Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity

Starch constitutes the most important carbon reserve in plants and is composed of branched amylopectin and linear amylose. The latter is synthesized exclusively by the Granule-Bound Starch Synthase (GBSS, EC 2.4.1.21). Here we report a readily reproducible, specific and highly sensitive protocol, wh...

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Detalles Bibliográficos
Autores: Albi Rodríguez, Tomás, Ortiz Marchena, M Isabel, Ruiz Pérez, Teresa, Romero Rodríguez, José María, Valverde Albacete, Federico
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/82556
Acceso en línea:https://hdl.handle.net/11441/82556
https://doi.org/10.21769/BioProtoc.1316
Access Level:acceso abierto
Palabra clave:Starch granule
Starch purification
GBSS activity
Granule-bound starch synthase
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spelling Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase ActivityAlbi Rodríguez, TomásOrtiz Marchena, M IsabelRuiz Pérez, TeresaRomero Rodríguez, José MaríaValverde Albacete, FedericoStarch granuleStarch purificationGBSS activityGranule-bound starch synthaseStarch constitutes the most important carbon reserve in plants and is composed of branched amylopectin and linear amylose. The latter is synthesized exclusively by the Granule-Bound Starch Synthase (GBSS, EC 2.4.1.21). Here we report a readily reproducible, specific and highly sensitive protocol, which includes the isolation of intact starch granules from Arabidopsis thaliana leaves and the subsequent determination of GBSS activity. We have applied this method to study GBSS activity in diurnal cycles in vegetative growth and during the photoperiodic transition to flowering in Arabidopsis (Tenorio et al., 2003; Ortiz-Marchena et al., 2014).España,MINECO CSD2007-00057, BIO2008-02292, and BIO2011-28847-C02-00España, Junta de Andalucía P06-CVI-01450 and P08-AGR-03582Bio-ProtocolBioquímica Vegetal y Biología MolecularMinisterio de Economía y Competitividad (MINECO). EspañaJunta de Andalucía2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/82556https://doi.org/10.21769/BioProtoc.1316reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésBio-protocol, 4 (23), 1-8.CSD2007-00057, BIO2008-02292, and BIO2011-28847-C02-00P06-CVI-01450 and P08-AGR-03582http://dx.doi.org/10.21769/BioProtoc.1316info:eu-repo/semantics/openAccessoai:idus.us.es:11441/825562026-06-17T12:51:07Z
dc.title.none.fl_str_mv Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity
title Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity
spellingShingle Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity
Albi Rodríguez, Tomás
Starch granule
Starch purification
GBSS activity
Granule-bound starch synthase
title_short Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity
title_full Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity
title_fullStr Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity
title_full_unstemmed Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity
title_sort Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity
dc.creator.none.fl_str_mv Albi Rodríguez, Tomás
Ortiz Marchena, M Isabel
Ruiz Pérez, Teresa
Romero Rodríguez, José María
Valverde Albacete, Federico
author Albi Rodríguez, Tomás
author_facet Albi Rodríguez, Tomás
Ortiz Marchena, M Isabel
Ruiz Pérez, Teresa
Romero Rodríguez, José María
Valverde Albacete, Federico
author_role author
author2 Ortiz Marchena, M Isabel
Ruiz Pérez, Teresa
Romero Rodríguez, José María
Valverde Albacete, Federico
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Bioquímica Vegetal y Biología Molecular
Ministerio de Economía y Competitividad (MINECO). España
Junta de Andalucía
dc.subject.none.fl_str_mv Starch granule
Starch purification
GBSS activity
Granule-bound starch synthase
topic Starch granule
Starch purification
GBSS activity
Granule-bound starch synthase
description Starch constitutes the most important carbon reserve in plants and is composed of branched amylopectin and linear amylose. The latter is synthesized exclusively by the Granule-Bound Starch Synthase (GBSS, EC 2.4.1.21). Here we report a readily reproducible, specific and highly sensitive protocol, which includes the isolation of intact starch granules from Arabidopsis thaliana leaves and the subsequent determination of GBSS activity. We have applied this method to study GBSS activity in diurnal cycles in vegetative growth and during the photoperiodic transition to flowering in Arabidopsis (Tenorio et al., 2003; Ortiz-Marchena et al., 2014).
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/82556
https://doi.org/10.21769/BioProtoc.1316
url https://hdl.handle.net/11441/82556
https://doi.org/10.21769/BioProtoc.1316
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Bio-protocol, 4 (23), 1-8.
CSD2007-00057, BIO2008-02292, and BIO2011-28847-C02-00
P06-CVI-01450 and P08-AGR-03582
http://dx.doi.org/10.21769/BioProtoc.1316
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bio-Protocol
publisher.none.fl_str_mv Bio-Protocol
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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