A polyphasic method for the characterization of epiphytic diatoms growing on Gelidium corneum
[EN] Epiphytic diatoms associated with marine macroalgae play vital ecological roles in nutrient cycling and primary production, yet their study remains limited due to the lack of standardized methodologies. This study focuses on diatom communities growing on Gelidium corneum, a key red alga in the...
| Autores: | , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Recursos: | Universidad de León |
| Repositorio: | BULERIA. Repositorio Institucional de la Universidad de León |
| OAI Identifier: | oai:buleria.unileon.es:10612/25033 |
| Acesso em linha: | https://www.sciencedirect.com/science/article/pii/S2215016125000366?via%3Dihub https://hdl.handle.net/10612/25033 |
| Access Level: | acceso abierto |
| Palavra-chave: | Biología Protocol Bentos Epiphytism Macroalgae Bacillariophyta RBCL marker Metabarcoding |
| Resumo: | [EN] Epiphytic diatoms associated with marine macroalgae play vital ecological roles in nutrient cycling and primary production, yet their study remains limited due to the lack of standardized methodologies. This study focuses on diatom communities growing on Gelidium corneum, a key red alga in the Cantabrian coast (Spain). Samples were collected from two depths along the northern coast of Spain and processed using both morphological and molecular approaches. Morphological analysis involved diatom frustule preparation using hydrogen peroxide digestion, acid treatments, and permanent slide mounting, enabling identification through light microscopy. Molecular analysis employed DNA extraction and rbcL marker-based metabarcoding, allowing detailed taxonomic characterization. Results highlight the efficacy of combining morphological and molecular techniques to overcome the limitations of either approach individually. By standardizing procedures, we enhance the reproducibility and comparability of studies focused on diatom epiphytes. Our results highlight the ecological significance of diatom-macroalgal interactions and provide a framework for future investigations into these essential but underexplored communities. |
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