Mapping the expression of transient receptor potential channels across murine placental development

Transient receptor potential (TRP) channels play prominent roles in ion homeostasis by their ability to control cation influx. Mouse placentation is governed by the processes of trophoblast proliferation, invasion, differentiation, and fusion, all of which require calcium signaling. Although certain...

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Autores: De Clercq, K, Perez-Garcia, V, Van Bree, R, Pollastro, F, Peeraer, K, Voets, T, Vriens, J
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Centro de Investigación Principe Felipe (CIPF)
Repositorio:r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
OAI Identifier:oai:cipf.fundanetsuite.com:p3720
Acceso en línea:https://cipf.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=3720
Access Level:acceso abierto
Palabra clave:TRP channels
Placental development
Primary trophoblast cells
Trophoblast stem cells
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spelling Mapping the expression of transient receptor potential channels across murine placental developmentDe Clercq, KPerez-Garcia, VVan Bree, RPollastro, FPeeraer, KVoets, TVriens, JTRP channelsPlacental developmentPrimary trophoblast cellsTrophoblast stem cellsTransient receptor potential (TRP) channels play prominent roles in ion homeostasis by their ability to control cation influx. Mouse placentation is governed by the processes of trophoblast proliferation, invasion, differentiation, and fusion, all of which require calcium signaling. Although certain TRP channels have been shown to contribute to maternal-fetal transport of magnesium and calcium, a role for TRP channels in specific trophoblast functions has been disregarded. Using qRT-PCR and in situ hybridisation, the spatio-temporal expression pattern of TRP channels in the mouse placenta across gestation (E10.5-E18.5) was assessed. Prominent expression was observed for Trpv2, Trpm6, and Trpm7. Calcium microfluorimetry in primary trophoblast cells isolated at E14.5 of gestation further revealed the functional activity of TRPV2 and TRPM7. Finally, comparing TRP channels expression in mouse trophoblast stem cells (mTSCs) and mouse embryonic stem cells (mESC) confirmed the specific expression of TRPV2 during placental development. Moreover, TRP channel expression was similar in mTSCs compared to primary trophoblasts and validate mTSC as a model to study TRP channels in placental development. Collectivity, our results identify a specific spatio-temporal TRP channel expression pattern in trophoblasts, suggesting a possible involvement in regulating the process of placentation.BIRKHAUSER VERLAG AG2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://cipf.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=3720CMLS-Cellular and Molecular Life SciencesISSN: 1420682XISSNe: 14209071reponame:r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)instname:Centro de Investigación Principe Felipe (CIPF)Inglésinfo:eu-repo/semantics/openAccessoai:cipf.fundanetsuite.com:p37202026-06-17T11:19:47Z
dc.title.none.fl_str_mv Mapping the expression of transient receptor potential channels across murine placental development
title Mapping the expression of transient receptor potential channels across murine placental development
spellingShingle Mapping the expression of transient receptor potential channels across murine placental development
De Clercq, K
TRP channels
Placental development
Primary trophoblast cells
Trophoblast stem cells
title_short Mapping the expression of transient receptor potential channels across murine placental development
title_full Mapping the expression of transient receptor potential channels across murine placental development
title_fullStr Mapping the expression of transient receptor potential channels across murine placental development
title_full_unstemmed Mapping the expression of transient receptor potential channels across murine placental development
title_sort Mapping the expression of transient receptor potential channels across murine placental development
dc.creator.none.fl_str_mv De Clercq, K
Perez-Garcia, V
Van Bree, R
Pollastro, F
Peeraer, K
Voets, T
Vriens, J
author De Clercq, K
author_facet De Clercq, K
Perez-Garcia, V
Van Bree, R
Pollastro, F
Peeraer, K
Voets, T
Vriens, J
author_role author
author2 Perez-Garcia, V
Van Bree, R
Pollastro, F
Peeraer, K
Voets, T
Vriens, J
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv TRP channels
Placental development
Primary trophoblast cells
Trophoblast stem cells
topic TRP channels
Placental development
Primary trophoblast cells
Trophoblast stem cells
description Transient receptor potential (TRP) channels play prominent roles in ion homeostasis by their ability to control cation influx. Mouse placentation is governed by the processes of trophoblast proliferation, invasion, differentiation, and fusion, all of which require calcium signaling. Although certain TRP channels have been shown to contribute to maternal-fetal transport of magnesium and calcium, a role for TRP channels in specific trophoblast functions has been disregarded. Using qRT-PCR and in situ hybridisation, the spatio-temporal expression pattern of TRP channels in the mouse placenta across gestation (E10.5-E18.5) was assessed. Prominent expression was observed for Trpv2, Trpm6, and Trpm7. Calcium microfluorimetry in primary trophoblast cells isolated at E14.5 of gestation further revealed the functional activity of TRPV2 and TRPM7. Finally, comparing TRP channels expression in mouse trophoblast stem cells (mTSCs) and mouse embryonic stem cells (mESC) confirmed the specific expression of TRPV2 during placental development. Moreover, TRP channel expression was similar in mTSCs compared to primary trophoblasts and validate mTSC as a model to study TRP channels in placental development. Collectivity, our results identify a specific spatio-temporal TRP channel expression pattern in trophoblasts, suggesting a possible involvement in regulating the process of placentation.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://cipf.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=3720
url https://cipf.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=3720
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BIRKHAUSER VERLAG AG
publisher.none.fl_str_mv BIRKHAUSER VERLAG AG
dc.source.none.fl_str_mv CMLS-Cellular and Molecular Life Sciences
ISSN: 1420682X
ISSNe: 14209071
reponame:r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
instname:Centro de Investigación Principe Felipe (CIPF)
instname_str Centro de Investigación Principe Felipe (CIPF)
reponame_str r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
collection r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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