Down under the tunic: bacterial biodiversity hotspots and widespread ammonia-oxidizing archaea in coral reef ascidians

14 páginas, 3 tablas, 3 figuras.

Detalles Bibliográficos
Autores: Erwin, Patrick M., Pineda, M. C., Webster, Nicole, Turon, Xavier, López-Legentil, S.
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/94253
Acceso en línea:http://hdl.handle.net/10261/94253
Access Level:acceso abierto
Palabra clave:Coral reefs
Microbiota
Sea-squirt
Pyrosequencing
Holobiont
Thaumarchaeota
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spelling Down under the tunic: bacterial biodiversity hotspots and widespread ammonia-oxidizing archaea in coral reef ascidiansErwin, Patrick M.Pineda, M. C.Webster, NicoleTuron, XavierLópez-Legentil, S.Coral reefsMicrobiotaSea-squirtPyrosequencingHolobiontThaumarchaeota14 páginas, 3 tablas, 3 figuras.Ascidians are ecologically important components of marine ecosystems yet the ascidian microbiota remains largely unexplored beyond a few model species. We used 16S rRNA gene tag pyrosequencing to provide a comprehensive characterization of microbial symbionts in the tunic of 42 Great Barrier Reef ascidian samples representing 25 species. Results revealed high bacterial biodiversity (3 217 unique operational taxonomic units (OTU0.03) from 19 described and 14 candidate phyla) and the widespread occurrence of ammonia-oxidizing Thaumarchaeota in coral reef ascidians (24 of 25 host species). The ascidian microbiota was clearly differentiated from seawater microbial communities and included symbiont lineages shared with other invertebrate hosts as well as unique, ascidian-specific phylotypes. Several rare seawater microbes were markedly enriched (200–700 fold) in the ascidian tunic, suggesting that the rare biosphere of seawater may act as a conduit for horizontal symbiont transfer. However, most OTUs (71%) were rare and specific to single hosts and a significant correlation between host relatedness and symbiont community similarity was detected, indicating a high degree of host-specificity and potential role of vertical transmission in structuring these communities. We hypothesize that the complex ascidian microbiota revealed herein is maintained by the dynamic microenvironments within the ascidian tunic, offering optimal conditions for different metabolic pathways such as ample chemical substrate (ammonia-rich host waste) and physical habitat (high oxygen, low irradiance) for nitrification. Thus, ascidian hosts provide unique and fertile niches for diverse microorganisms and may represent an important and previously unrecognized habitat for nitrite/nitrate regeneration in coral reef ecosystems.This research was funded by the Marie Curie International Reintegration Grant FP7-PEOPLE-2010-RG 277038 (within the 7th European Community Framework Program), the Spanish Government projects CTM2010-17755 and CTM2010-22218 and the Catalan Government grant 2009 SGR-484 for Consolidated Research Groups. NSW was funded through an Australian Research Council Future Fellowship (FT1200100480).Peer reviewedNature Publishing Group201420142014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/94253reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1038/ismej.2013.188info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/942532026-05-22T06:33:51Z
dc.title.none.fl_str_mv Down under the tunic: bacterial biodiversity hotspots and widespread ammonia-oxidizing archaea in coral reef ascidians
title Down under the tunic: bacterial biodiversity hotspots and widespread ammonia-oxidizing archaea in coral reef ascidians
spellingShingle Down under the tunic: bacterial biodiversity hotspots and widespread ammonia-oxidizing archaea in coral reef ascidians
Erwin, Patrick M.
Coral reefs
Microbiota
Sea-squirt
Pyrosequencing
Holobiont
Thaumarchaeota
title_short Down under the tunic: bacterial biodiversity hotspots and widespread ammonia-oxidizing archaea in coral reef ascidians
title_full Down under the tunic: bacterial biodiversity hotspots and widespread ammonia-oxidizing archaea in coral reef ascidians
title_fullStr Down under the tunic: bacterial biodiversity hotspots and widespread ammonia-oxidizing archaea in coral reef ascidians
title_full_unstemmed Down under the tunic: bacterial biodiversity hotspots and widespread ammonia-oxidizing archaea in coral reef ascidians
title_sort Down under the tunic: bacterial biodiversity hotspots and widespread ammonia-oxidizing archaea in coral reef ascidians
dc.creator.none.fl_str_mv Erwin, Patrick M.
Pineda, M. C.
Webster, Nicole
Turon, Xavier
López-Legentil, S.
author Erwin, Patrick M.
author_facet Erwin, Patrick M.
Pineda, M. C.
Webster, Nicole
Turon, Xavier
López-Legentil, S.
author_role author
author2 Pineda, M. C.
Webster, Nicole
Turon, Xavier
López-Legentil, S.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Coral reefs
Microbiota
Sea-squirt
Pyrosequencing
Holobiont
Thaumarchaeota
topic Coral reefs
Microbiota
Sea-squirt
Pyrosequencing
Holobiont
Thaumarchaeota
description 14 páginas, 3 tablas, 3 figuras.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/94253
url http://hdl.handle.net/10261/94253
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1038/ismej.2013.188
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405436498673664
score 15,811543

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