Auto-Adhesion Potential of Extraocular Aqp0 during Teleost Development

AQP0 water channels are the most abundant proteins expressed in the mammalian lens fiber membranes where they are essential for lens development and transparency. Unlike other aquaporin paralogs, mammalian AQP0 has a low intrinsic water permeability, but can form cell-to-cell junctions between the l...

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Detalles Bibliográficos
Autores: Chauvigné, François, Fjelldal, Per Gunnar, Cerdà, Joan, Finn, Roderick Nigel
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/1858
Acceso en línea:http://hdl.handle.net/20.500.12327/1858
https://doi.org/10.1371/journal.pone.0154592
Access Level:acceso abierto
Palabra clave:639
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spelling Auto-Adhesion Potential of Extraocular Aqp0 during Teleost DevelopmentChauvigné, FrançoisFjelldal, Per GunnarCerdà, JoanFinn, Roderick Nigel639AQP0 water channels are the most abundant proteins expressed in the mammalian lens fiber membranes where they are essential for lens development and transparency. Unlike other aquaporin paralogs, mammalian AQP0 has a low intrinsic water permeability, but can form cell-to-cell junctions between the lens fibers. It is not known whether the adhesive properties of AQP0 is a derived feature found only in mammals, or exists as a conserved ancestral trait in non-mammalian vertebrates. Here we show that a tetraploid teleost, the Atlantic salmon, expresses four Aqp0 paralogs in the developing lens, but also expresses significant levels of aqp0 mRNAs and proteins in the epithelia of the pronephros, presumptive enterocytes, gill filament and epidermis. Quantitative PCR reveals that aqp0 mRNA titres increase by three orders of magnitude between the onset of somitogenesis and pigmentation of the eye. Using in situ hybridization and specific antisera, we show that at least two of the channels (Aqp0a1, -0b1 and/or -0b2) are localized in the extraocular basolateral and apical membranes, while Aqp0a2 is lens-specific. Heterologous expression of the Aqp0 paralogs in adhesion-deficient mouse fibolast L-cells reveals that, as for human AQP0, each intact salmon channel retains cell-to-cell adhesive properties. The strongest Aqp0 interactions are auto-adhesion, suggesting that homo-octamers likely form the intercellular junctions of the developing lens and epithelial tissues. The present data are thus the first to show the adhesion potential of Aqp0 channels in a non-mammalian vertebrate, and further uncover a novel extraocular role of the channels during vertebrate development.info:eu-repo/semantics/publishedVersionPublic Library of ScienceProducció AnimalAqüicultura2016info:eu-repo/semantics/article21http://hdl.handle.net/20.500.12327/1858https://doi.org/10.1371/journal.pone.0154592reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésPLoS ONEMINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/AGL2013-41196-R/ES/Desarrollo de marcadores moleculares de calidad de gametos basados en aquaporinas para la acuicultura de peces marinos/Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/18582026-05-29T05:05:01Z
dc.title.none.fl_str_mv Auto-Adhesion Potential of Extraocular Aqp0 during Teleost Development
title Auto-Adhesion Potential of Extraocular Aqp0 during Teleost Development
spellingShingle Auto-Adhesion Potential of Extraocular Aqp0 during Teleost Development
Chauvigné, François
639
title_short Auto-Adhesion Potential of Extraocular Aqp0 during Teleost Development
title_full Auto-Adhesion Potential of Extraocular Aqp0 during Teleost Development
title_fullStr Auto-Adhesion Potential of Extraocular Aqp0 during Teleost Development
title_full_unstemmed Auto-Adhesion Potential of Extraocular Aqp0 during Teleost Development
title_sort Auto-Adhesion Potential of Extraocular Aqp0 during Teleost Development
dc.creator.none.fl_str_mv Chauvigné, François
Fjelldal, Per Gunnar
Cerdà, Joan
Finn, Roderick Nigel
author Chauvigné, François
author_facet Chauvigné, François
Fjelldal, Per Gunnar
Cerdà, Joan
Finn, Roderick Nigel
author_role author
author2 Fjelldal, Per Gunnar
Cerdà, Joan
Finn, Roderick Nigel
author2_role author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Aqüicultura
dc.subject.none.fl_str_mv 639
topic 639
description AQP0 water channels are the most abundant proteins expressed in the mammalian lens fiber membranes where they are essential for lens development and transparency. Unlike other aquaporin paralogs, mammalian AQP0 has a low intrinsic water permeability, but can form cell-to-cell junctions between the lens fibers. It is not known whether the adhesive properties of AQP0 is a derived feature found only in mammals, or exists as a conserved ancestral trait in non-mammalian vertebrates. Here we show that a tetraploid teleost, the Atlantic salmon, expresses four Aqp0 paralogs in the developing lens, but also expresses significant levels of aqp0 mRNAs and proteins in the epithelia of the pronephros, presumptive enterocytes, gill filament and epidermis. Quantitative PCR reveals that aqp0 mRNA titres increase by three orders of magnitude between the onset of somitogenesis and pigmentation of the eye. Using in situ hybridization and specific antisera, we show that at least two of the channels (Aqp0a1, -0b1 and/or -0b2) are localized in the extraocular basolateral and apical membranes, while Aqp0a2 is lens-specific. Heterologous expression of the Aqp0 paralogs in adhesion-deficient mouse fibolast L-cells reveals that, as for human AQP0, each intact salmon channel retains cell-to-cell adhesive properties. The strongest Aqp0 interactions are auto-adhesion, suggesting that homo-octamers likely form the intercellular junctions of the developing lens and epithelial tissues. The present data are thus the first to show the adhesion potential of Aqp0 channels in a non-mammalian vertebrate, and further uncover a novel extraocular role of the channels during vertebrate development.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/1858
https://doi.org/10.1371/journal.pone.0154592
url http://hdl.handle.net/20.500.12327/1858
https://doi.org/10.1371/journal.pone.0154592
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PLoS ONE
MINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/AGL2013-41196-R/ES/Desarrollo de marcadores moleculares de calidad de gametos basados en aquaporinas para la acuicultura de peces marinos/
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 21
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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