Green Tea catechins modulate skeletal development with effects dependent on dose, time, and structure in a Down syndrome mouse model

Altered skeletal development in Down syndrome (DS) results in a brachycephalic skull, flattened face, shorter mandibular ramus, shorter limbs, and reduced bone mineral density (BMD). Our previous study showed that low doses of green tea extract enriched in epigallocatechin-3-gallate (GTE-EGCG), admi...

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Autores: Llambrich, Sergi, González-Colom, Rubèn, Wouters, Jens, Roldan, Jorge, Salassa, Sara, Wouters, Kaat, Van Bulck, Vicky, Sharpe, James, Callaerts-Vegh, Zsuzsanna, Vande Velde, Greetje, Martínez Abadías, Neus, 1978-
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/191039
Acceso en línea:https://hdl.handle.net/2445/191039
Access Level:acceso abierto
Palabra clave:Síndrome de Down
Te
Antioxidants
Down syndrome
Tea
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spelling Green Tea catechins modulate skeletal development with effects dependent on dose, time, and structure in a Down syndrome mouse modelLlambrich, SergiGonzález-Colom, RubènWouters, JensRoldan, JorgeSalassa, SaraWouters, KaatVan Bulck, VickySharpe, JamesCallaerts-Vegh, ZsuzsannaVande Velde, GreetjeMartínez Abadías, Neus, 1978-Síndrome de DownTeAntioxidantsDown syndromeTeaAntioxidantsAltered skeletal development in Down syndrome (DS) results in a brachycephalic skull, flattened face, shorter mandibular ramus, shorter limbs, and reduced bone mineral density (BMD). Our previous study showed that low doses of green tea extract enriched in epigallocatechin-3-gallate (GTE-EGCG), administered continuously from embryonic day 9 to postnatal day 29, reduced facial dysmorphologies in the Ts65Dn (TS) mouse model of DS, but high doses could exacerbate them. Here, we extended the analyses to other skeletal structures and systematically evaluated the effects of high and low doses of GTE-EGCG treatment over postnatal development in wild-type (WT) and TS mice using in vivo µCT and geometric morphometrics. TS mice developed shorter and wider faces, skulls, and mandibles, together with shorter and narrower humerus and scapula, and reduced BMD dynamically over time. Besides facial morphology, GTE-EGCG did not rescue any other skeletal phenotype in TS treated mice. In WT mice, GTE-EGCG significantly altered the shape of the skull and mandible, reduced the length and width of the long bones, and lowered the BMD. The disparate effects of GTE-EGCG depended on the dose, developmental timepoint, and anatomical structure analyzed, emphasizing the complex nature of DS and the need to further investigate the simultaneous effects of GTE-EGCG supplementation.MDPI2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/191039Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/nu14194167Nutrients, 2022, vol. 14, num. 19, p. 4167https://doi.org/10.3390/nu14194167cc-by (c) Llambrich, Sergi et al., 2022https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1910392026-05-27T06:46:51Z
dc.title.none.fl_str_mv Green Tea catechins modulate skeletal development with effects dependent on dose, time, and structure in a Down syndrome mouse model
title Green Tea catechins modulate skeletal development with effects dependent on dose, time, and structure in a Down syndrome mouse model
spellingShingle Green Tea catechins modulate skeletal development with effects dependent on dose, time, and structure in a Down syndrome mouse model
Llambrich, Sergi
Síndrome de Down
Te
Antioxidants
Down syndrome
Tea
Antioxidants
title_short Green Tea catechins modulate skeletal development with effects dependent on dose, time, and structure in a Down syndrome mouse model
title_full Green Tea catechins modulate skeletal development with effects dependent on dose, time, and structure in a Down syndrome mouse model
title_fullStr Green Tea catechins modulate skeletal development with effects dependent on dose, time, and structure in a Down syndrome mouse model
title_full_unstemmed Green Tea catechins modulate skeletal development with effects dependent on dose, time, and structure in a Down syndrome mouse model
title_sort Green Tea catechins modulate skeletal development with effects dependent on dose, time, and structure in a Down syndrome mouse model
dc.creator.none.fl_str_mv Llambrich, Sergi
González-Colom, Rubèn
Wouters, Jens
Roldan, Jorge
Salassa, Sara
Wouters, Kaat
Van Bulck, Vicky
Sharpe, James
Callaerts-Vegh, Zsuzsanna
Vande Velde, Greetje
Martínez Abadías, Neus, 1978-
author Llambrich, Sergi
author_facet Llambrich, Sergi
González-Colom, Rubèn
Wouters, Jens
Roldan, Jorge
Salassa, Sara
Wouters, Kaat
Van Bulck, Vicky
Sharpe, James
Callaerts-Vegh, Zsuzsanna
Vande Velde, Greetje
Martínez Abadías, Neus, 1978-
author_role author
author2 González-Colom, Rubèn
Wouters, Jens
Roldan, Jorge
Salassa, Sara
Wouters, Kaat
Van Bulck, Vicky
Sharpe, James
Callaerts-Vegh, Zsuzsanna
Vande Velde, Greetje
Martínez Abadías, Neus, 1978-
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Síndrome de Down
Te
Antioxidants
Down syndrome
Tea
Antioxidants
topic Síndrome de Down
Te
Antioxidants
Down syndrome
Tea
Antioxidants
description Altered skeletal development in Down syndrome (DS) results in a brachycephalic skull, flattened face, shorter mandibular ramus, shorter limbs, and reduced bone mineral density (BMD). Our previous study showed that low doses of green tea extract enriched in epigallocatechin-3-gallate (GTE-EGCG), administered continuously from embryonic day 9 to postnatal day 29, reduced facial dysmorphologies in the Ts65Dn (TS) mouse model of DS, but high doses could exacerbate them. Here, we extended the analyses to other skeletal structures and systematically evaluated the effects of high and low doses of GTE-EGCG treatment over postnatal development in wild-type (WT) and TS mice using in vivo µCT and geometric morphometrics. TS mice developed shorter and wider faces, skulls, and mandibles, together with shorter and narrower humerus and scapula, and reduced BMD dynamically over time. Besides facial morphology, GTE-EGCG did not rescue any other skeletal phenotype in TS treated mice. In WT mice, GTE-EGCG significantly altered the shape of the skull and mandible, reduced the length and width of the long bones, and lowered the BMD. The disparate effects of GTE-EGCG depended on the dose, developmental timepoint, and anatomical structure analyzed, emphasizing the complex nature of DS and the need to further investigate the simultaneous effects of GTE-EGCG supplementation.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/191039
url https://hdl.handle.net/2445/191039
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/nu14194167
Nutrients, 2022, vol. 14, num. 19, p. 4167
https://doi.org/10.3390/nu14194167
dc.rights.none.fl_str_mv cc-by (c) Llambrich, Sergi et al., 2022
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Llambrich, Sergi et al., 2022
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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