Assessment of the immunomodulatory potential of betalain- and phenolic-rich extracts from Opuntia cactus fruits

Research into natural sources of molecules that can modulate the immune system has gained significant interest in recent years, with a special emphasis on plant-based alternatives that could lead to the development of immunoceuticals. This study focused on the evaluation of the immunomodulatory pote...

Descripción completa

Detalles Bibliográficos
Autores: Parralejo-Sanz, Sara, Antunes-Ricardo, Marilena, Lobo, Gloria, Requena, Teresa, Cano, M. Pilar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/390690
Acceso en línea:http://hdl.handle.net/10261/390690
Access Level:acceso abierto
Palabra clave:Opuntia
Betalains
Phenolic compounds
Antioxidant
Antiinflammatory
Fractionation
Immunomodulation
id ES_2f5dbf1496bf3073e5a7ea654a441bf2
oai_identifier_str oai:digital.csic.es:10261/390690
network_acronym_str ES
network_name_str España
repository_id_str
spelling Assessment of the immunomodulatory potential of betalain- and phenolic-rich extracts from Opuntia cactus fruitsParralejo-Sanz, SaraAntunes-Ricardo, MarilenaLobo, GloriaRequena, TeresaCano, M. PilarOpuntiaBetalainsPhenolic compoundsAntioxidantAntiinflammatoryFractionationImmunomodulationResearch into natural sources of molecules that can modulate the immune system has gained significant interest in recent years, with a special emphasis on plant-based alternatives that could lead to the development of immunoceuticals. This study focused on the evaluation of the immunomodulatory potential of extracts obtained from three Opuntia varieties abundant in betalains and phenolic compounds: Opuntia stricta (var. dillenii) whole fruit extract (OD), Opuntia ficus-indica var. Blanca peel extract (BB) and Opuntia ficus-indica var. Colorada pulp extract (COL). All three extracts from these Opuntia varieties demonstrated significant antioxidant activity in Caco-2 and HepG2 cells and stimulated phagocytosis in RAW 264.7 macrophages. Among them, OD extract exhibited the most diverse composition in betalains and phenolic compounds, and the highest immunomodulatory potential by highly inhibiting nitric oxide (NO) production and enhancing the phagocytic activity in RAW 264.7 macrophages. Consequently, its composition was fractionated by Fast Centrifugal Partition Chromatography (FCPC) to conduct a bioguided study of the compound or groups of compounds responsible for the evaluated biological activities. A total of 12 fractions were obtained (F1-F12). Overall, fractions F3, F6, and, F7 showed greater activity in modulating cytokine production and inhibition, particularly tumoral necrosis factor (TNF)-α, and interleukins (IL-6, IL-10, and IL-1β), while the whole OD extract was more effective at reducing NO production and stimulating phagocytosis. These results underscore the potential of Opuntia cactus fruits as promising candidates for the development of natural immunomodulatory agents, widening the way for future research into their application as effective immunoceuticals.This research was funded by project PID2020-118300RB-C21 from MCIN/AEI/10.13039/501100011033 and “ERDF A way of making Europe” and co-supported by the Challenge-Based Research Funding Program I007 IOR001-C5-T1-T from the Institute for Obesity Research of Tecnológico de Monterrey. Sara Parralejo-Sanz thanks the Spanish National Research Council (CSIC) for the iMOVE grant for her PhD internationalization.Peer reviewedElsevierAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)European CommissionInstituto Tecnológico y de Estudios Superiores de MonterreyConsejo Superior de Investigaciones Científicas (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/390690reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-118300RB-C21The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.fbio.2025.106093https://doi.org/10.1016/j.fbio.2025.106093Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3906902026-05-22T06:33:51Z
dc.title.none.fl_str_mv Assessment of the immunomodulatory potential of betalain- and phenolic-rich extracts from Opuntia cactus fruits
title Assessment of the immunomodulatory potential of betalain- and phenolic-rich extracts from Opuntia cactus fruits
spellingShingle Assessment of the immunomodulatory potential of betalain- and phenolic-rich extracts from Opuntia cactus fruits
Parralejo-Sanz, Sara
Opuntia
Betalains
Phenolic compounds
Antioxidant
Antiinflammatory
Fractionation
Immunomodulation
title_short Assessment of the immunomodulatory potential of betalain- and phenolic-rich extracts from Opuntia cactus fruits
title_full Assessment of the immunomodulatory potential of betalain- and phenolic-rich extracts from Opuntia cactus fruits
title_fullStr Assessment of the immunomodulatory potential of betalain- and phenolic-rich extracts from Opuntia cactus fruits
title_full_unstemmed Assessment of the immunomodulatory potential of betalain- and phenolic-rich extracts from Opuntia cactus fruits
title_sort Assessment of the immunomodulatory potential of betalain- and phenolic-rich extracts from Opuntia cactus fruits
dc.creator.none.fl_str_mv Parralejo-Sanz, Sara
Antunes-Ricardo, Marilena
Lobo, Gloria
Requena, Teresa
Cano, M. Pilar
author Parralejo-Sanz, Sara
author_facet Parralejo-Sanz, Sara
Antunes-Ricardo, Marilena
Lobo, Gloria
Requena, Teresa
Cano, M. Pilar
author_role author
author2 Antunes-Ricardo, Marilena
Lobo, Gloria
Requena, Teresa
Cano, M. Pilar
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
Instituto Tecnológico y de Estudios Superiores de Monterrey
Consejo Superior de Investigaciones Científicas (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Opuntia
Betalains
Phenolic compounds
Antioxidant
Antiinflammatory
Fractionation
Immunomodulation
topic Opuntia
Betalains
Phenolic compounds
Antioxidant
Antiinflammatory
Fractionation
Immunomodulation
description Research into natural sources of molecules that can modulate the immune system has gained significant interest in recent years, with a special emphasis on plant-based alternatives that could lead to the development of immunoceuticals. This study focused on the evaluation of the immunomodulatory potential of extracts obtained from three Opuntia varieties abundant in betalains and phenolic compounds: Opuntia stricta (var. dillenii) whole fruit extract (OD), Opuntia ficus-indica var. Blanca peel extract (BB) and Opuntia ficus-indica var. Colorada pulp extract (COL). All three extracts from these Opuntia varieties demonstrated significant antioxidant activity in Caco-2 and HepG2 cells and stimulated phagocytosis in RAW 264.7 macrophages. Among them, OD extract exhibited the most diverse composition in betalains and phenolic compounds, and the highest immunomodulatory potential by highly inhibiting nitric oxide (NO) production and enhancing the phagocytic activity in RAW 264.7 macrophages. Consequently, its composition was fractionated by Fast Centrifugal Partition Chromatography (FCPC) to conduct a bioguided study of the compound or groups of compounds responsible for the evaluated biological activities. A total of 12 fractions were obtained (F1-F12). Overall, fractions F3, F6, and, F7 showed greater activity in modulating cytokine production and inhibition, particularly tumoral necrosis factor (TNF)-α, and interleukins (IL-6, IL-10, and IL-1β), while the whole OD extract was more effective at reducing NO production and stimulating phagocytosis. These results underscore the potential of Opuntia cactus fruits as promising candidates for the development of natural immunomodulatory agents, widening the way for future research into their application as effective immunoceuticals.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/390690
url http://hdl.handle.net/10261/390690
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-118300RB-C21
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.fbio.2025.106093
https://doi.org/10.1016/j.fbio.2025.106093
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405473215610880
score 15,81155

Similares en LA Referencia