High-throughput proteomics reveals the unicellular roots of animal phosphosignaling and cell differentiation

Cell-specific regulation of protein levels and activity is essential for the distribution of functions among multiple cell types in animals. The finding that many genes involved in these regulatory processes have a premetazoan origin raises the intriguing possibility that the mechanisms required for...

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Autores: Sebé-Pedrós, Arnau, Peña Paz, Marcia I., Capella Gutiérrez, Salvador Jesús, 1985-, Antó Subirats, Meritxell, Gabaldón Estevan, Juan Antonio, 1973-, Ruiz Trillo, Iñaki, Sabidó Aguadé, Eduard, 1981-
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2016
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/32237
Acceso en línea:http://hdl.handle.net/10230/32237
http://dx.doi.org/10.1016/j.devcel.2016.09.019
Access Level:acceso abierto
Palabra clave:Proteòmica
Diferenciació cel·lular
Evolució
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spelling High-throughput proteomics reveals the unicellular roots of animal phosphosignaling and cell differentiationSebé-Pedrós, ArnauPeña Paz, Marcia I.Capella Gutiérrez, Salvador Jesús, 1985-Antó Subirats, MeritxellGabaldón Estevan, Juan Antonio, 1973-Ruiz Trillo, IñakiSabidó Aguadé, Eduard, 1981-ProteòmicaDiferenciació cel·lularEvolucióCell-specific regulation of protein levels and activity is essential for the distribution of functions among multiple cell types in animals. The finding that many genes involved in these regulatory processes have a premetazoan origin raises the intriguing possibility that the mechanisms required for spatially regulated cell differentiation evolved prior to the appearance of animals. Here, we use high-throughput proteomics in Capsaspora owczarzaki, a close unicellular relative of animals, to characterize the dynamic proteome and phosphoproteome profiles of three temporally distinct cell types in this premetazoan species. We show that life-cycle transitions are linked to extensive proteome and phosphoproteome remodeling and that they affect key genes involved in animal multicellularity, such as transcription factors and tyrosine kinases. The observation of shared features between Capsaspora and metazoans indicates that elaborate and conserved phosphosignaling and proteome regulation supported temporal cell-type differentiation in the unicellular ancestor of animals.The CRG/UPF Proteomics Unit is part of the “Plataforma de Recursos Biomoleculares y Bioinformáticos (ProteoRed)” supported by grant PT13/0001 of Instituto de Salud Carlos III (ISCIII). A.S.-P. is supported by an EMBO Long-Term Fellowship (ALTF 841-2014). T.G. group research is funded in part by a grant from the Spanish Ministry of Economy and Competitiveness (BIO2012-37161), a grant from the Qatar National Research Fund (NPRP 5-298-3-086), a grant from the European Union FP7 FP7-PEOPLE-2013-ITN-606786, and a grant from the European Research Council under the European Union's Seventh Framework Program (FP/2007-2013)/ERC (grant agreement no. ERC-2012-StG-310325). I.R.-T. group research is supported by an Institució Catalana de Recerca i Estudis Avançats contract, a European Research Council Consolidator Grant (ERC-2012-Co-616960), and a grant (BFU-2011-23434) from Ministerio de Economía y Competitividad (MINECO). I.R.-T. also acknowledges financial support from Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement de la Generalitat de Catalunya (Project 2014 SGR 619 and Project 2014 SGR 678)Elsevier20172016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/32237http://dx.doi.org/10.1016/j.devcel.2016.09.019reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésDevelopmental Cell. 2016 Oct 24;39(2):186-97info:eu-repo/grantAgreement/ES/3PN/BIO2012-37161info:eu-repo/grantAgreement/EC/FP7/606786info:eu-repo/grantAgreement/EC/FP7/310325info:eu-repo/grantAgreement/EC/FP7/616960info:eu-repo/grantAgreement/ES/3PN/BFU2011-23434© Elsevier http://dx.doi.org/10.1016/j.devcel.2016.09.019info:eu-repo/semantics/openAccessoai:recercat.cat:10230/322372026-05-29T05:05:01Z
dc.title.none.fl_str_mv High-throughput proteomics reveals the unicellular roots of animal phosphosignaling and cell differentiation
title High-throughput proteomics reveals the unicellular roots of animal phosphosignaling and cell differentiation
spellingShingle High-throughput proteomics reveals the unicellular roots of animal phosphosignaling and cell differentiation
Sebé-Pedrós, Arnau
Proteòmica
Diferenciació cel·lular
Evolució
title_short High-throughput proteomics reveals the unicellular roots of animal phosphosignaling and cell differentiation
title_full High-throughput proteomics reveals the unicellular roots of animal phosphosignaling and cell differentiation
title_fullStr High-throughput proteomics reveals the unicellular roots of animal phosphosignaling and cell differentiation
title_full_unstemmed High-throughput proteomics reveals the unicellular roots of animal phosphosignaling and cell differentiation
title_sort High-throughput proteomics reveals the unicellular roots of animal phosphosignaling and cell differentiation
dc.creator.none.fl_str_mv Sebé-Pedrós, Arnau
Peña Paz, Marcia I.
Capella Gutiérrez, Salvador Jesús, 1985-
Antó Subirats, Meritxell
Gabaldón Estevan, Juan Antonio, 1973-
Ruiz Trillo, Iñaki
Sabidó Aguadé, Eduard, 1981-
author Sebé-Pedrós, Arnau
author_facet Sebé-Pedrós, Arnau
Peña Paz, Marcia I.
Capella Gutiérrez, Salvador Jesús, 1985-
Antó Subirats, Meritxell
Gabaldón Estevan, Juan Antonio, 1973-
Ruiz Trillo, Iñaki
Sabidó Aguadé, Eduard, 1981-
author_role author
author2 Peña Paz, Marcia I.
Capella Gutiérrez, Salvador Jesús, 1985-
Antó Subirats, Meritxell
Gabaldón Estevan, Juan Antonio, 1973-
Ruiz Trillo, Iñaki
Sabidó Aguadé, Eduard, 1981-
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Proteòmica
Diferenciació cel·lular
Evolució
topic Proteòmica
Diferenciació cel·lular
Evolució
description Cell-specific regulation of protein levels and activity is essential for the distribution of functions among multiple cell types in animals. The finding that many genes involved in these regulatory processes have a premetazoan origin raises the intriguing possibility that the mechanisms required for spatially regulated cell differentiation evolved prior to the appearance of animals. Here, we use high-throughput proteomics in Capsaspora owczarzaki, a close unicellular relative of animals, to characterize the dynamic proteome and phosphoproteome profiles of three temporally distinct cell types in this premetazoan species. We show that life-cycle transitions are linked to extensive proteome and phosphoproteome remodeling and that they affect key genes involved in animal multicellularity, such as transcription factors and tyrosine kinases. The observation of shared features between Capsaspora and metazoans indicates that elaborate and conserved phosphosignaling and proteome regulation supported temporal cell-type differentiation in the unicellular ancestor of animals.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/32237
http://dx.doi.org/10.1016/j.devcel.2016.09.019
url http://hdl.handle.net/10230/32237
http://dx.doi.org/10.1016/j.devcel.2016.09.019
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Developmental Cell. 2016 Oct 24;39(2):186-97
info:eu-repo/grantAgreement/ES/3PN/BIO2012-37161
info:eu-repo/grantAgreement/EC/FP7/606786
info:eu-repo/grantAgreement/EC/FP7/310325
info:eu-repo/grantAgreement/EC/FP7/616960
info:eu-repo/grantAgreement/ES/3PN/BFU2011-23434
dc.rights.none.fl_str_mv © Elsevier http://dx.doi.org/10.1016/j.devcel.2016.09.019
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © Elsevier http://dx.doi.org/10.1016/j.devcel.2016.09.019
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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