Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study

This research was supported by the Spanish Ministry of Science, Innovation and Universities through the PID2019-103892RB-I00/AEI/10.13039/501100011033 project. The authors also thank the Generalitat de Catalunya for funding through projects 2017SGR-1165 and BASE3D 001-P-001646 (co-funded by the Euro...

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Detalhes bibliográficos
Autores: Konka, J., Espanol, M., Bosch Canals, Begoña Maria, De Oliveira, E., Ginebra, M.-P.
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12328/2848
Acesso em linha:http://hdl.handle.net/20.500.12328/2848
https://dx.doi.org/10.1016/j.mtbio.2021.100137
Access Level:acceso abierto
Palavra-chave:Fosfat de calci
Adsorció de proteïnes
Nanostructura
Intercanvi iònic
Espectroscòpia Raman
Fosfato de calcio
Absorción de proteínas
Nanoestructuras
Intercambio iónico
Espectroscopia Raman
Calcium phosphates
Protein adsorption
Nanostructure
Ion exchange
Raman spectroscopy
62
620
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spelling Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic studyKonka, J.Espanol, M.Bosch Canals, Begoña MariaDe Oliveira, E.Ginebra, M.-P.Fosfat de calciAdsorció de proteïnesNanostructuraIntercanvi iònicEspectroscòpia RamanFosfato de calcioAbsorción de proteínasNanoestructurasIntercambio iónicoEspectroscopia RamanCalcium phosphatesProtein adsorptionNanostructureIon exchangeRaman spectroscopy62620This research was supported by the Spanish Ministry of Science, Innovation and Universities through the PID2019-103892RB-I00/AEI/10.13039/501100011033 project. The authors also thank the Generalitat de Catalunya for funding through projects 2017SGR-1165 and BASE3D 001-P-001646 (co-funded by the European Regional Development Fund), as well as the ICREA Academia award of MPG, the FI-AGAUR scholarship of JK, and the Serra Hunter Program of ME.Biomimetic calcium-deficient hydroxyapatite (CDHA) as a bioactive material exhibits exceptional intrinsic osteoinductive and osteogenic properties because of its nanostructure and composition, which promote a favorable microenvironment. Its high reactivity has been hypothesized to play a relevant role in the in vivo performance, mediated by the interaction with the biological fluids, which is amplified by its high specific surface area. Paradoxically, this high reactivity is also behind the in vitro cytotoxicity of this material, especially pronounced in static conditions. The present work explores the structural and physicochemical changes that CDHA undergoes in contact with physiological fluids and to investigate its interaction with proteins. Calcium-deficient hydroxyapatite discs with different micro/nanostructures, coarse (C) and fine (F), were exposed to cell-free complete culture medium over extended periods of time: 1, 7, 14, 21, 28, and 50 days. Precipitate formation was not observed in any of the materials in contact with the physiological fluid, which would indicate that the ionic exchanges were linked to incorporation into the crystal structure of CDHA or in the hydrated layer. In fact, CDHA experienced a maturation process, with a progressive increase in crystallinity and the Ca/P ratio, accompanied by an uptake of Mg and a B-type carbonation process, with a gradual propagation into the core of the samples. However, the reactivity of biomimetic hydroxyapatite was highly dependent on the specific surface area and was amplified in nanosized needle-like crystal structures (F), whereas in coarse specimens the ionic exchanges were restricted to the surface, with low penetration in the material bulk. In addition to showing a higher protein adsorption on F substrates, the proteomics study revealed the existence of protein selectivity toward F or C microstructures, as well as the capability of CDHA, and more remarkably of F-CDHA, to concentrate specific proteins from the culture medium. Finally, a substantial improvement in the material's ability to support cell proliferation was observed after the CDHA maturation process.info:eu-repo/semantics/publishedVersionElsevier2021info:eu-repo/semantics/article17http://hdl.handle.net/20.500.12328/2848https://dx.doi.org/10.1016/j.mtbio.2021.100137reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésMaterials Today Bio12;© 2021 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/).https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12328/28482026-05-29T05:05:01Z
dc.title.none.fl_str_mv Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
title Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
spellingShingle Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
Konka, J.
Fosfat de calci
Adsorció de proteïnes
Nanostructura
Intercanvi iònic
Espectroscòpia Raman
Fosfato de calcio
Absorción de proteínas
Nanoestructuras
Intercambio iónico
Espectroscopia Raman
Calcium phosphates
Protein adsorption
Nanostructure
Ion exchange
Raman spectroscopy
62
620
title_short Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
title_full Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
title_fullStr Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
title_full_unstemmed Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
title_sort Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
dc.creator.none.fl_str_mv Konka, J.
Espanol, M.
Bosch Canals, Begoña Maria
De Oliveira, E.
Ginebra, M.-P.
author Konka, J.
author_facet Konka, J.
Espanol, M.
Bosch Canals, Begoña Maria
De Oliveira, E.
Ginebra, M.-P.
author_role author
author2 Espanol, M.
Bosch Canals, Begoña Maria
De Oliveira, E.
Ginebra, M.-P.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Fosfat de calci
Adsorció de proteïnes
Nanostructura
Intercanvi iònic
Espectroscòpia Raman
Fosfato de calcio
Absorción de proteínas
Nanoestructuras
Intercambio iónico
Espectroscopia Raman
Calcium phosphates
Protein adsorption
Nanostructure
Ion exchange
Raman spectroscopy
62
620
topic Fosfat de calci
Adsorció de proteïnes
Nanostructura
Intercanvi iònic
Espectroscòpia Raman
Fosfato de calcio
Absorción de proteínas
Nanoestructuras
Intercambio iónico
Espectroscopia Raman
Calcium phosphates
Protein adsorption
Nanostructure
Ion exchange
Raman spectroscopy
62
620
description This research was supported by the Spanish Ministry of Science, Innovation and Universities through the PID2019-103892RB-I00/AEI/10.13039/501100011033 project. The authors also thank the Generalitat de Catalunya for funding through projects 2017SGR-1165 and BASE3D 001-P-001646 (co-funded by the European Regional Development Fund), as well as the ICREA Academia award of MPG, the FI-AGAUR scholarship of JK, and the Serra Hunter Program of ME.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12328/2848
https://dx.doi.org/10.1016/j.mtbio.2021.100137
url http://hdl.handle.net/20.500.12328/2848
https://dx.doi.org/10.1016/j.mtbio.2021.100137
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Materials Today Bio
12;
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 17
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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