The pioneer factor Smed-gata456-1 is required for gut cell differentiation and maintenance in planarians

How adult stem cells differentiate into different cell types remains one of the most intriguing questions in regenerative medicine. Pioneer factors are transcription factors that can bind to and open chromatin, and are among the first elements involved in cell differentiation. We used the freshwater...

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Autores: González Sastre, Alejandro, Sousa, Nidia De, Adell i Creixell, Teresa, Saló i Boix, Emili
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/120335
Acceso en línea:https://hdl.handle.net/2445/120335
Access Level:acceso abierto
Palabra clave:Regeneració (Biologia)
Cèl·lules mare
Regeneration (Biology)
Stem cells
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spelling The pioneer factor Smed-gata456-1 is required for gut cell differentiation and maintenance in planariansGonzález Sastre, AlejandroSousa, Nidia DeAdell i Creixell, TeresaSaló i Boix, EmiliRegeneració (Biologia)Cèl·lules mareRegeneration (Biology)Stem cellsHow adult stem cells differentiate into different cell types remains one of the most intriguing questions in regenerative medicine. Pioneer factors are transcription factors that can bind to and open chromatin, and are among the first elements involved in cell differentiation. We used the freshwater planarian Schmidtea mediterranea as a model system to study the role of the gata456 family of pioneer factors in gut cell differentiation during both regeneration and maintenance of the digestive system. Our findings reveal the presence of two members of the gata456 family in the Schmidtea mediterranea genome; Smed-gata456-1 and Smed-gata456-2. Our results show that Smed-gata456-1 is the only ortholog with a gut cell-related function. Smed-gata456-1 is essential for the differentiation of precursors into intestinal cells and for the survival of these differentiated cells, indicating a key role in gut regeneration and maintenance. Furthermore, tissues other than the gut appear normal following Smed-gata456-1 RNA interference (RNAi), indicating a gut-specific function. Importantly, different neoblast subtypes are unaffected by Smed-gata456-1(RNAi), suggesting that 1) Smed-gata456-1 is involved in the differentiation and maintenance, but not in the early determination, of gut cells; and 2) that the stem cell compartment is not dependent on a functional gut.University of the Basque Country Press2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/120335Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1387/ijdb.160321esInternational Journal of Developmental Biology, 2017, vol. 61, p. 53-63https://doi.org/10.1387/ijdb.160321es(c) University of the Basque Country Press, 2017info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1203352026-05-27T06:46:51Z
dc.title.none.fl_str_mv The pioneer factor Smed-gata456-1 is required for gut cell differentiation and maintenance in planarians
title The pioneer factor Smed-gata456-1 is required for gut cell differentiation and maintenance in planarians
spellingShingle The pioneer factor Smed-gata456-1 is required for gut cell differentiation and maintenance in planarians
González Sastre, Alejandro
Regeneració (Biologia)
Cèl·lules mare
Regeneration (Biology)
Stem cells
title_short The pioneer factor Smed-gata456-1 is required for gut cell differentiation and maintenance in planarians
title_full The pioneer factor Smed-gata456-1 is required for gut cell differentiation and maintenance in planarians
title_fullStr The pioneer factor Smed-gata456-1 is required for gut cell differentiation and maintenance in planarians
title_full_unstemmed The pioneer factor Smed-gata456-1 is required for gut cell differentiation and maintenance in planarians
title_sort The pioneer factor Smed-gata456-1 is required for gut cell differentiation and maintenance in planarians
dc.creator.none.fl_str_mv González Sastre, Alejandro
Sousa, Nidia De
Adell i Creixell, Teresa
Saló i Boix, Emili
author González Sastre, Alejandro
author_facet González Sastre, Alejandro
Sousa, Nidia De
Adell i Creixell, Teresa
Saló i Boix, Emili
author_role author
author2 Sousa, Nidia De
Adell i Creixell, Teresa
Saló i Boix, Emili
author2_role author
author
author
dc.subject.none.fl_str_mv Regeneració (Biologia)
Cèl·lules mare
Regeneration (Biology)
Stem cells
topic Regeneració (Biologia)
Cèl·lules mare
Regeneration (Biology)
Stem cells
description How adult stem cells differentiate into different cell types remains one of the most intriguing questions in regenerative medicine. Pioneer factors are transcription factors that can bind to and open chromatin, and are among the first elements involved in cell differentiation. We used the freshwater planarian Schmidtea mediterranea as a model system to study the role of the gata456 family of pioneer factors in gut cell differentiation during both regeneration and maintenance of the digestive system. Our findings reveal the presence of two members of the gata456 family in the Schmidtea mediterranea genome; Smed-gata456-1 and Smed-gata456-2. Our results show that Smed-gata456-1 is the only ortholog with a gut cell-related function. Smed-gata456-1 is essential for the differentiation of precursors into intestinal cells and for the survival of these differentiated cells, indicating a key role in gut regeneration and maintenance. Furthermore, tissues other than the gut appear normal following Smed-gata456-1 RNA interference (RNAi), indicating a gut-specific function. Importantly, different neoblast subtypes are unaffected by Smed-gata456-1(RNAi), suggesting that 1) Smed-gata456-1 is involved in the differentiation and maintenance, but not in the early determination, of gut cells; and 2) that the stem cell compartment is not dependent on a functional gut.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/120335
url https://hdl.handle.net/2445/120335
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1387/ijdb.160321es
International Journal of Developmental Biology, 2017, vol. 61, p. 53-63
https://doi.org/10.1387/ijdb.160321es
dc.rights.none.fl_str_mv (c) University of the Basque Country Press, 2017
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) University of the Basque Country Press, 2017
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv University of the Basque Country Press
publisher.none.fl_str_mv University of the Basque Country Press
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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