Quantitative atlas of collagen hydrogels reveals mesenchymal cancer cell traction adaptation to the matrix nanoarchitecture

Collagen-based hydrogels are commonly used in mechanobiology to mimic the extracellular matrix. A quantitative analysis of the influence of collagen concentration and properties on the structure and me- chanics of the hydrogels is essential for tailored design adjustments for specific in vitro condi...

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Authors: Blázquez Carmona, Pablo, Ruiz Mateos, Raquel, Barrasa Fano, Jorge, Shapeti, Apeksha, Martín Alfonso, José Enrique, Domínguez Abascal, Jaime, Van Oosterwyck, Hans, Reina Romo, Esther, Sanz Herrera, José A.
Format: article
Publication Date:2024
Country:España
Institution:Universidad de Huelva (UHU)
Repository:Arias Montano. Repositorio Institucional de la Universidad de Huelva
Language:English
OAI Identifier:oai:ariasmontano.uhu.es:10272/24759
Online Access:https://hdl.handle.net/10272/24759
Access Level:Open access
Keyword:Mechanobiology
Breast cancer cells
Traction force microscopy
Tumor microenvironment
Cell morphology
FIB-SEM
32 Ciencias Médicas
2302 Bioquímica
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spelling Quantitative atlas of collagen hydrogels reveals mesenchymal cancer cell traction adaptation to the matrix nanoarchitectureBlázquez Carmona, PabloRuiz Mateos, RaquelBarrasa Fano, JorgeShapeti, ApekshaMartín Alfonso, José EnriqueDomínguez Abascal, JaimeVan Oosterwyck, HansReina Romo, EstherSanz Herrera, José A.MechanobiologyBreast cancer cellsTraction force microscopyTumor microenvironmentCell morphologyFIB-SEM32 Ciencias Médicas2302 BioquímicaCollagen-based hydrogels are commonly used in mechanobiology to mimic the extracellular matrix. A quantitative analysis of the influence of collagen concentration and properties on the structure and me- chanics of the hydrogels is essential for tailored design adjustments for specific in vitro conditions. We combined focused ion beam scanning electron microscopy and rheology to provide a detailed quanti- tative atlas of the mechanical and nanoscale three-dimensional structural alterations that occur when manipulating different hydrogel’s physicochemistry. Moreover, we study the effects of such alterations on the phenotype of breast cancer cells and their mechanical interactions with the extracellular matrix. Regardless of the microenvironment’s pore size, porosity or mechanical properties, cancer cells are able to reach a stable mesenchymal-like morphology. Additionally, employing 3D traction force microscopy, a positive correlation between cellular tractions and ECM mechanics is observed up to a critical threshold, beyond which tractions plateau. This suggests that cancer cells in a stable mesenchymal state calibrate their mechanical interactions with the ECM to keep their migration and invasiveness capacities unaltered.Elsevier20242024-07-0120242024-07-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10272/24759reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelvainstname:Universidad de Huelva (UHU)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:ariasmontano.uhu.es:10272/247592026-06-02T14:58:11Z
dc.title.none.fl_str_mv Quantitative atlas of collagen hydrogels reveals mesenchymal cancer cell traction adaptation to the matrix nanoarchitecture
title Quantitative atlas of collagen hydrogels reveals mesenchymal cancer cell traction adaptation to the matrix nanoarchitecture
spellingShingle Quantitative atlas of collagen hydrogels reveals mesenchymal cancer cell traction adaptation to the matrix nanoarchitecture
Blázquez Carmona, Pablo
Mechanobiology
Breast cancer cells
Traction force microscopy
Tumor microenvironment
Cell morphology
FIB-SEM
32 Ciencias Médicas
2302 Bioquímica
title_short Quantitative atlas of collagen hydrogels reveals mesenchymal cancer cell traction adaptation to the matrix nanoarchitecture
title_full Quantitative atlas of collagen hydrogels reveals mesenchymal cancer cell traction adaptation to the matrix nanoarchitecture
title_fullStr Quantitative atlas of collagen hydrogels reveals mesenchymal cancer cell traction adaptation to the matrix nanoarchitecture
title_full_unstemmed Quantitative atlas of collagen hydrogels reveals mesenchymal cancer cell traction adaptation to the matrix nanoarchitecture
title_sort Quantitative atlas of collagen hydrogels reveals mesenchymal cancer cell traction adaptation to the matrix nanoarchitecture
dc.creator.none.fl_str_mv Blázquez Carmona, Pablo
Ruiz Mateos, Raquel
Barrasa Fano, Jorge
Shapeti, Apeksha
Martín Alfonso, José Enrique
Domínguez Abascal, Jaime
Van Oosterwyck, Hans
Reina Romo, Esther
Sanz Herrera, José A.
author Blázquez Carmona, Pablo
author_facet Blázquez Carmona, Pablo
Ruiz Mateos, Raquel
Barrasa Fano, Jorge
Shapeti, Apeksha
Martín Alfonso, José Enrique
Domínguez Abascal, Jaime
Van Oosterwyck, Hans
Reina Romo, Esther
Sanz Herrera, José A.
author_role author
author2 Ruiz Mateos, Raquel
Barrasa Fano, Jorge
Shapeti, Apeksha
Martín Alfonso, José Enrique
Domínguez Abascal, Jaime
Van Oosterwyck, Hans
Reina Romo, Esther
Sanz Herrera, José A.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Mechanobiology
Breast cancer cells
Traction force microscopy
Tumor microenvironment
Cell morphology
FIB-SEM
32 Ciencias Médicas
2302 Bioquímica
topic Mechanobiology
Breast cancer cells
Traction force microscopy
Tumor microenvironment
Cell morphology
FIB-SEM
32 Ciencias Médicas
2302 Bioquímica
description Collagen-based hydrogels are commonly used in mechanobiology to mimic the extracellular matrix. A quantitative analysis of the influence of collagen concentration and properties on the structure and me- chanics of the hydrogels is essential for tailored design adjustments for specific in vitro conditions. We combined focused ion beam scanning electron microscopy and rheology to provide a detailed quanti- tative atlas of the mechanical and nanoscale three-dimensional structural alterations that occur when manipulating different hydrogel’s physicochemistry. Moreover, we study the effects of such alterations on the phenotype of breast cancer cells and their mechanical interactions with the extracellular matrix. Regardless of the microenvironment’s pore size, porosity or mechanical properties, cancer cells are able to reach a stable mesenchymal-like morphology. Additionally, employing 3D traction force microscopy, a positive correlation between cellular tractions and ECM mechanics is observed up to a critical threshold, beyond which tractions plateau. This suggests that cancer cells in a stable mesenchymal state calibrate their mechanical interactions with the ECM to keep their migration and invasiveness capacities unaltered.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-07-01
2024
2024-07-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10272/24759
url https://hdl.handle.net/10272/24759
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelva
instname:Universidad de Huelva (UHU)
instname_str Universidad de Huelva (UHU)
reponame_str Arias Montano. Repositorio Institucional de la Universidad de Huelva
collection Arias Montano. Repositorio Institucional de la Universidad de Huelva
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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