A high titer antibody response against P22 protein immunocomplex is not correlated with protection in naturally tuberculosis-infected goats

Caprine livestock are significant reservoirs of the Mycobacterium tuberculosis complex (MTBC), contributing to tuberculosis (TB) transmission among animals and humans. The P22 protein immunocomplex (P22PI), derived from bovine tuberculin, shows immunostimulating capacity and is used for TB diagnosis...

Descripción completa

Detalles Bibliográficos
Autores: Ortego, Joaquín, Agulló-Ros, Irene, Roy, Álvaro, Moreno, Inmaculada, Gómez-Buendía, Alberto, Romero, Beatriz, Ferreras-Colino, Elisa, Juan, Lucía de, Domínguez, Lucas, Risalde, María Ángeles, Bezos, Javier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/376834
Acceso en línea:http://hdl.handle.net/10261/376834
Access Level:acceso abierto
Palabra clave:Tuberculosis
Goat
Immunisation
Diagnosis
Lesions
P22 protein immunocomplex
id ES_2f2fafd910408279ab3bcb0ec9c15ea9
oai_identifier_str oai:digital.csic.es:10261/376834
network_acronym_str ES
network_name_str España
repository_id_str
spelling A high titer antibody response against P22 protein immunocomplex is not correlated with protection in naturally tuberculosis-infected goatsOrtego, JoaquínAgulló-Ros, IreneRoy, ÁlvaroMoreno, InmaculadaGómez-Buendía, AlbertoRomero, BeatrizFerreras-Colino, ElisaJuan, Lucía deDomínguez, LucasRisalde, María ÁngelesBezos, JavierTuberculosisGoatImmunisationDiagnosisLesionsP22 protein immunocomplexCaprine livestock are significant reservoirs of the Mycobacterium tuberculosis complex (MTBC), contributing to tuberculosis (TB) transmission among animals and humans. The P22 protein immunocomplex (P22PI), derived from bovine tuberculin, shows immunostimulating capacity and is used for TB diagnosis. This study assessed the immunogenicity and protective efficacy of P22PI in two groups of goats: 24 naïve goats (12 immunised, 12 controls) from a TB-free herd, and 24 infected goats (12 immunised, 12 controls), referred to as pre-infected animals, from a M. bovis-infected herd. Both were exposed for 5 months to M. bovis-naturally infected goats. Reactors to single and comparative intradermal tuberculin (SIT and SCIT, respectively) tests and interferon-gamma release assay (IGRA) significantly increased (p < 0.05) in both groups 5 months' post-exposure, with no significant differences between immunised and control animals. However, immunised animals exhibited a significantly higher (p < 0.05) antibody response against P22PI. Most naïve animals (83.3%) and all pre-infected animals developed TB-compatible lesions, with extensive necrosis in the lungs and associated lymph nodes, compared to 50% and 83.3% of control animals, respectively. These findings suggest that while P22PI stimulates an intense antibody response under the conditions of the present study, it does not confer protection against TB and may exacerbate disease severity.This research was made possible by funding from ICRAD, an ERA-NET co-funded under the European Union’s Horizon 2020 research and innovation program (https://ec.europa.eu/programmes/horizon2020/en) under Grant Agreement No. 862605, and the Spanish Ministry of Science, Innovation and Universities (MCIN/AEI/10.13039/501100011033) through the project “Improving the diagnosis of tuberculosis in domestic ruminants through the use of new antigens and test platforms” (reference PCI2023-143368). Moreover, this work was supported by grants from the University of Cordoba (REF: UCO-FEDER-1381385-R). JO was supported by an FPU contract-fellowship (Formación de Profesorado Universitario) from the Ministerio de Ciencia, Innovación y Universidades (FPU18/05197). IAR is supported by the FPU grant of the Ministerio de Ciencia, Innovación y Universidades (FPU19/03969).Peer reviewedTaylor & FrancisEuropean CommissionMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Universidad de Córdoba (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/376834reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/862605info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PCI2023-143368The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1080/01652176.2024.2429851https://doi.org/10.1080/01652176.2024.2429851Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3768342026-05-22T06:33:51Z
dc.title.none.fl_str_mv A high titer antibody response against P22 protein immunocomplex is not correlated with protection in naturally tuberculosis-infected goats
title A high titer antibody response against P22 protein immunocomplex is not correlated with protection in naturally tuberculosis-infected goats
spellingShingle A high titer antibody response against P22 protein immunocomplex is not correlated with protection in naturally tuberculosis-infected goats
Ortego, Joaquín
Tuberculosis
Goat
Immunisation
Diagnosis
Lesions
P22 protein immunocomplex
title_short A high titer antibody response against P22 protein immunocomplex is not correlated with protection in naturally tuberculosis-infected goats
title_full A high titer antibody response against P22 protein immunocomplex is not correlated with protection in naturally tuberculosis-infected goats
title_fullStr A high titer antibody response against P22 protein immunocomplex is not correlated with protection in naturally tuberculosis-infected goats
title_full_unstemmed A high titer antibody response against P22 protein immunocomplex is not correlated with protection in naturally tuberculosis-infected goats
title_sort A high titer antibody response against P22 protein immunocomplex is not correlated with protection in naturally tuberculosis-infected goats
dc.creator.none.fl_str_mv Ortego, Joaquín
Agulló-Ros, Irene
Roy, Álvaro
Moreno, Inmaculada
Gómez-Buendía, Alberto
Romero, Beatriz
Ferreras-Colino, Elisa
Juan, Lucía de
Domínguez, Lucas
Risalde, María Ángeles
Bezos, Javier
author Ortego, Joaquín
author_facet Ortego, Joaquín
Agulló-Ros, Irene
Roy, Álvaro
Moreno, Inmaculada
Gómez-Buendía, Alberto
Romero, Beatriz
Ferreras-Colino, Elisa
Juan, Lucía de
Domínguez, Lucas
Risalde, María Ángeles
Bezos, Javier
author_role author
author2 Agulló-Ros, Irene
Roy, Álvaro
Moreno, Inmaculada
Gómez-Buendía, Alberto
Romero, Beatriz
Ferreras-Colino, Elisa
Juan, Lucía de
Domínguez, Lucas
Risalde, María Ángeles
Bezos, Javier
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Universidad de Córdoba (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Tuberculosis
Goat
Immunisation
Diagnosis
Lesions
P22 protein immunocomplex
topic Tuberculosis
Goat
Immunisation
Diagnosis
Lesions
P22 protein immunocomplex
description Caprine livestock are significant reservoirs of the Mycobacterium tuberculosis complex (MTBC), contributing to tuberculosis (TB) transmission among animals and humans. The P22 protein immunocomplex (P22PI), derived from bovine tuberculin, shows immunostimulating capacity and is used for TB diagnosis. This study assessed the immunogenicity and protective efficacy of P22PI in two groups of goats: 24 naïve goats (12 immunised, 12 controls) from a TB-free herd, and 24 infected goats (12 immunised, 12 controls), referred to as pre-infected animals, from a M. bovis-infected herd. Both were exposed for 5 months to M. bovis-naturally infected goats. Reactors to single and comparative intradermal tuberculin (SIT and SCIT, respectively) tests and interferon-gamma release assay (IGRA) significantly increased (p < 0.05) in both groups 5 months' post-exposure, with no significant differences between immunised and control animals. However, immunised animals exhibited a significantly higher (p < 0.05) antibody response against P22PI. Most naïve animals (83.3%) and all pre-infected animals developed TB-compatible lesions, with extensive necrosis in the lungs and associated lymph nodes, compared to 50% and 83.3% of control animals, respectively. These findings suggest that while P22PI stimulates an intense antibody response under the conditions of the present study, it does not confer protection against TB and may exacerbate disease severity.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/376834
url http://hdl.handle.net/10261/376834
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/862605
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PCI2023-143368
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1080/01652176.2024.2429851
https://doi.org/10.1080/01652176.2024.2429851
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405461177958400
score 15.811543

Similares en LA Referencia