Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation.

In murine macrophages, as a result of arginine catabolism during activation, citruline isproduced under the effect of IFN-c and LPS, and ornithine and polyamines by IL-4 and IL-10. For proliferation, arginine is required from the extracellular medium and is used for protein synthesis. During activat...

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Detalles Bibliográficos
Autores: Yeramian, Andrée, Martin, Lorena, Arpa, Luis, Bertran, Joan, 1964-, Soler Prat, Concepció, McLeod, Carol, Modolell, Manuel, Palacín Prieto, Manuel, Lloberas Cavero, Jorge, Celada Cotarelo, Antonio
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2006
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/206968
Acceso en línea:https://hdl.handle.net/2445/206968
Access Level:acceso abierto
Palabra clave:Macròfags
Proliferació cel·lular
Macrophages
Cell proliferation
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spelling Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation.Yeramian, AndréeMartin, LorenaArpa, LuisBertran, Joan, 1964-Soler Prat, ConcepcióMcLeod, CarolModolell, ManuelPalacín Prieto, ManuelLloberas Cavero, JorgeCelada Cotarelo, AntonioMacròfagsProliferació cel·lularMacrophagesCell proliferationIn murine macrophages, as a result of arginine catabolism during activation, citruline isproduced under the effect of IFN-c and LPS, and ornithine and polyamines by IL-4 and IL-10. For proliferation, arginine is required from the extracellular medium and is used for protein synthesis. During activation, most arginine (>95% in 6 h) was metabolized, while under proliferation only half was incorporated into proteins. Under basal conditions, this amino acid was preferentially transported by y+L activity. During activation, arginine transport increased drastically (4–5-fold) through y+ cationic amino acid transporter (CAT) activity. By contrast, M-CSF induced only a modest increase in uptake (0.5-fold). The increase in arginine transport during activation, but not proliferation, was mediated by the SLC7A2/Cat2 gene. SLC7A1/Cat1 is constitutively expressed, and is not modified by proliferating or activating agents.M-CSF-dependent proliferation was not affected in the macrophages of SLC7A2 knockout mice; however, these cells showed a drastic reduction in the production of citruline or ornithine and polyamines during activation. The data show that a large increase in a specific transport system (CAT2) is necessary for activation-induced arginine metabolism, while arginine is in excess for the requirements of proliferation and a modest increase in transport occurs.Wiley-VCH2024202420062024info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion11 p.application/pdfhttps://hdl.handle.net/2445/206968Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/https://doi.org/10.1002/eji.200535694European Journal of Immunology, 2006, vol. 36, num.6, p. 1516-1526https://doi.org/https://doi.org/10.1002/eji.200535694(c) Wiley-VCH, 2006info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2069682026-05-29T05:05:01Z
dc.title.none.fl_str_mv Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation.
title Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation.
spellingShingle Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation.
Yeramian, Andrée
Macròfags
Proliferació cel·lular
Macrophages
Cell proliferation
title_short Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation.
title_full Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation.
title_fullStr Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation.
title_full_unstemmed Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation.
title_sort Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation.
dc.creator.none.fl_str_mv Yeramian, Andrée
Martin, Lorena
Arpa, Luis
Bertran, Joan, 1964-
Soler Prat, Concepció
McLeod, Carol
Modolell, Manuel
Palacín Prieto, Manuel
Lloberas Cavero, Jorge
Celada Cotarelo, Antonio
author Yeramian, Andrée
author_facet Yeramian, Andrée
Martin, Lorena
Arpa, Luis
Bertran, Joan, 1964-
Soler Prat, Concepció
McLeod, Carol
Modolell, Manuel
Palacín Prieto, Manuel
Lloberas Cavero, Jorge
Celada Cotarelo, Antonio
author_role author
author2 Martin, Lorena
Arpa, Luis
Bertran, Joan, 1964-
Soler Prat, Concepció
McLeod, Carol
Modolell, Manuel
Palacín Prieto, Manuel
Lloberas Cavero, Jorge
Celada Cotarelo, Antonio
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Macròfags
Proliferació cel·lular
Macrophages
Cell proliferation
topic Macròfags
Proliferació cel·lular
Macrophages
Cell proliferation
description In murine macrophages, as a result of arginine catabolism during activation, citruline isproduced under the effect of IFN-c and LPS, and ornithine and polyamines by IL-4 and IL-10. For proliferation, arginine is required from the extracellular medium and is used for protein synthesis. During activation, most arginine (>95% in 6 h) was metabolized, while under proliferation only half was incorporated into proteins. Under basal conditions, this amino acid was preferentially transported by y+L activity. During activation, arginine transport increased drastically (4–5-fold) through y+ cationic amino acid transporter (CAT) activity. By contrast, M-CSF induced only a modest increase in uptake (0.5-fold). The increase in arginine transport during activation, but not proliferation, was mediated by the SLC7A2/Cat2 gene. SLC7A1/Cat1 is constitutively expressed, and is not modified by proliferating or activating agents.M-CSF-dependent proliferation was not affected in the macrophages of SLC7A2 knockout mice; however, these cells showed a drastic reduction in the production of citruline or ornithine and polyamines during activation. The data show that a large increase in a specific transport system (CAT2) is necessary for activation-induced arginine metabolism, while arginine is in excess for the requirements of proliferation and a modest increase in transport occurs.
publishDate 2006
dc.date.none.fl_str_mv 2006
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/206968
url https://hdl.handle.net/2445/206968
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/https://doi.org/10.1002/eji.200535694
European Journal of Immunology, 2006, vol. 36, num.6, p. 1516-1526
https://doi.org/https://doi.org/10.1002/eji.200535694
dc.rights.none.fl_str_mv (c) Wiley-VCH, 2006
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Wiley-VCH, 2006
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
dc.publisher.none.fl_str_mv Wiley-VCH
publisher.none.fl_str_mv Wiley-VCH
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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