Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion

Porcine reproductive and respiratory syndrome (PRRS) is a viral disease defined by reproductive problems, respiratory distress and a negative impact on growth rate and general condition. Virulent PRRS virus (PRRSV) strains have emerged in the last years with evident knowledge gaps in their impact on...

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Autores: Argüello, Héctor, Rodríguez‐Gómez, Irene Magdalena, Sánchez‐Carvajal, Jose María, Pallares, Francisco José, Díaz, Iván, Cabrera‐Rubio, Raúl, Crispie, Fiona, Cotter, Paul D., Mateu, Enric, Martín‐Valls, Gerard, Carrasco, Librado, Gómez‐Laguna, Jaime
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/1188
Acceso en línea:http://hdl.handle.net/20.500.12327/1188
https://doi.org/10.1111/1751-7915.13757
Access Level:acceso abierto
Palabra clave:619
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spelling Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashionArgüello, HéctorRodríguez‐Gómez, Irene MagdalenaSánchez‐Carvajal, Jose MaríaPallares, Francisco JoséDíaz, IvánCabrera‐Rubio, RaúlCrispie, FionaCotter, Paul D.Mateu, EnricMartín‐Valls, GerardCarrasco, LibradoGómez‐Laguna, Jaime619Porcine reproductive and respiratory syndrome (PRRS) is a viral disease defined by reproductive problems, respiratory distress and a negative impact on growth rate and general condition. Virulent PRRS virus (PRRSV) strains have emerged in the last years with evident knowledge gaps in their impact on the host immune response. Thus, the present study examines the impact of acute PRRS virus (PRRSV) infection, with two strains of different virulence, on selected immune parameters and on the gut microbiota composition of infected pigs using 16S rRNA compositional sequencing. Pigs were infected with a low virulent (PRRS_3249) or a virulent (Lena) PRRSV‐1 strain and euthanized at 1, 3, 6, 8 or 13 days post‐inoculation (dpi). Faeces were collected from each animal at the necropsy time‐point. Alpha and beta diversity analyses demonstrated that infection, particularly with the Lena strain, impacted the microbiome composition from 6 dpi onwards. Taxonomic differences revealed that infected pigs had higher abundance of Treponema and Methanobrevibacter (FDR < 0.05). Differences were more considerable for Lena‐ than for PRRS_3249‐infected pigs, showing the impact of strain virulence in the intestinal changes. Lena‐infected pigs had reduced abundancies of anaerobic commensals such as Roseburia, Anaerostipes, Butyricicoccus and Prevotella (P < 0.05). The depletion of these desirable commensals was significantly correlated to infection severity measured by viraemia, clinical signs, lung lesions and immune parameters (IL‐6, IFN‐γ and Hp serum levels). Altogether, the results from this study demonstrate the indirect impact of PRRSV infection on gut microbiome composition in a strain virulence‐dependent fashion and its association with selected immune markers.info:eu-repo/semantics/publishedVersionWiley Open AccessProducció AnimalSanitat Animal2021info:eu-repo/semantics/article10http://hdl.handle.net/20.500.12327/1188https://doi.org/10.1111/1751-7915.13757reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésMicrobial BiotechnologyMINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC-2014-16735/ES/ /MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2016-76111-R/ES/Caracterización del transcriptoma del macrófago alveolar porcino en las fases tempranas de la infección in vivo con cepas del virus del PRRS-1 de distinta virulencia/EC/H2020/818368/EU/Microbiome Applications for Sustainable food systems through Technologies and EnteRprise/MASTERAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/11882026-05-29T05:05:01Z
dc.title.none.fl_str_mv Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion
title Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion
spellingShingle Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion
Argüello, Héctor
619
title_short Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion
title_full Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion
title_fullStr Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion
title_full_unstemmed Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion
title_sort Porcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashion
dc.creator.none.fl_str_mv Argüello, Héctor
Rodríguez‐Gómez, Irene Magdalena
Sánchez‐Carvajal, Jose María
Pallares, Francisco José
Díaz, Iván
Cabrera‐Rubio, Raúl
Crispie, Fiona
Cotter, Paul D.
Mateu, Enric
Martín‐Valls, Gerard
Carrasco, Librado
Gómez‐Laguna, Jaime
author Argüello, Héctor
author_facet Argüello, Héctor
Rodríguez‐Gómez, Irene Magdalena
Sánchez‐Carvajal, Jose María
Pallares, Francisco José
Díaz, Iván
Cabrera‐Rubio, Raúl
Crispie, Fiona
Cotter, Paul D.
Mateu, Enric
Martín‐Valls, Gerard
Carrasco, Librado
Gómez‐Laguna, Jaime
author_role author
author2 Rodríguez‐Gómez, Irene Magdalena
Sánchez‐Carvajal, Jose María
Pallares, Francisco José
Díaz, Iván
Cabrera‐Rubio, Raúl
Crispie, Fiona
Cotter, Paul D.
Mateu, Enric
Martín‐Valls, Gerard
Carrasco, Librado
Gómez‐Laguna, Jaime
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Sanitat Animal
dc.subject.none.fl_str_mv 619
topic 619
description Porcine reproductive and respiratory syndrome (PRRS) is a viral disease defined by reproductive problems, respiratory distress and a negative impact on growth rate and general condition. Virulent PRRS virus (PRRSV) strains have emerged in the last years with evident knowledge gaps in their impact on the host immune response. Thus, the present study examines the impact of acute PRRS virus (PRRSV) infection, with two strains of different virulence, on selected immune parameters and on the gut microbiota composition of infected pigs using 16S rRNA compositional sequencing. Pigs were infected with a low virulent (PRRS_3249) or a virulent (Lena) PRRSV‐1 strain and euthanized at 1, 3, 6, 8 or 13 days post‐inoculation (dpi). Faeces were collected from each animal at the necropsy time‐point. Alpha and beta diversity analyses demonstrated that infection, particularly with the Lena strain, impacted the microbiome composition from 6 dpi onwards. Taxonomic differences revealed that infected pigs had higher abundance of Treponema and Methanobrevibacter (FDR < 0.05). Differences were more considerable for Lena‐ than for PRRS_3249‐infected pigs, showing the impact of strain virulence in the intestinal changes. Lena‐infected pigs had reduced abundancies of anaerobic commensals such as Roseburia, Anaerostipes, Butyricicoccus and Prevotella (P < 0.05). The depletion of these desirable commensals was significantly correlated to infection severity measured by viraemia, clinical signs, lung lesions and immune parameters (IL‐6, IFN‐γ and Hp serum levels). Altogether, the results from this study demonstrate the indirect impact of PRRSV infection on gut microbiome composition in a strain virulence‐dependent fashion and its association with selected immune markers.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/1188
https://doi.org/10.1111/1751-7915.13757
url http://hdl.handle.net/20.500.12327/1188
https://doi.org/10.1111/1751-7915.13757
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Microbial Biotechnology
MINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC-2014-16735/ES/ /
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2016-76111-R/ES/Caracterización del transcriptoma del macrófago alveolar porcino en las fases tempranas de la infección in vivo con cepas del virus del PRRS-1 de distinta virulencia/
EC/H2020/818368/EU/Microbiome Applications for Sustainable food systems through Technologies and EnteRprise/MASTER
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10
dc.publisher.none.fl_str_mv Wiley Open Access
publisher.none.fl_str_mv Wiley Open Access
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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