Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants

A natural Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) isolate from Florida shares a strikingly similar genotypic composition to that of a natural Spodoptera frugiperda MNPV (SfMNPV) isolate from Nicaragua. Both isolates comprise a high proportion of large-deletion genotypes that lack ge...

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Autores: Serrano García, Amaya, Williams, Trevor, Simón de Goñi, Oihane, López Ferber, Miguel, Caballero Murillo, Primitivo, Muñoz Labiano, Delia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/31944
Acceso en línea:https://hdl.handle.net/2454/31944
Access Level:acceso abierto
Palabra clave:Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV)
Spodoptera frugiperda MNPV (SfMNPV)
Occlusion bodies (OBs)
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repository_id_str
spelling Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutantsSerrano García, AmayaWilliams, TrevorSimón de Goñi, OihaneLópez Ferber, MiguelCaballero Murillo, PrimitivoMuñoz Labiano, DeliaSpodoptera exigua multiple nucleopolyhedrovirus (SeMNPV)Spodoptera frugiperda MNPV (SfMNPV)Occlusion bodies (OBs)A natural Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) isolate from Florida shares a strikingly similar genotypic composition to that of a natural Spodoptera frugiperda MNPV (SfMNPV) isolate from Nicaragua. Both isolates comprise a high proportion of large-deletion genotypes that lack genes that are essential for viral replication or transmission. To determine the likely origins of such genotypically similar population structures, we performed genomic and functional analyses of these genotypes. The homology of nucleotides in the deleted regions was as high as 79%, similar to those of other colinear genomic regions, although some SfMNPV genes were not present in SeMNPV. In addition, no potential consensus sequences were shared between the deletion flanking sequences. These results indicate an evolutionary mechanism that independently generates and sustains deletion mutants within each virus population. Functional analyses using different proportions of complete and deletion genotypes were performed with the two viruses in mixtures of occlusion bodies (OBs) or co-occluded virions. Ratios greater than 3:1 of complete/deletion genotypes resulted in reduced pathogenicity (expressed as median lethal dose), but there were no significant changes in the speed of kill. In contrast, OB yields increased only in the 1:1 mixture. The three phenotypic traits analyzed provide a broader picture of the functional significance of the most extensively deleted SeMNPV genotype and contribute toward the elucidation of the role of such mutants in baculovirus populations.This study received financial support from the Spanish Ministry for Science and Technology (AGL2008-05456-C03-01). A.S. received a predoctoral fellowship from the Spanish Ministry of Education and Culture.American Society for MicrobiologyNekazaritza EkoizpenaProducción AgrariaIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/31944reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglés© 2013, American Society for Microbiology. All Rights Reserved.info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/319442026-06-17T12:41:47Z
dc.title.none.fl_str_mv Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants
title Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants
spellingShingle Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants
Serrano García, Amaya
Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV)
Spodoptera frugiperda MNPV (SfMNPV)
Occlusion bodies (OBs)
title_short Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants
title_full Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants
title_fullStr Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants
title_full_unstemmed Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants
title_sort Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants
dc.creator.none.fl_str_mv Serrano García, Amaya
Williams, Trevor
Simón de Goñi, Oihane
López Ferber, Miguel
Caballero Murillo, Primitivo
Muñoz Labiano, Delia
author Serrano García, Amaya
author_facet Serrano García, Amaya
Williams, Trevor
Simón de Goñi, Oihane
López Ferber, Miguel
Caballero Murillo, Primitivo
Muñoz Labiano, Delia
author_role author
author2 Williams, Trevor
Simón de Goñi, Oihane
López Ferber, Miguel
Caballero Murillo, Primitivo
Muñoz Labiano, Delia
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Nekazaritza Ekoizpena
Producción Agraria
IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
dc.subject.none.fl_str_mv Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV)
Spodoptera frugiperda MNPV (SfMNPV)
Occlusion bodies (OBs)
topic Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV)
Spodoptera frugiperda MNPV (SfMNPV)
Occlusion bodies (OBs)
description A natural Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) isolate from Florida shares a strikingly similar genotypic composition to that of a natural Spodoptera frugiperda MNPV (SfMNPV) isolate from Nicaragua. Both isolates comprise a high proportion of large-deletion genotypes that lack genes that are essential for viral replication or transmission. To determine the likely origins of such genotypically similar population structures, we performed genomic and functional analyses of these genotypes. The homology of nucleotides in the deleted regions was as high as 79%, similar to those of other colinear genomic regions, although some SfMNPV genes were not present in SeMNPV. In addition, no potential consensus sequences were shared between the deletion flanking sequences. These results indicate an evolutionary mechanism that independently generates and sustains deletion mutants within each virus population. Functional analyses using different proportions of complete and deletion genotypes were performed with the two viruses in mixtures of occlusion bodies (OBs) or co-occluded virions. Ratios greater than 3:1 of complete/deletion genotypes resulted in reduced pathogenicity (expressed as median lethal dose), but there were no significant changes in the speed of kill. In contrast, OB yields increased only in the 1:1 mixture. The three phenotypic traits analyzed provide a broader picture of the functional significance of the most extensively deleted SeMNPV genotype and contribute toward the elucidation of the role of such mutants in baculovirus populations.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/31944
url https://hdl.handle.net/2454/31944
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv © 2013, American Society for Microbiology. All Rights Reserved.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © 2013, American Society for Microbiology. All Rights Reserved.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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