Fibroblast viability and phenotypic changes within glycated stiffened three-dimensional collagen matrices
Background: There is growing interest in the development of cell culture assays that enable the rigidity of the extracellular matrix to be increased. A promising approach is based on three-dimensional collagen type I matrices that are stiffened by cross-linking through non-enzymatic glycation with r...
| Autores: | , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/67530 |
| Acceso en línea: | https://hdl.handle.net/2445/67530 |
| Access Level: | acceso abierto |
| Palabra clave: | Fibroblasts Col·lagen Fibrosi pulmonar Collagen Pulmonary fibrosis |
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Fibroblast viability and phenotypic changes within glycated stiffened three-dimensional collagen matricesVicens Zygmunt, VanesaEstany, S.Colom, AdaiMontes Worboys, AnaMacahua, C.Sanabria, A. J.Llatjós, RogerEscobar Campuzano, IgnacioManresa, FedericoDorca i Sargatal, JordiNavajas Navarro, DanielAlcaraz Casademunt, JordiMolina Molina, MaríaFibroblastsCol·lagenFibrosi pulmonarFibroblastsCollagenPulmonary fibrosisBackground: There is growing interest in the development of cell culture assays that enable the rigidity of the extracellular matrix to be increased. A promising approach is based on three-dimensional collagen type I matrices that are stiffened by cross-linking through non-enzymatic glycation with reducing sugars. Methods: The present study evaluated the biomechanical changes in the non-enzymatically glycated type I collagen matrices, including collagen organization, the advanced glycation end products formation and stiffness achievement. Gels were glycated with ribose at different concentrations (0, 5, 15, 30 and 240 mM). The viability and the phenotypic changes of primary human lung fibroblasts cultured within the non-enzymatically glycated gels were also evaluated along three consecutive weeks. Statistical tests used for data analyze were Mann<br>Whitney U, Kruskal Wallis, Student's t-test, two-way ANOVA, multivariate ANOVA, linear regression test and mixed linear model. Results: Our findings indicated that the process of collagen glycation increases the stiffness of the matrices and generates advanced glycation end products in a ribose concentration-dependent manner. Furthermore, we identified optimal ribose concentrations and media conditions for cell viability and growth within the glycated matrices. The microenvironment of this collagen based three-dimensional culture induces α-smooth muscle actin and tenascin-C fibroblast protein expression. Finally, a progressive contractile phenotype cell differentiation was associated with the contraction of these gels. Conclusions: The use of non-enzymatic glycation with a low ribose concentration may provide a suitable model with a mechanic and oxidative modified environment with cell s embedded in it, which allowed cell proliferation and induced fibroblast phenotypic changes. Such culture model could be appropriate for investigations of the behavior and phenotypic changes in cells that occur during lung fibrosis as well as for testing different antifibrotic therapies in vitro.BioMed Central2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/67530Articles publicats en revistes (Ciències Fisiològiques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1186/s12931-015-0237-zRespiratory Research, 2015, vol. 16, p. 82-79http://dx.doi.org/10.1186/s12931-015-0237-zcc-by (c) Vicens-Zygmunt, V. et al., 2015http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/675302026-05-27T06:46:51Z |
| dc.title.none.fl_str_mv |
Fibroblast viability and phenotypic changes within glycated stiffened three-dimensional collagen matrices |
| title |
Fibroblast viability and phenotypic changes within glycated stiffened three-dimensional collagen matrices |
| spellingShingle |
Fibroblast viability and phenotypic changes within glycated stiffened three-dimensional collagen matrices Vicens Zygmunt, Vanesa Fibroblasts Col·lagen Fibrosi pulmonar Fibroblasts Collagen Pulmonary fibrosis |
| title_short |
Fibroblast viability and phenotypic changes within glycated stiffened three-dimensional collagen matrices |
| title_full |
Fibroblast viability and phenotypic changes within glycated stiffened three-dimensional collagen matrices |
| title_fullStr |
Fibroblast viability and phenotypic changes within glycated stiffened three-dimensional collagen matrices |
| title_full_unstemmed |
Fibroblast viability and phenotypic changes within glycated stiffened three-dimensional collagen matrices |
| title_sort |
Fibroblast viability and phenotypic changes within glycated stiffened three-dimensional collagen matrices |
| dc.creator.none.fl_str_mv |
Vicens Zygmunt, Vanesa Estany, S. Colom, Adai Montes Worboys, Ana Macahua, C. Sanabria, A. J. Llatjós, Roger Escobar Campuzano, Ignacio Manresa, Federico Dorca i Sargatal, Jordi Navajas Navarro, Daniel Alcaraz Casademunt, Jordi Molina Molina, María |
| author |
Vicens Zygmunt, Vanesa |
| author_facet |
Vicens Zygmunt, Vanesa Estany, S. Colom, Adai Montes Worboys, Ana Macahua, C. Sanabria, A. J. Llatjós, Roger Escobar Campuzano, Ignacio Manresa, Federico Dorca i Sargatal, Jordi Navajas Navarro, Daniel Alcaraz Casademunt, Jordi Molina Molina, María |
| author_role |
author |
| author2 |
Estany, S. Colom, Adai Montes Worboys, Ana Macahua, C. Sanabria, A. J. Llatjós, Roger Escobar Campuzano, Ignacio Manresa, Federico Dorca i Sargatal, Jordi Navajas Navarro, Daniel Alcaraz Casademunt, Jordi Molina Molina, María |
| author2_role |
author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Fibroblasts Col·lagen Fibrosi pulmonar Fibroblasts Collagen Pulmonary fibrosis |
| topic |
Fibroblasts Col·lagen Fibrosi pulmonar Fibroblasts Collagen Pulmonary fibrosis |
| description |
Background: There is growing interest in the development of cell culture assays that enable the rigidity of the extracellular matrix to be increased. A promising approach is based on three-dimensional collagen type I matrices that are stiffened by cross-linking through non-enzymatic glycation with reducing sugars. Methods: The present study evaluated the biomechanical changes in the non-enzymatically glycated type I collagen matrices, including collagen organization, the advanced glycation end products formation and stiffness achievement. Gels were glycated with ribose at different concentrations (0, 5, 15, 30 and 240 mM). The viability and the phenotypic changes of primary human lung fibroblasts cultured within the non-enzymatically glycated gels were also evaluated along three consecutive weeks. Statistical tests used for data analyze were Mann<br>Whitney U, Kruskal Wallis, Student's t-test, two-way ANOVA, multivariate ANOVA, linear regression test and mixed linear model. Results: Our findings indicated that the process of collagen glycation increases the stiffness of the matrices and generates advanced glycation end products in a ribose concentration-dependent manner. Furthermore, we identified optimal ribose concentrations and media conditions for cell viability and growth within the glycated matrices. The microenvironment of this collagen based three-dimensional culture induces α-smooth muscle actin and tenascin-C fibroblast protein expression. Finally, a progressive contractile phenotype cell differentiation was associated with the contraction of these gels. Conclusions: The use of non-enzymatic glycation with a low ribose concentration may provide a suitable model with a mechanic and oxidative modified environment with cell s embedded in it, which allowed cell proliferation and induced fibroblast phenotypic changes. Such culture model could be appropriate for investigations of the behavior and phenotypic changes in cells that occur during lung fibrosis as well as for testing different antifibrotic therapies in vitro. |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/67530 |
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https://hdl.handle.net/2445/67530 |
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Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: http://dx.doi.org/10.1186/s12931-015-0237-z Respiratory Research, 2015, vol. 16, p. 82-79 http://dx.doi.org/10.1186/s12931-015-0237-z |
| dc.rights.none.fl_str_mv |
cc-by (c) Vicens-Zygmunt, V. et al., 2015 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess |
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cc-by (c) Vicens-Zygmunt, V. et al., 2015 http://creativecommons.org/licenses/by/3.0/es |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
BioMed Central |
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BioMed Central |
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Articles publicats en revistes (Ciències Fisiològiques) reponame:Dipòsit Digital de la UB instname:Universidad de Barcelona |
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Universidad de Barcelona |
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Dipòsit Digital de la UB |
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Dipòsit Digital de la UB |
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