Batch studies on the biodegradation of paracetamol and 1,4-hydroquinone by novel bacterial strains isolated from extreme environmental samples and the identification of candidate catabolic genes

18 páginas.- 6 figuras.- 1 tabla.- 100 referencias.-The online version contains supplementary material available at https://doi.org/10.1007/s13201-024-02264-6

Detalhes bibliográficos
Autores: Lara Moreno, Alba, El-Sayed, Fatma, Cox, Cymon J., Costa, Maria Clara, Carlier, Jorge D.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/370426
Acesso em linha:http://hdl.handle.net/10261/370426
Access Level:acceso abierto
Palavra-chave:Bioremediation
Aerobic biodegradation
Wastewater treatment plants
Mycolicibacterium aubagnense HPB1.1
Emerging contaminant
Biodegrading genes
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spelling Batch studies on the biodegradation of paracetamol and 1,4-hydroquinone by novel bacterial strains isolated from extreme environmental samples and the identification of candidate catabolic genesLara Moreno, AlbaEl-Sayed, FatmaCox, Cymon J.Costa, Maria ClaraCarlier, Jorge D.BioremediationAerobic biodegradationWastewater treatment plantsMycolicibacterium aubagnense HPB1.1Emerging contaminantBiodegrading genes18 páginas.- 6 figuras.- 1 tabla.- 100 referencias.-The online version contains supplementary material available at https://doi.org/10.1007/s13201-024-02264-6The emerging pollutant paracetamol (APAP) is one of the most prescribed drugs worldwide. In addition, APAP and its main metabolites, namely, 4-aminophenol (4-AP), hydroquinone (H2Q), benzoquinone (BQ), and 2,5-dihydroxy-1,4-benzoquinone (2,5-OH-BQ), among others, are frequently detected in wastewater treatment plants (WWTPs) influents, effluents, and the environment. Thus, continuous release into the environment, especially aquatic environments, is a source of general concern. Six APAP-degrading bacterial strains were isolated from two mine samples from the Iberian Pyrite Belt (Lousal and Poderosa mines). Mycolicibacterium aubagnense HPB1.1, which was isolated using enrichment cultures from the Poderosa mine sample in the presence of H2Q as the sole carbon source, also showed APAP biodegrading capabilities. Pure cultures of this strain degraded 34.3 mg L−1 of APAP in 5 days and 9.4 mg L−1 of H2Q in 4 days. Interestingly, BQ and 2,5-OH-BQ were detected as metabolites resulting from H2Q abiotic degradation, but these compounds were removed in the strain’s cultures. Furthermore, M. aubagnense HPB1.1 whole-genome was sequenced, and its encoded proteins were aligned with enzymes of APAP-degrading bacteria recovered from databases and literature aiming to identify candidate catabolic genes. Putative amidases, deaminases, hydroxylases, and dioxygenases, responsible for the degradation of APAP by the HPB1.1 strain, were identified by similarity, corroborating its ability to transform APAP and its intermediate metabolite H2Q into less toxic metabolic compounds due to their capacity to break the aromatic ring of these molecules.This work is financed by Portuguese national funds from FCT—Fundação para a Ciência e a Tecnologia, I.P., within the scope of the project PTDC/CTA-AMB/7782/2020.Alba Lara-Moreno acknowledges at University of Seville for her Margarita Salas grant funded by the European Union’s Next-Generation EU. Fatma El-Sayed acknowledges the European commission and the Erasmus Mundus program for fnancing their Master of Science degree. This work was carried out in part using the Structural and Analytical Chemistry Platform of CCMAR, for spectrophotometry and HPLC analysis and the Molecular Biology Platform for 16S rRNA sequencing. This study received Portuguese national funds from FCT—Foundation for Science and Technology through projects UIDB/04326/2020, UIDP/04326/2020 and LA/P/0101/2020 to Maria C. Costa and Cymon J. Cox.Peer reviewedSpringerFundação para a Ciência e a Tecnologia (Portugal)Universidad de SevillaEuropean CommissionLara Moreno, Alba [0000-0002-5741-7662]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/370426reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1007/s13201-024-02264-6Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3704262026-05-22T06:33:51Z
dc.title.none.fl_str_mv Batch studies on the biodegradation of paracetamol and 1,4-hydroquinone by novel bacterial strains isolated from extreme environmental samples and the identification of candidate catabolic genes
title Batch studies on the biodegradation of paracetamol and 1,4-hydroquinone by novel bacterial strains isolated from extreme environmental samples and the identification of candidate catabolic genes
spellingShingle Batch studies on the biodegradation of paracetamol and 1,4-hydroquinone by novel bacterial strains isolated from extreme environmental samples and the identification of candidate catabolic genes
Lara Moreno, Alba
Bioremediation
Aerobic biodegradation
Wastewater treatment plants
Mycolicibacterium aubagnense HPB1.1
Emerging contaminant
Biodegrading genes
title_short Batch studies on the biodegradation of paracetamol and 1,4-hydroquinone by novel bacterial strains isolated from extreme environmental samples and the identification of candidate catabolic genes
title_full Batch studies on the biodegradation of paracetamol and 1,4-hydroquinone by novel bacterial strains isolated from extreme environmental samples and the identification of candidate catabolic genes
title_fullStr Batch studies on the biodegradation of paracetamol and 1,4-hydroquinone by novel bacterial strains isolated from extreme environmental samples and the identification of candidate catabolic genes
title_full_unstemmed Batch studies on the biodegradation of paracetamol and 1,4-hydroquinone by novel bacterial strains isolated from extreme environmental samples and the identification of candidate catabolic genes
title_sort Batch studies on the biodegradation of paracetamol and 1,4-hydroquinone by novel bacterial strains isolated from extreme environmental samples and the identification of candidate catabolic genes
dc.creator.none.fl_str_mv Lara Moreno, Alba
El-Sayed, Fatma
Cox, Cymon J.
Costa, Maria Clara
Carlier, Jorge D.
author Lara Moreno, Alba
author_facet Lara Moreno, Alba
El-Sayed, Fatma
Cox, Cymon J.
Costa, Maria Clara
Carlier, Jorge D.
author_role author
author2 El-Sayed, Fatma
Cox, Cymon J.
Costa, Maria Clara
Carlier, Jorge D.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Fundação para a Ciência e a Tecnologia (Portugal)
Universidad de Sevilla
European Commission
Lara Moreno, Alba [0000-0002-5741-7662]
dc.subject.none.fl_str_mv Bioremediation
Aerobic biodegradation
Wastewater treatment plants
Mycolicibacterium aubagnense HPB1.1
Emerging contaminant
Biodegrading genes
topic Bioremediation
Aerobic biodegradation
Wastewater treatment plants
Mycolicibacterium aubagnense HPB1.1
Emerging contaminant
Biodegrading genes
description 18 páginas.- 6 figuras.- 1 tabla.- 100 referencias.-The online version contains supplementary material available at https://doi.org/10.1007/s13201-024-02264-6
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/370426
url http://hdl.handle.net/10261/370426
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1007/s13201-024-02264-6
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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