Chitosan increases tomato root colonization by Pochonia chlamydosporia and their combination reduces Root-Knot nematode damage

The use of biological control agents could be a non-chemical alternative for management of Meloidogyne spp. [root-knot nematodes (RKN)], the most damaging plant-parasitic nematodes for horticultural crops worldwide. Pochonia chlamydosporia is a fungal parasite of RKN eggs that can colonize endophyti...

Descripción completa

Detalles Bibliográficos
Autores: Escudero Benito, Nuria|||0000-0002-9131-0674, Lopez Moya, Federico, Ghahremani, Zahra, Zavala Gonzalez, Ernesto A, Alaguero Cordovilla, Aurora, Ros Ibañez, Caridad, Lacasa, Alfredo, Sorribas Royo, Francisco Javier|||0000-0001-7465-7353, López Llorca, Luis Vicente
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/116341
Acceso en línea:https://hdl.handle.net/2117/116341
https://dx.doi.org/10.3389/fpls.2017.01415
Access Level:acceso abierto
Palabra clave:Root-knot nematodes
endophytic colonization
nematophagous fungi
root-knot nematodes
suppressive soil
Solanum lycopersicum
Agrotech
Nematodes fitoparàsits
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura
id ES_2e9e6e013dcf7af4db5ff63acbaf5da0
oai_identifier_str oai:upcommons.upc.edu:2117/116341
network_acronym_str ES
network_name_str España
repository_id_str
spelling Chitosan increases tomato root colonization by Pochonia chlamydosporia and their combination reduces Root-Knot nematode damageEscudero Benito, Nuria|||0000-0002-9131-0674Lopez Moya, FedericoGhahremani, ZahraZavala Gonzalez, Ernesto AAlaguero Cordovilla, AuroraRos Ibañez, CaridadLacasa, AlfredoSorribas Royo, Francisco Javier|||0000-0001-7465-7353López Llorca, Luis VicenteRoot-knot nematodesendophytic colonizationnematophagous fungiroot-knot nematodessuppressive soilSolanum lycopersicumAgrotechNematodes fitoparàsitsÀrees temàtiques de la UPC::Enginyeria agroalimentària::AgriculturaThe use of biological control agents could be a non-chemical alternative for management of Meloidogyne spp. [root-knot nematodes (RKN)], the most damaging plant-parasitic nematodes for horticultural crops worldwide. Pochonia chlamydosporia is a fungal parasite of RKN eggs that can colonize endophytically roots of several cultivated plant species, but in field applications the fungus shows a low persistence and efficiency in RKN management. The combined use of P. chlamydosporia with an enhancer could help its ability to develop in soil and colonize roots, thereby increasing its efficiency against nematodes. Previous work has shown that chitosan enhances P. chlamydosporia sporulation and production of extracellular enzymes, as well as nematode egg parasitism in laboratory bioassays. This work shows that chitosan at low concentrations (up to 0.1 mg ml-1) do not affect the viability and germination of P. chlamydosporia chlamydospores and improves mycelial growth respect to treatments without chitosan. Tomato plants irrigated with chitosan (same dose limit) increased root weight and length after 30 days. Chitosan irrigation increased dry shoot and fresh root weight of tomato plants inoculated with Meloidogyne javanica, root length when they were inoculated with P. chlamydosporia, and dry shoot weight of plants inoculated with both P. chlamydosporia and M. javanica. Chitosan irrigation significantly enhanced root colonization by P. chlamydosporia, but neither nematode infection per plant nor fungal egg parasitism was affected. Tomato plants cultivated in a mid-suppressive (29.3 ± 4.7% RKN egg infection) non-sterilized clay loam soil and irrigated with chitosan had enhanced shoot growth, reduced RKN multiplication, and disease severity. Chitosan irrigation in a highly suppressive (73.7 ± 2.6% RKN egg infection) sterilized-sandy loam soil reduced RKN multiplication in tomato. However, chitosan did not affect disease severity or plant growth irrespective of soil sterilization. Chitosan, at an adequate dose, can be a potential tool for sustainable management of RKN.20172017-09-0120182018-04-16journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/116341https://dx.doi.org/10.3389/fpls.2017.0141528919898reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)InglésengMinisterio de Economía y Competitividad http://doi.org/10.13039/501100003329 AGL2013-49040-C2-1-R EFECTO DE LA RESISTENCIA DE GENES R Y LA INDUCIDA POR HONGOS ENDOFITOS EN LA EPIDEMIOLOGIA DE MELOIDOGYNE Y LA PRODUCCION Y CALIDAD DE LA COSECHA EN SOLANACEAS-CUCURBITACEASMinisterio de Economía y Competitividad http://doi.org/10.13039/501100003329 AGL2015-66833-R MODULACION DE INTERACCIONES RIZOSFERICAS PARA PROMOVER CRECIMIENTO, COSECHA Y DEFENSAS FRENTE A PATOGENOS RADICULARES DE CULTIVOSopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/1163412026-05-27T15:37:01Z
dc.title.none.fl_str_mv Chitosan increases tomato root colonization by Pochonia chlamydosporia and their combination reduces Root-Knot nematode damage
title Chitosan increases tomato root colonization by Pochonia chlamydosporia and their combination reduces Root-Knot nematode damage
spellingShingle Chitosan increases tomato root colonization by Pochonia chlamydosporia and their combination reduces Root-Knot nematode damage
Escudero Benito, Nuria|||0000-0002-9131-0674
Root-knot nematodes
endophytic colonization
nematophagous fungi
root-knot nematodes
suppressive soil
Solanum lycopersicum
Agrotech
Nematodes fitoparàsits
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura
title_short Chitosan increases tomato root colonization by Pochonia chlamydosporia and their combination reduces Root-Knot nematode damage
title_full Chitosan increases tomato root colonization by Pochonia chlamydosporia and their combination reduces Root-Knot nematode damage
title_fullStr Chitosan increases tomato root colonization by Pochonia chlamydosporia and their combination reduces Root-Knot nematode damage
title_full_unstemmed Chitosan increases tomato root colonization by Pochonia chlamydosporia and their combination reduces Root-Knot nematode damage
title_sort Chitosan increases tomato root colonization by Pochonia chlamydosporia and their combination reduces Root-Knot nematode damage
dc.creator.none.fl_str_mv Escudero Benito, Nuria|||0000-0002-9131-0674
Lopez Moya, Federico
Ghahremani, Zahra
Zavala Gonzalez, Ernesto A
Alaguero Cordovilla, Aurora
Ros Ibañez, Caridad
Lacasa, Alfredo
Sorribas Royo, Francisco Javier|||0000-0001-7465-7353
López Llorca, Luis Vicente
author Escudero Benito, Nuria|||0000-0002-9131-0674
author_facet Escudero Benito, Nuria|||0000-0002-9131-0674
Lopez Moya, Federico
Ghahremani, Zahra
Zavala Gonzalez, Ernesto A
Alaguero Cordovilla, Aurora
Ros Ibañez, Caridad
Lacasa, Alfredo
Sorribas Royo, Francisco Javier|||0000-0001-7465-7353
López Llorca, Luis Vicente
author_role author
author2 Lopez Moya, Federico
Ghahremani, Zahra
Zavala Gonzalez, Ernesto A
Alaguero Cordovilla, Aurora
Ros Ibañez, Caridad
Lacasa, Alfredo
Sorribas Royo, Francisco Javier|||0000-0001-7465-7353
López Llorca, Luis Vicente
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Root-knot nematodes
endophytic colonization
nematophagous fungi
root-knot nematodes
suppressive soil
Solanum lycopersicum
Agrotech
Nematodes fitoparàsits
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura
topic Root-knot nematodes
endophytic colonization
nematophagous fungi
root-knot nematodes
suppressive soil
Solanum lycopersicum
Agrotech
Nematodes fitoparàsits
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura
description The use of biological control agents could be a non-chemical alternative for management of Meloidogyne spp. [root-knot nematodes (RKN)], the most damaging plant-parasitic nematodes for horticultural crops worldwide. Pochonia chlamydosporia is a fungal parasite of RKN eggs that can colonize endophytically roots of several cultivated plant species, but in field applications the fungus shows a low persistence and efficiency in RKN management. The combined use of P. chlamydosporia with an enhancer could help its ability to develop in soil and colonize roots, thereby increasing its efficiency against nematodes. Previous work has shown that chitosan enhances P. chlamydosporia sporulation and production of extracellular enzymes, as well as nematode egg parasitism in laboratory bioassays. This work shows that chitosan at low concentrations (up to 0.1 mg ml-1) do not affect the viability and germination of P. chlamydosporia chlamydospores and improves mycelial growth respect to treatments without chitosan. Tomato plants irrigated with chitosan (same dose limit) increased root weight and length after 30 days. Chitosan irrigation increased dry shoot and fresh root weight of tomato plants inoculated with Meloidogyne javanica, root length when they were inoculated with P. chlamydosporia, and dry shoot weight of plants inoculated with both P. chlamydosporia and M. javanica. Chitosan irrigation significantly enhanced root colonization by P. chlamydosporia, but neither nematode infection per plant nor fungal egg parasitism was affected. Tomato plants cultivated in a mid-suppressive (29.3 ± 4.7% RKN egg infection) non-sterilized clay loam soil and irrigated with chitosan had enhanced shoot growth, reduced RKN multiplication, and disease severity. Chitosan irrigation in a highly suppressive (73.7 ± 2.6% RKN egg infection) sterilized-sandy loam soil reduced RKN multiplication in tomato. However, chitosan did not affect disease severity or plant growth irrespective of soil sterilization. Chitosan, at an adequate dose, can be a potential tool for sustainable management of RKN.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-09-01
2018
2018-04-16
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/116341
https://dx.doi.org/10.3389/fpls.2017.01415
28919898
url https://hdl.handle.net/2117/116341
https://dx.doi.org/10.3389/fpls.2017.01415
identifier_str_mv 28919898
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://doi.org/10.13039/501100003329 AGL2013-49040-C2-1-R EFECTO DE LA RESISTENCIA DE GENES R Y LA INDUCIDA POR HONGOS ENDOFITOS EN LA EPIDEMIOLOGIA DE MELOIDOGYNE Y LA PRODUCCION Y CALIDAD DE LA COSECHA EN SOLANACEAS-CUCURBITACEAS
Ministerio de Economía y Competitividad http://doi.org/10.13039/501100003329 AGL2015-66833-R MODULACION DE INTERACCIONES RIZOSFERICAS PARA PROMOVER CRECIMIENTO, COSECHA Y DEFENSAS FRENTE A PATOGENOS RADICULARES DE CULTIVOS
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405416965799936
score 15,300724

Similares en LA Referencia