Oxidative stress is evident in erythrocytes as well as plasma in patients undergoing heart surgery involving cardiopulmonary bypass.
Objective : The aim of this study was to analyse the level and progression of oxidative stress, in both plasma and erythrocytes, during heart surgery involving cardiopulmonary bypass. Materials and Methods : Twenty two patients undergoing cardiac surgery and considered to present a high/severe level...
| Autores: | , , , , , |
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| Formato: | artículo |
| Fecha de publicación: | 2003 |
| País: | España |
| Recursos: | Universidad Francisco de Vitoria |
| Repositorio: | DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria |
| Idioma: | inglés |
| OAI Identifier: | oai:ddfv.ufv.es:10641/5688 |
| Acesso em linha: | https://hdl.handle.net/10641/5688 |
| Access Level: | acceso abierto |
| Palavra-chave: | Oxidative Stress Cardiopulmonary Bypass Antioxidants Erythrocyte Plasma |
| Resumo: | Objective : The aim of this study was to analyse the level and progression of oxidative stress, in both plasma and erythrocytes, during heart surgery involving cardiopulmonary bypass. Materials and Methods : Twenty two patients undergoing cardiac surgery and considered to present a high/severe level of surgical risk were selected. We took five blood samples at different times during the cardiac surgery and analysed TBARS, f -tocopherol, coenzyme Q and retinol in plasma and TBARS (baseline levels and induced by Fe 2+ -ascorbate oxidation), f -tocopherol, coenzyme Q and catalase, superoxide dismutase and gluthatione peroxidase activity in erythrocytes. Results : Plasma results shown a decrease in both f -tocopherol and retinol concentration after starting CPB with respect to the reference level (13.6 - 1.5 nmol ml m 1 vs. 22.0 - 3.0 nmol ml m 1 and 1.2 - 0.1 nmol ml m 1 vs. 1.8 - 0.2 nmol ml m 1 , respectively ( p <0.05)). In comparison, in erythrocytes, all antioxidants, both enzymatic and non-enzymatic, increased in activity or concentration after starting CPB. Erythrocyte TBARS, both baseline levels and induced levels, followed a similar pattern, with an increase after starting CPB with respect to the reference level ( 3.9 - 0.6 nmol mg m 1 of protein vs. 2.3 - 0.2 nmol mg m 1 of protein and 10.6 - 0.8 nmol mg m 1 of protein vs. 6.7 - 0.6 nmol mg m 1 of protein, respectively ( p <0.05)). Conclusion : These results reveal an increase in oxidative stress after CPB, both in plasma and erythrocytes, and although the organism is capable of attenuating this stress by means of various antioxidative defence mechanisms, there is an increased possibility of post-CPB complications and thus of mortality. |
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