604X
Curfs, Leopold M. G.
Evelo, Chris T.
author Ehrhart, Friederike
author_facet Ehrhart, Friederike
Jacobsen, Annika
Rigau, Maria|||0000-0002-6501-7212
Bosio, Mattia
Kaliyaperumal, Rajaram
Laros, Jeroen F. J.
Willighagen, Egon L.
Valencia, Alfonso|||0000-0002-8937-6789
Roos, Marco
Capella Gutiérrez, Salvador|||0000-0002-0309-604X
Curfs, Leopold M. G.
Evelo, Chris T.
author_role author
author2 Jacobsen, Annika
Rigau, Maria|||0000-0002-6501-7212
Bosio, Mattia
Kaliyaperumal, Rajaram
Laros, Jeroen F. J.
Willighagen, Egon L.
Valencia, Alfonso|||0000-0002-8937-6789
Roos, Marco
Capella Gutiérrez, Salvador|||0000-0002-0309-604X
Curfs, Leopold M. G.
Evelo, Chris T.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Rett syndrome
Methyl-CpG-binding protein 2
Data sets
Nervous system--Diseases
Data integration
Disease genetics
Genetics research
Neurological disorders
Genètica
Àrees temàtiques de la UPC::Informàtica::Aplicacions de la informàtica::Bioinformàtica
topic Rett syndrome
Methyl-CpG-binding protein 2
Data sets
Nervous system--Diseases
Data integration
Disease genetics
Genetics research
Neurological disorders
Genètica
Àrees temàtiques de la UPC::Informàtica::Aplicacions de la informàtica::Bioinformàtica
description Rett syndrome (RTT) is a rare neurological disorder mostly caused by a genetic variation in MECP2. Making new MECP2 variants and the related phenotypes available provides data for better understanding of disease mechanisms and faster identification of variants for diagnosis. This is, however, currently hampered by the lack of interoperability between genotype-phenotype databases. Here, we demonstrate on the example of MECP2 in RTT that by making the genotype-phenotype data more Findable, Accessible, Interoperable, and Reusable (FAIR), we can facilitate prioritization and analysis of variants. In total, 10,968 MECP2 variants were successfully integrated. Among these variants 863 unique confirmed RTT causing and 209 unique confirmed benign variants were found. This dataset was used for comparison of pathogenicity predicting tools, protein consequences, and identification of ambiguous variants. Prediction tools generally recognised the RTT causing and benign variants, however, there was a broad range of overlap Nineteen variants were identified that were annotated as both disease-causing and benign, suggesting that there are additional factors in these cases contributing to disease development.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01
2021
2021-02-09
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/338157
https://dx.doi.org/10.1038/s41597-020-00794-7
url https://hdl.handle.net/2117/338157
https://dx.doi.org/10.1038/s41597-020-00794-7
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission http://doi.org/10.13039/100010661 Horizon 2020 Framework Programme 676559 ELIXIR-EXCELERATE: Fast-track ELIXIR implementation and drive early user exploitation across the life-sciences.
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 3.0 Spain
http://creativecommons.org/licenses/by/3.0/es/
Attribution 3.0 Spain
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 3.0 Spain
http://creativecommons.org/licenses/by/3.0/es/
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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