ProAKAP4 as a predictor of semen freezability in Asturcon ponies

Dordas-Perpinyà, Marta|||0000-0002-5676-9009; Catalán, Jaime|||0000-0002-0878-7819; Sergeant, Nicolas|||0000-0003-1448-124X; Yánez-Ortiz, Iván|||0000-0002-3613-2481; Martínez-Hernández, J.|||0000-0003-1344-8333; Tamargo, Carolina|||0000-0002-4484-8981; Hidalgo Ordóñez, Carlos Olegario|||0000-0002-9810-1517; Briand-Amirat, Lamia|||0000-0003-1053-8077; Miró, Jordi|||0000-0002-1085-0039

ProAKAP4 as a predictor of semen freezability in Asturcon ponies

Background Cryopreservation is a valuable tool in equine reproduction, although its success remains highly variable among stallions. The proAKAP4 protein has been proposed as a molecular marker of sperm quality and cryotolerance. Aims/objectives This study aimed to evaluate the relationship between...

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Autores:	Dordas-Perpinyà, Marta\|\|\|0000-0002-5676-9009, Catalán, Jaime\|\|\|0000-0002-0878-7819, Sergeant, Nicolas\|\|\|0000-0003-1448-124X, Yánez-Ortiz, Iván\|\|\|0000-0002-3613-2481, Martínez-Hernández, J.\|\|\|0000-0003-1344-8333, Tamargo, Carolina\|\|\|0000-0002-4484-8981, Hidalgo Ordóñez, Carlos Olegario\|\|\|0000-0002-9810-1517, Briand-Amirat, Lamia\|\|\|0000-0003-1053-8077, Miró, Jordi\|\|\|0000-0002-1085-0039
Tipo de recurso:	artículo
Fecha de publicación:	2026
País:	España
Institución:	Universitat Autònoma de Barcelona
Repositorio:	Dipòsit Digital de Documents de la UAB
Idioma:	inglés
OAI Identifier:	oai:dnet:uabarcelona_::2a5e5be46278b4af797add5725e92014
Acceso en línea:	https://ddd.uab.cat/record/328809 https://dx.doi.org/urn:doi:10.1016/j.jevs.2026.105912
Access Level:	acceso abierto
Palabra clave:	ProAKAP4 AKAP4 Stallion Spermatozoa Motility Bad freezer

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spelling	ProAKAP4 as a predictor of semen freezability in Asturcon poniesDordas-Perpinyà, Marta\|\|\|0000-0002-5676-9009Catalán, Jaime\|\|\|0000-0002-0878-7819Sergeant, Nicolas\|\|\|0000-0003-1448-124XYánez-Ortiz, Iván\|\|\|0000-0002-3613-2481Martínez-Hernández, J.\|\|\|0000-0003-1344-8333Tamargo, Carolina\|\|\|0000-0002-4484-8981Hidalgo Ordóñez, Carlos Olegario\|\|\|0000-0002-9810-1517Briand-Amirat, Lamia\|\|\|0000-0003-1053-8077Miró, Jordi\|\|\|0000-0002-1085-0039ProAKAP4AKAP4StallionSpermatozoaMotilityBad freezerBackground Cryopreservation is a valuable tool in equine reproduction, although its success remains highly variable among stallions. The proAKAP4 protein has been proposed as a molecular marker of sperm quality and cryotolerance. Aims/objectives This study aimed to evaluate the relationship between proAKAP4 concentration and sperm cryotolerance in ejaculates from Asturcon ponies, and to assess whether proAKAP4 levels in fresh semen can predict post-thaw sperm quality parameters before and after cryopreservation. Methods Twenty-four ejaculates from eight different stallions were used (three ejaculates per animal). Fresh and thawed ejaculates, as well as those frozen with BotuCRIO and FAO extender, were classified into two groups based on proAKAP4 concentration (low or high) using complete linkage hierarchical cluster analysis. Sperm quality was assessed using computer-assisted sperm analysis and flow cytometry, including evaluations of motility, viability, morphology, acrosome integrity, and plasma membrane lipid disorder. ProAKAP4 concentration was measured using a commercial ELISA kit. Results Fresh and frozen ejaculates with high proAKAP4 concentrations exhibited higher total motility, progressive motility, and sperm membrane integrity (P < 0.05) than ejaculates with lower proAKAP4 concentrations. Ejaculates frozen with BotuCRIO and FAO extender and classified as having high proAKAP4 concentrations also displayed improved (P < 0.05) total and progressive motility. BotuCRIO samples with high proAKAP4 levels showed higher (P < 0.05) plasma and acrosomal membrane integrity, and lower (P < 0.05) membrane lipid disorder compared to those with lower proAKAP4 levels. A pre-freeze ProAKAP4 concentration threshold of 70.33 ng/10 spermatozoa was identified as predictive of good freezability, and a post-thaw threshold of 40 ng/10 spermatozoa was associated with acceptable quality. Conclusion These findings confirm that proAKAP4 is a promising biomarker for semen cryotolerance and support its integration into routine semen evaluation protocols for stallion selection. 22026-01-0120262026-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/328809https://dx.doi.org/urn:doi:10.1016/j.jevs.2026.105912reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 MCIN/AEI/10.13039/501100011033Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 JDC2022-049684-Iopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:dnet:uabarcelona_::2a5e5be46278b4af797add5725e920142026-06-06T12:50:31Z
dc.title.none.fl_str_mv	ProAKAP4 as a predictor of semen freezability in Asturcon ponies
title	ProAKAP4 as a predictor of semen freezability in Asturcon ponies
spellingShingle	ProAKAP4 as a predictor of semen freezability in Asturcon ponies Dordas-Perpinyà, Marta\|\|\|0000-0002-5676-9009 ProAKAP4 AKAP4 Stallion Spermatozoa Motility Bad freezer
title_short	ProAKAP4 as a predictor of semen freezability in Asturcon ponies
title_full	ProAKAP4 as a predictor of semen freezability in Asturcon ponies
title_fullStr	ProAKAP4 as a predictor of semen freezability in Asturcon ponies
title_full_unstemmed	ProAKAP4 as a predictor of semen freezability in Asturcon ponies
title_sort	ProAKAP4 as a predictor of semen freezability in Asturcon ponies
dc.creator.none.fl_str_mv	Dordas-Perpinyà, Marta\|\|\|0000-0002-5676-9009 Catalán, Jaime\|\|\|0000-0002-0878-7819 Sergeant, Nicolas\|\|\|0000-0003-1448-124X Yánez-Ortiz, Iván\|\|\|0000-0002-3613-2481 Martínez-Hernández, J.\|\|\|0000-0003-1344-8333 Tamargo, Carolina\|\|\|0000-0002-4484-8981 Hidalgo Ordóñez, Carlos Olegario\|\|\|0000-0002-9810-1517 Briand-Amirat, Lamia\|\|\|0000-0003-1053-8077 Miró, Jordi\|\|\|0000-0002-1085-0039
author	Dordas-Perpinyà, Marta\|\|\|0000-0002-5676-9009
author_facet	Dordas-Perpinyà, Marta\|\|\|0000-0002-5676-9009 Catalán, Jaime\|\|\|0000-0002-0878-7819 Sergeant, Nicolas\|\|\|0000-0003-1448-124X Yánez-Ortiz, Iván\|\|\|0000-0002-3613-2481 Martínez-Hernández, J.\|\|\|0000-0003-1344-8333 Tamargo, Carolina\|\|\|0000-0002-4484-8981 Hidalgo Ordóñez, Carlos Olegario\|\|\|0000-0002-9810-1517 Briand-Amirat, Lamia\|\|\|0000-0003-1053-8077 Miró, Jordi\|\|\|0000-0002-1085-0039
author_role	author
author2	Catalán, Jaime\|\|\|0000-0002-0878-7819 Sergeant, Nicolas\|\|\|0000-0003-1448-124X Yánez-Ortiz, Iván\|\|\|0000-0002-3613-2481 Martínez-Hernández, J.\|\|\|0000-0003-1344-8333 Tamargo, Carolina\|\|\|0000-0002-4484-8981 Hidalgo Ordóñez, Carlos Olegario\|\|\|0000-0002-9810-1517 Briand-Amirat, Lamia\|\|\|0000-0003-1053-8077 Miró, Jordi\|\|\|0000-0002-1085-0039
author2_role	author author author author author author author author
dc.subject.none.fl_str_mv	ProAKAP4 AKAP4 Stallion Spermatozoa Motility Bad freezer
topic	ProAKAP4 AKAP4 Stallion Spermatozoa Motility Bad freezer
description	Background Cryopreservation is a valuable tool in equine reproduction, although its success remains highly variable among stallions. The proAKAP4 protein has been proposed as a molecular marker of sperm quality and cryotolerance. Aims/objectives This study aimed to evaluate the relationship between proAKAP4 concentration and sperm cryotolerance in ejaculates from Asturcon ponies, and to assess whether proAKAP4 levels in fresh semen can predict post-thaw sperm quality parameters before and after cryopreservation. Methods Twenty-four ejaculates from eight different stallions were used (three ejaculates per animal). Fresh and thawed ejaculates, as well as those frozen with BotuCRIO and FAO extender, were classified into two groups based on proAKAP4 concentration (low or high) using complete linkage hierarchical cluster analysis. Sperm quality was assessed using computer-assisted sperm analysis and flow cytometry, including evaluations of motility, viability, morphology, acrosome integrity, and plasma membrane lipid disorder. ProAKAP4 concentration was measured using a commercial ELISA kit. Results Fresh and frozen ejaculates with high proAKAP4 concentrations exhibited higher total motility, progressive motility, and sperm membrane integrity (P < 0.05) than ejaculates with lower proAKAP4 concentrations. Ejaculates frozen with BotuCRIO and FAO extender and classified as having high proAKAP4 concentrations also displayed improved (P < 0.05) total and progressive motility. BotuCRIO samples with high proAKAP4 levels showed higher (P < 0.05) plasma and acrosomal membrane integrity, and lower (P < 0.05) membrane lipid disorder compared to those with lower proAKAP4 levels. A pre-freeze ProAKAP4 concentration threshold of 70.33 ng/10 spermatozoa was identified as predictive of good freezability, and a post-thaw threshold of 40 ng/10 spermatozoa was associated with acceptable quality. Conclusion These findings confirm that proAKAP4 is a promising biomarker for semen cryotolerance and support its integration into routine semen evaluation protocols for stallion selection.
publishDate	2026
dc.date.none.fl_str_mv	2 2026-01-01 2026 2026-01-01
dc.type.none.fl_str_mv	Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://ddd.uab.cat/record/328809 https://dx.doi.org/urn:doi:10.1016/j.jevs.2026.105912
url	https://ddd.uab.cat/record/328809 https://dx.doi.org/urn:doi:10.1016/j.jevs.2026.105912
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.relation.none.fl_str_mv	Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 MCIN/AEI/10.13039/501100011033 Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 JDC2022-049684-I
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona
instname_str	Universitat Autònoma de Barcelona
reponame_str	Dipòsit Digital de Documents de la UAB
collection	Dipòsit Digital de Documents de la UAB
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repository.mail.fl_str_mv
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ProAKAP4 as a predictor of semen freezability in Asturcon ponies

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