Prostate tumor OVerexpressed-1 (PTOV1) down-regulates HES1 and HEY1 notch targets genes and promotes prostate cancer progression

Background PTOV1 is an adaptor protein with functions in diverse processes, including gene transcription and protein translation, whose overexpression is associated with a higher proliferation index and tumor grade in prostate cancer (PC) and other neoplasms. Here we report its interaction with the...

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Autores: Alaña, Lide, Sesé i Faustino, Marta, Cánovas, Verónica, Punyal, Yolanda, Fernández, Yolanda, Abasolo, Ibane, Torres, Inés de, Ruiz, Cristina, Espinosa Blay, Lluís, Bigas Salvans, Anna, Ramón y Cajal Agüeras, Santiago, Fernández Ruiz, Pedro Luis, Serras Rigalt, Florenci, Corominas, Montserrat (Corominas Guiu), Thomson, Timothy M., Paciucci Barzanti, Rosanna
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2014
País:España
Recursos:Universidad de Barcelona
Repositório:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/96212
Acesso em linha:https://hdl.handle.net/2445/96212
Access Level:Acceso aberto
Palavra-chave:Càncer de pròstata
Marcadors tumorals
Prostate cancer
Tumor markers
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spelling Prostate tumor OVerexpressed-1 (PTOV1) down-regulates HES1 and HEY1 notch targets genes and promotes prostate cancer progressionAlaña, LideSesé i Faustino, MartaCánovas, VerónicaPunyal, YolandaFernández, YolandaAbasolo, IbaneTorres, Inés deRuiz, CristinaEspinosa Blay, LluísBigas Salvans, AnnaRamón y Cajal Agüeras, SantiagoFernández Ruiz, Pedro LuisSerras Rigalt, FlorenciCorominas, Montserrat (Corominas Guiu)Thomson, Timothy M.Paciucci Barzanti, RosannaCàncer de pròstataMarcadors tumoralsProstate cancerTumor markersBackground PTOV1 is an adaptor protein with functions in diverse processes, including gene transcription and protein translation, whose overexpression is associated with a higher proliferation index and tumor grade in prostate cancer (PC) and other neoplasms. Here we report its interaction with the Notch pathway and its involvement in PC progression. Methods Stable PTOV1 knockdown or overexpression were performed by lentiviral transduction. Protein interactions were analyzed by co-immunoprecipitation, pull-down and/or immunofluorescence. Endogenous gene expression was analyzed by real time RT-PCR and/or Western blotting. Exogenous promoter activities were studied by luciferase assays. Gene promoter interactions were analyzed by chromatin immunoprecipitation assays (ChIP). In vivo studies were performed in the Drosophila melanogaster wing, the SCID-Beige mouse model, and human prostate cancer tissues and metastasis. The Excel package was used for statistical analysis [...].BioMed Central2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/96212Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1186/1476-4598-13-74Molecular Cancer, 2014, vol. 13, num. 74, p. 1-16http://dx.doi.org/10.1186/1476-4598-13-74cc-by (c) Alaña, Lide et al., 2014http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/962122026-05-27T06:46:51Z
dc.title.none.fl_str_mv Prostate tumor OVerexpressed-1 (PTOV1) down-regulates HES1 and HEY1 notch targets genes and promotes prostate cancer progression
title Prostate tumor OVerexpressed-1 (PTOV1) down-regulates HES1 and HEY1 notch targets genes and promotes prostate cancer progression
spellingShingle Prostate tumor OVerexpressed-1 (PTOV1) down-regulates HES1 and HEY1 notch targets genes and promotes prostate cancer progression
Alaña, Lide
Càncer de pròstata
Marcadors tumorals
Prostate cancer
Tumor markers
title_short Prostate tumor OVerexpressed-1 (PTOV1) down-regulates HES1 and HEY1 notch targets genes and promotes prostate cancer progression
title_full Prostate tumor OVerexpressed-1 (PTOV1) down-regulates HES1 and HEY1 notch targets genes and promotes prostate cancer progression
title_fullStr Prostate tumor OVerexpressed-1 (PTOV1) down-regulates HES1 and HEY1 notch targets genes and promotes prostate cancer progression
title_full_unstemmed Prostate tumor OVerexpressed-1 (PTOV1) down-regulates HES1 and HEY1 notch targets genes and promotes prostate cancer progression
title_sort Prostate tumor OVerexpressed-1 (PTOV1) down-regulates HES1 and HEY1 notch targets genes and promotes prostate cancer progression
dc.creator.none.fl_str_mv Alaña, Lide
Sesé i Faustino, Marta
Cánovas, Verónica
Punyal, Yolanda
Fernández, Yolanda
Abasolo, Ibane
Torres, Inés de
Ruiz, Cristina
Espinosa Blay, Lluís
Bigas Salvans, Anna
Ramón y Cajal Agüeras, Santiago
Fernández Ruiz, Pedro Luis
Serras Rigalt, Florenci
Corominas, Montserrat (Corominas Guiu)
Thomson, Timothy M.
Paciucci Barzanti, Rosanna
author Alaña, Lide
author_facet Alaña, Lide
Sesé i Faustino, Marta
Cánovas, Verónica
Punyal, Yolanda
Fernández, Yolanda
Abasolo, Ibane
Torres, Inés de
Ruiz, Cristina
Espinosa Blay, Lluís
Bigas Salvans, Anna
Ramón y Cajal Agüeras, Santiago
Fernández Ruiz, Pedro Luis
Serras Rigalt, Florenci
Corominas, Montserrat (Corominas Guiu)
Thomson, Timothy M.
Paciucci Barzanti, Rosanna
author_role author
author2 Sesé i Faustino, Marta
Cánovas, Verónica
Punyal, Yolanda
Fernández, Yolanda
Abasolo, Ibane
Torres, Inés de
Ruiz, Cristina
Espinosa Blay, Lluís
Bigas Salvans, Anna
Ramón y Cajal Agüeras, Santiago
Fernández Ruiz, Pedro Luis
Serras Rigalt, Florenci
Corominas, Montserrat (Corominas Guiu)
Thomson, Timothy M.
Paciucci Barzanti, Rosanna
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Càncer de pròstata
Marcadors tumorals
Prostate cancer
Tumor markers
topic Càncer de pròstata
Marcadors tumorals
Prostate cancer
Tumor markers
description Background PTOV1 is an adaptor protein with functions in diverse processes, including gene transcription and protein translation, whose overexpression is associated with a higher proliferation index and tumor grade in prostate cancer (PC) and other neoplasms. Here we report its interaction with the Notch pathway and its involvement in PC progression. Methods Stable PTOV1 knockdown or overexpression were performed by lentiviral transduction. Protein interactions were analyzed by co-immunoprecipitation, pull-down and/or immunofluorescence. Endogenous gene expression was analyzed by real time RT-PCR and/or Western blotting. Exogenous promoter activities were studied by luciferase assays. Gene promoter interactions were analyzed by chromatin immunoprecipitation assays (ChIP). In vivo studies were performed in the Drosophila melanogaster wing, the SCID-Beige mouse model, and human prostate cancer tissues and metastasis. The Excel package was used for statistical analysis [...].
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/96212
url https://hdl.handle.net/2445/96212
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1186/1476-4598-13-74
Molecular Cancer, 2014, vol. 13, num. 74, p. 1-16
http://dx.doi.org/10.1186/1476-4598-13-74
dc.rights.none.fl_str_mv cc-by (c) Alaña, Lide et al., 2014
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Alaña, Lide et al., 2014
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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