Differential NtcA responsiveness to 2-oxoglutarate underlies the diversity of C/N balance regulation in Prochlorococcus

Previous studies showed differences in the regulatory response to C/N balance in Prochlorococcus with respect to other cyanobacteria, but no information was available about its causes, or the ecological advantages conferred to thrive in oligotrophic environments. We addressed the changes in key enzy...

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Autores: Domínguez-Martín, M.A., López-Lozano, A., Clavería-Gimeno, R., Velázquez-Campoy, A., Seidel, G., Burkovski, A., Díez, J., García-Fernández, J.M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universidad de Zaragoza
Repositorio:Zaguán. Repositorio Digital de la Universidad de Zaragoza
OAI Identifier:oai:zaguan.unizar.es:65257
Acceso en línea:http://zaguan.unizar.es/record/65257
Access Level:acceso abierto
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spelling Differential NtcA responsiveness to 2-oxoglutarate underlies the diversity of C/N balance regulation in ProchlorococcusDomínguez-Martín, M.A.López-Lozano, A.Clavería-Gimeno, R.Velázquez-Campoy, A.Seidel, G.Burkovski, A.Díez, J.García-Fernández, J.M.Previous studies showed differences in the regulatory response to C/N balance in Prochlorococcus with respect to other cyanobacteria, but no information was available about its causes, or the ecological advantages conferred to thrive in oligotrophic environments. We addressed the changes in key enzymes (glutamine synthetase, isocitrate dehydrogenase) and the ntcA gene (the global nitrogen regulator) involved in C/N metabolism and its regulation, in three model Prochlorococcus strains: MED4, SS120, and MIT9313. We observed a remarkable level of diversity in their response to azaserine, a glutamate synthase inhibitor which increases the concentration of the key metabolite 2-oxoglutarate, used to sense the C/N balance by cyanobacteria. Besides, we studied the binding between the global nitrogen regulator (NtcA) and the promoter of the glnA gene in the same Prochlorococcus strains, and its dependence on the 2-oxoglutarate concentration, by using isothermal titration calorimetry, surface plasmon resonance, and electrophoretic mobility shift. Our results show a reduction in the responsiveness of NtcA to 2-oxoglutarate in Prochlorococcus, especially in the MED4 and SS120 strains. This suggests a trend to streamline the regulation of C/N metabolism in late-branching Prochlorococcus strains (MED4 and SS120), in adaptation to the rather stable conditions found in the oligotrophic ocean gyres where this microorganism is most abundant.2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://zaguan.unizar.es/record/65257reponame:Zaguán. Repositorio Digital de la Universidad de Zaragozainstname:Universidad de ZaragozaInglésinfo:eu-repo/grantAgreement/ES/FIS/PI15-00663info:eu-repo/grantAgreement/ES/MINECO/BFU2013-44767-Pinfo:eu-repo/grantAgreement/ES/MINECO/BFU2013-47064-Pinfo:eu-repo/grantAgreement/ES/MINECO/BFU2016-76227-Pinfo:eu-repo/grantAgreement/ES/MINECO/BFU2016-78232-Pinfo:eu-repo/semantics/openAccessoai:zaguan.unizar.es:652572026-05-29T13:59:51Z
dc.title.none.fl_str_mv Differential NtcA responsiveness to 2-oxoglutarate underlies the diversity of C/N balance regulation in Prochlorococcus
title Differential NtcA responsiveness to 2-oxoglutarate underlies the diversity of C/N balance regulation in Prochlorococcus
spellingShingle Differential NtcA responsiveness to 2-oxoglutarate underlies the diversity of C/N balance regulation in Prochlorococcus
Domínguez-Martín, M.A.
title_short Differential NtcA responsiveness to 2-oxoglutarate underlies the diversity of C/N balance regulation in Prochlorococcus
title_full Differential NtcA responsiveness to 2-oxoglutarate underlies the diversity of C/N balance regulation in Prochlorococcus
title_fullStr Differential NtcA responsiveness to 2-oxoglutarate underlies the diversity of C/N balance regulation in Prochlorococcus
title_full_unstemmed Differential NtcA responsiveness to 2-oxoglutarate underlies the diversity of C/N balance regulation in Prochlorococcus
title_sort Differential NtcA responsiveness to 2-oxoglutarate underlies the diversity of C/N balance regulation in Prochlorococcus
dc.creator.none.fl_str_mv Domínguez-Martín, M.A.
López-Lozano, A.
Clavería-Gimeno, R.
Velázquez-Campoy, A.
Seidel, G.
Burkovski, A.
Díez, J.
García-Fernández, J.M.
author Domínguez-Martín, M.A.
author_facet Domínguez-Martín, M.A.
López-Lozano, A.
Clavería-Gimeno, R.
Velázquez-Campoy, A.
Seidel, G.
Burkovski, A.
Díez, J.
García-Fernández, J.M.
author_role author
author2 López-Lozano, A.
Clavería-Gimeno, R.
Velázquez-Campoy, A.
Seidel, G.
Burkovski, A.
Díez, J.
García-Fernández, J.M.
author2_role author
author
author
author
author
author
author
description Previous studies showed differences in the regulatory response to C/N balance in Prochlorococcus with respect to other cyanobacteria, but no information was available about its causes, or the ecological advantages conferred to thrive in oligotrophic environments. We addressed the changes in key enzymes (glutamine synthetase, isocitrate dehydrogenase) and the ntcA gene (the global nitrogen regulator) involved in C/N metabolism and its regulation, in three model Prochlorococcus strains: MED4, SS120, and MIT9313. We observed a remarkable level of diversity in their response to azaserine, a glutamate synthase inhibitor which increases the concentration of the key metabolite 2-oxoglutarate, used to sense the C/N balance by cyanobacteria. Besides, we studied the binding between the global nitrogen regulator (NtcA) and the promoter of the glnA gene in the same Prochlorococcus strains, and its dependence on the 2-oxoglutarate concentration, by using isothermal titration calorimetry, surface plasmon resonance, and electrophoretic mobility shift. Our results show a reduction in the responsiveness of NtcA to 2-oxoglutarate in Prochlorococcus, especially in the MED4 and SS120 strains. This suggests a trend to streamline the regulation of C/N metabolism in late-branching Prochlorococcus strains (MED4 and SS120), in adaptation to the rather stable conditions found in the oligotrophic ocean gyres where this microorganism is most abundant.
publishDate 2018
dc.date.none.fl_str_mv 2018
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://zaguan.unizar.es/record/65257
url http://zaguan.unizar.es/record/65257
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/ES/MINECO/BFU2013-47064-P
info:eu-repo/grantAgreement/ES/MINECO/BFU2016-76227-P
info:eu-repo/grantAgreement/ES/MINECO/BFU2016-78232-P
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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