Spread of SARS-CoV-2 in hospital areas

We performed a systematic sampling and analysis of airborne SARS-CoV-2 RNA in different hospital areas to assess viral spread. Systematic air filtration was performed in rooms with COVID-19 infected patients, in corridors adjacent to these rooms, to rooms of intensive care units, and to rooms with i...

Full description

Bibliographic Details
Authors: Grimalt, Joan O, Vilchez Rueda, Helem Haydee, Fraile-Ribot, Pablo A, Marco, Esther, Campins Rosselló, Antoni, Orfila, Jaime, van Drooge, Barend L, Fanjul, Francisco
Format: article
Publication Date:2022
Country:España
Institution:Instituto de Salud Carlos III (ISCIII)
Repository:Repisalud
Language:English
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/23524
Online Access:https://hdl.handle.net/20.500.12105/23524
Access Level:Open access
Keyword:SARS-CoV-2
Nosocomial infection
Hospital infections
Covid-19 virus disease
Aerosols
Indoor air quality
Humanos
ARN Viral
COVID-19
Hospitales
Humans
RNA, Viral
Hospitals
id ES_2f5344e8a9f121bd3c99d55f3aa10cd8
oai_identifier_str oai:repisalud.isciii.es:20.500.12105/23524
network_acronym_str ES
network_name_str España
repository_id_str
spelling Spread of SARS-CoV-2 in hospital areasGrimalt, Joan OVilchez Rueda, Helem HaydeeFraile-Ribot, Pablo AMarco, EstherCampins Rosselló, AntoniOrfila, Jaimevan Drooge, Barend LFanjul, FranciscoSARS-CoV-2Nosocomial infectionHospital infectionsCovid-19 virus diseaseAerosolsIndoor air qualityHumanosSARS-CoV-2ARN ViralCOVID-19HospitalesSARS-CoV-2HumansCOVID-19RNA, ViralHospitalsWe performed a systematic sampling and analysis of airborne SARS-CoV-2 RNA in different hospital areas to assess viral spread. Systematic air filtration was performed in rooms with COVID-19 infected patients, in corridors adjacent to these rooms, to rooms of intensive care units, and to rooms with infected and uninfected patients, and in open spaces. RNA was extracted from the filters and real-time reverse transcription polymerase chain reaction was performed using the LightMix Modular SARS-CoV-2 E-gene. The highest occurrence of RNA was found in the rooms with COVID-19 patients (mean 2600 c/m(3)) and the adjacent corridor (mean 4000 c/m(3)) which was statistically significant more exposed (p < 0.01). This difference was related to the ventilation systems. As is commonly found in many hospitals, each of the rooms had an individual air inlet and outlet, while in the corridors these devices were located at the distance of every four rooms. There was a significant transfer of viruses from the COVID-19 patients' rooms to the corridors. The airborne SARS-CoV-2 RNA in the corridors of ICUs with COVID-19 patients or care rooms of uninfected patients were ten times lower, averages 190 c/m(3) and 180 c/m(3), respectively, without presenting significant differences. In all COVID-19 ICU rooms, patients were intubated and connected to respirators that filtered all exhaled air and prevented virus release, resulting in significantly lower viral concentrations in adjacent corridors. The results show that the greatest risk of nosocomial infection may also occur in hospital areas not directly exposed to the exhaled breath of infected patients. Hospitals should evaluate the ventilation systems of all units to minimize possible contagion and, most importantly, direct monitoring of SARS-CoV-2 in the air should be carried out to prevent unexpected viral exposures.Elsevier20242024-10-0420222022-03-0120222022-03-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttps://hdl.handle.net/20.500.12105/23524reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/235242026-06-12T12:43:37Z
dc.title.none.fl_str_mv Spread of SARS-CoV-2 in hospital areas
title Spread of SARS-CoV-2 in hospital areas
spellingShingle Spread of SARS-CoV-2 in hospital areas
Grimalt, Joan O
SARS-CoV-2
Nosocomial infection
Hospital infections
Covid-19 virus disease
Aerosols
Indoor air quality
Humanos
SARS-CoV-2
ARN Viral
COVID-19
Hospitales
SARS-CoV-2
Humans
COVID-19
RNA, Viral
Hospitals
title_short Spread of SARS-CoV-2 in hospital areas
title_full Spread of SARS-CoV-2 in hospital areas
title_fullStr Spread of SARS-CoV-2 in hospital areas
title_full_unstemmed Spread of SARS-CoV-2 in hospital areas
title_sort Spread of SARS-CoV-2 in hospital areas
dc.creator.none.fl_str_mv Grimalt, Joan O
Vilchez Rueda, Helem Haydee
Fraile-Ribot, Pablo A
Marco, Esther
Campins Rosselló, Antoni
Orfila, Jaime
van Drooge, Barend L
Fanjul, Francisco
author Grimalt, Joan O
author_facet Grimalt, Joan O
Vilchez Rueda, Helem Haydee
Fraile-Ribot, Pablo A
Marco, Esther
Campins Rosselló, Antoni
Orfila, Jaime
van Drooge, Barend L
Fanjul, Francisco
author_role author
author2 Vilchez Rueda, Helem Haydee
Fraile-Ribot, Pablo A
Marco, Esther
Campins Rosselló, Antoni
Orfila, Jaime
van Drooge, Barend L
Fanjul, Francisco
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv SARS-CoV-2
Nosocomial infection
Hospital infections
Covid-19 virus disease
Aerosols
Indoor air quality
Humanos
SARS-CoV-2
ARN Viral
COVID-19
Hospitales
SARS-CoV-2
Humans
COVID-19
RNA, Viral
Hospitals
topic SARS-CoV-2
Nosocomial infection
Hospital infections
Covid-19 virus disease
Aerosols
Indoor air quality
Humanos
SARS-CoV-2
ARN Viral
COVID-19
Hospitales
SARS-CoV-2
Humans
COVID-19
RNA, Viral
Hospitals
description We performed a systematic sampling and analysis of airborne SARS-CoV-2 RNA in different hospital areas to assess viral spread. Systematic air filtration was performed in rooms with COVID-19 infected patients, in corridors adjacent to these rooms, to rooms of intensive care units, and to rooms with infected and uninfected patients, and in open spaces. RNA was extracted from the filters and real-time reverse transcription polymerase chain reaction was performed using the LightMix Modular SARS-CoV-2 E-gene. The highest occurrence of RNA was found in the rooms with COVID-19 patients (mean 2600 c/m(3)) and the adjacent corridor (mean 4000 c/m(3)) which was statistically significant more exposed (p < 0.01). This difference was related to the ventilation systems. As is commonly found in many hospitals, each of the rooms had an individual air inlet and outlet, while in the corridors these devices were located at the distance of every four rooms. There was a significant transfer of viruses from the COVID-19 patients' rooms to the corridors. The airborne SARS-CoV-2 RNA in the corridors of ICUs with COVID-19 patients or care rooms of uninfected patients were ten times lower, averages 190 c/m(3) and 180 c/m(3), respectively, without presenting significant differences. In all COVID-19 ICU rooms, patients were intubated and connected to respirators that filtered all exhaled air and prevented virus release, resulting in significantly lower viral concentrations in adjacent corridors. The results show that the greatest risk of nosocomial infection may also occur in hospital areas not directly exposed to the exhaled breath of infected patients. Hospitals should evaluate the ventilation systems of all units to minimize possible contagion and, most importantly, direct monitoring of SARS-CoV-2 in the air should be carried out to prevent unexpected viral exposures.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-03-01
2022
2022-03-01
2024
2024-10-04
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12105/23524
url https://hdl.handle.net/20.500.12105/23524
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405470213537792
score 15.811543

Similar Items