CD8 T cell responses to an immunodominant epitope within the nonstructural protein NS1 provide wide immunoprotection against bluetongue virus in IFNAR-/- mice

The development of vaccines against bluetongue, a prevalent livestock disease, has been focused on surface antigens that induce strong neutralizing antibody responses. Because of their antigenic variability, these vaccines are usually serotype restricted. We now show that a single highly conserved n...

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Autores: Marín-López, A., Calvo Pinilla, Eva María, Barriales, Diego, Lorenzo, Gema, Brun Torres, Alejandro, Anguita, Juan, Ortego, Javier
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/290589
Acceso en línea:http://hdl.handle.net/10261/290589
Access Level:acceso abierto
Palabra clave:Bluetongue
NS1
MVA
Multiserotype
DIVA
Vaccine
CD8 T cell response
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spelling CD8 T cell responses to an immunodominant epitope within the nonstructural protein NS1 provide wide immunoprotection against bluetongue virus in IFNAR-/- miceMarín-López, A.Calvo Pinilla, Eva MaríaBarriales, DiegoLorenzo, GemaBrun Torres, AlejandroAnguita, JuanOrtego, JavierBluetongueNS1MVAMultiserotypeDIVAVaccineCD8 T cell responseThe development of vaccines against bluetongue, a prevalent livestock disease, has been focused on surface antigens that induce strong neutralizing antibody responses. Because of their antigenic variability, these vaccines are usually serotype restricted. We now show that a single highly conserved nonstructural protein, NS1, expressed in a modified vaccinia Ankara virus (MVA) vector can provide multiserotype protection in IFNAR_/_ 129 mice against bluetongue virus (BTV) that is largely dependent on CD8 T cell responses. We found that the protective antigenic capacity of NS1 resides within the N terminus of the protein and is provided in the absence of neutralizing antibodies. The protective CD8 T cell response requires the presence of a specific peptide within the N terminus of NS1, since its deletion ablates the efficacy of the vaccine formulation. These data reveal the importance of the nonstructural protein NS1 in CD8 T cell-mediated protection against multiple BTV serotypes when vectorized as a recombinant MVA vaccine.Peer reviewedAmerican Society for MicrobiologyLorenzo, Gema [0000-0003-1869-9051]Brun Torres, Alejandro [0000-0001-7865-538X]Ortego, Javier [0000-0002-4275-7277]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/290589reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2905892026-05-22T06:33:51Z
dc.title.none.fl_str_mv CD8 T cell responses to an immunodominant epitope within the nonstructural protein NS1 provide wide immunoprotection against bluetongue virus in IFNAR-/- mice
title CD8 T cell responses to an immunodominant epitope within the nonstructural protein NS1 provide wide immunoprotection against bluetongue virus in IFNAR-/- mice
spellingShingle CD8 T cell responses to an immunodominant epitope within the nonstructural protein NS1 provide wide immunoprotection against bluetongue virus in IFNAR-/- mice
Marín-López, A.
Bluetongue
NS1
MVA
Multiserotype
DIVA
Vaccine
CD8 T cell response
title_short CD8 T cell responses to an immunodominant epitope within the nonstructural protein NS1 provide wide immunoprotection against bluetongue virus in IFNAR-/- mice
title_full CD8 T cell responses to an immunodominant epitope within the nonstructural protein NS1 provide wide immunoprotection against bluetongue virus in IFNAR-/- mice
title_fullStr CD8 T cell responses to an immunodominant epitope within the nonstructural protein NS1 provide wide immunoprotection against bluetongue virus in IFNAR-/- mice
title_full_unstemmed CD8 T cell responses to an immunodominant epitope within the nonstructural protein NS1 provide wide immunoprotection against bluetongue virus in IFNAR-/- mice
title_sort CD8 T cell responses to an immunodominant epitope within the nonstructural protein NS1 provide wide immunoprotection against bluetongue virus in IFNAR-/- mice
dc.creator.none.fl_str_mv Marín-López, A.
Calvo Pinilla, Eva María
Barriales, Diego
Lorenzo, Gema
Brun Torres, Alejandro
Anguita, Juan
Ortego, Javier
author Marín-López, A.
author_facet Marín-López, A.
Calvo Pinilla, Eva María
Barriales, Diego
Lorenzo, Gema
Brun Torres, Alejandro
Anguita, Juan
Ortego, Javier
author_role author
author2 Calvo Pinilla, Eva María
Barriales, Diego
Lorenzo, Gema
Brun Torres, Alejandro
Anguita, Juan
Ortego, Javier
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Lorenzo, Gema [0000-0003-1869-9051]
Brun Torres, Alejandro [0000-0001-7865-538X]
Ortego, Javier [0000-0002-4275-7277]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bluetongue
NS1
MVA
Multiserotype
DIVA
Vaccine
CD8 T cell response
topic Bluetongue
NS1
MVA
Multiserotype
DIVA
Vaccine
CD8 T cell response
description The development of vaccines against bluetongue, a prevalent livestock disease, has been focused on surface antigens that induce strong neutralizing antibody responses. Because of their antigenic variability, these vaccines are usually serotype restricted. We now show that a single highly conserved nonstructural protein, NS1, expressed in a modified vaccinia Ankara virus (MVA) vector can provide multiserotype protection in IFNAR_/_ 129 mice against bluetongue virus (BTV) that is largely dependent on CD8 T cell responses. We found that the protective antigenic capacity of NS1 resides within the N terminus of the protein and is provided in the absence of neutralizing antibodies. The protective CD8 T cell response requires the presence of a specific peptide within the N terminus of NS1, since its deletion ablates the efficacy of the vaccine formulation. These data reveal the importance of the nonstructural protein NS1 in CD8 T cell-mediated protection against multiple BTV serotypes when vectorized as a recombinant MVA vaccine.
publishDate 2018
dc.date.none.fl_str_mv 2018
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/290589
url http://hdl.handle.net/10261/290589
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405461642477568
score 15.30478

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