Myeloperoxidase aggravates thoracic aortic aneurysm formation in Marfan disease.

Patients suffering from Marfan syndrome (MFS), the most prevalent inherited connective tissue disorder, face premature mortality due to dissection and rupture of thoracic aortic aneurysms. Here, we questioned whether myeloperoxidase (MPO), a leucocyte-derived enzyme with high affinity to the vessel...

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Detalles Bibliográficos
Autores: Mehrkens, Dennis, Dohr, Johannes, Nettersheim, Felix Sebastian, Ballmann, Felix, Bastigkeit, Jil, Brückner, Alexander, Geissen, Simon, De Vore, Lauren, Fitsch, Cedric, Diekmann, Chris, de la Fuente-Alonso, Andrea, Schelemei, Patrik, Picard, Felix Ruben, Kochen, Malte, Arkenberg, Per, Rappenecker, Anna, Ahdab, Maysam, Nemade, Harshal, Narayan, Suchitra, Braumann, Simon, Kreuzberg, Wiebke, Hof, Alexander, Guthoff, Henning, Mensah, Benedicta Quaye, Lechner, Sebastian, Guala, Andrea, Evangelista, Artur, Teixido-Tura, Gisela, Nistal, J Francisco, Campanero, Miguel R, Kaemmerer, Harald, Wolf, Zsuzsanna, Holdenrieder, Stefan, Groenink, Maarten, van Andel, Mitzi, Mieremet, Arnout, Pfeiler, Susanne, Gerdes, Norbert, Flögel, Ulrich, Zimmermann, Laura-Maria, Sengle, Gerhard, Eich, Marie-Lisa, Schömig-Markiefka, Birgid, Adam, Matti, Fleischmann, Bernd K, Wenzel, Daniela, Redondo, Juan Miguel, de Waard, Vivian, Klinke, Anna, Baldus, Stephan, Mollenhauer, Martin, Winkels, Holger
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/27162
Acceso en línea:https://hdl.handle.net/20.500.12105/27162
Access Level:acceso embargado
Palabra clave:Marfan syndrome
Aortic aneurysm
Inflammation
Myeloperoxidase
Endothelial cells
Descripción
Sumario:Patients suffering from Marfan syndrome (MFS), the most prevalent inherited connective tissue disorder, face premature mortality due to dissection and rupture of thoracic aortic aneurysms. Here, we questioned whether myeloperoxidase (MPO), a leucocyte-derived enzyme with high affinity to the vessel wall, contributes to aortic remodelling in MFS. Plasma MPO levels were evaluated in MFS patients and healthy controls. Thoracic aortic aneurysm formation was determined in heterozygous transgenic Fbn1C1041G/+ (MFS) mice, MPO-deficient MFS mice (MFSxMpo-/-), and MFS mice treated with an MPO inhibitor by ultrasound and histology.MFS patients exhibited increased circulating MPO levels and marked aortic MPO deposition. In MFS mice, MPO-deficiency reduced aortic elastin fragmentation and aneurysm formation. RNA sequencing revealed an inflammatory gene program in aortic endothelial cells isolated from MFS mice in comparison to WT and MFSxMpo-/- mice. This was accompanied by enhanced endothelial expression of the leucocyte adhesion molecule ICAM-1, increased leucocyte adhesion, and, consequently, leucocyte infiltration in MFS aortae. Moreover, MPO directly contributed to adverse extracellular matrix remodelling through overproduction of reactive oxygen species and subsequent vascular protein modifications leading to enhanced matrix metalloproteinase 2/9 activity. Lastly, treatment of MFS mice with the orally available MPO inhibitor AZM198 attenuated TAA formation. MPO is increased in MFS and contributes to thoracic aortic dilatation by inducing inflammatory endothelial activation, oxidative stress, and adverse extracellular matrix remodelling. Pharmacological and genetic inhibition of MPO reduced MFS-related aortic dilation in mice, highlighting MPO as a promising therapeutic target in MFS.

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