5-Hydroxytryptamine decreases somatostatin receptors and somatostatin-responsive adenylyl cyclase in rat pancreatic acinar membranes

Pretreatment of pancreatic acini with 5-hydroxytryptamine (5-HT) reduced the binding of the labeled somatostatin (SS) analogue 125I-Tyr3-SMS to pancreatic acinar membranes. This effect was dependent of the dose of 5-HT used and length of pretreatment. This inhibitory effect of 5-HT was abolished whe...

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Detalles Bibliográficos
Autores: Muñoz Acedo, Gema, Rodríguez Martín, Eulalia, Puebla Jiménez, Lilian|||0000-0002-0681-3235, Arilla Ferreiro, Eduardo
Tipo de recurso: artículo
Fecha de publicación:1997
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/2248
Acceso en línea:http://hdl.handle.net/10017/2248
https://dx.doi.org/10.1016/S0024-3205(97)00161-6
Access Level:acceso abierto
Palabra clave:Somatostatin
Serotonin
Pancreas
Bioquímica
Ciencia
Biochemistry
Science
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spelling 5-Hydroxytryptamine decreases somatostatin receptors and somatostatin-responsive adenylyl cyclase in rat pancreatic acinar membranesMuñoz Acedo, GemaRodríguez Martín, EulaliaPuebla Jiménez, Lilian|||0000-0002-0681-3235Arilla Ferreiro, EduardoSomatostatinSerotoninPancreasBioquímicaCienciaBiochemistrySciencePretreatment of pancreatic acini with 5-hydroxytryptamine (5-HT) reduced the binding of the labeled somatostatin (SS) analogue 125I-Tyr3-SMS to pancreatic acinar membranes. This effect was dependent of the dose of 5-HT used and length of pretreatment. This inhibitory effect of 5-HT was abolished when pancreatic acini were pretreated with 5-HT in the presence of the 5-HT1P receptor-antagonist 5-hydroxytryptophyl-5-hydroxytryptophan amide (5-HTP-DP). Pretreatment of pancreatic acini with 5-HT reduced the inhibition by the stable SS analogue SMS 201-995 of basal and forskolin (FK)-stimulated adenylyl cyclase (AC) activity in pancreatic acinar membranes. There was no statistical difference established between IC50 values for the stable GTP analogue 5'-guanylylimidodiphosphate (Gpp(NH)p) which inhibits ligand binding to SMS receptors in controls and in 5-HT treated pancreatic cells, respectively. In addition, no significant differences were seen in the level of Gi proteins in the control and 5-HT treated panceatic acini. These data suggest that the decrease of the number of 125I-Tyr3-SMS receptors, would explain the decreased sensitivity of AC to SMS 201-995 in membranes from 5-HT-pretreated acini.Universidad de AlcaláMinisterio de Educación y CulturaElsevier19971997-01-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/2248https://dx.doi.org/10.1016/S0024-3205(97)00161-6reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengUAH Not available 001%2F96 REGULACION DEL SISTEMA RECEPTOR-EFECTOR DE LA SOMATOSTATINA POR EL NEUROPEPTIDO Y (NPY) EN EL CEREBRO DE LA RATAMinisterio de Educación y Ciencia Not available PM95-0041 EFECTO DEL OXIDO NITRICO SOBRE EL SISTEMA RECEPTOR-EFECTOR DE LA SOMATOSTATINA EN EL CEREBRO HUMANO Y DE RATAopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/22482026-06-18T11:13:07Z
dc.title.none.fl_str_mv 5-Hydroxytryptamine decreases somatostatin receptors and somatostatin-responsive adenylyl cyclase in rat pancreatic acinar membranes
title 5-Hydroxytryptamine decreases somatostatin receptors and somatostatin-responsive adenylyl cyclase in rat pancreatic acinar membranes
spellingShingle 5-Hydroxytryptamine decreases somatostatin receptors and somatostatin-responsive adenylyl cyclase in rat pancreatic acinar membranes
Muñoz Acedo, Gema
Somatostatin
Serotonin
Pancreas
Bioquímica
Ciencia
Biochemistry
Science
title_short 5-Hydroxytryptamine decreases somatostatin receptors and somatostatin-responsive adenylyl cyclase in rat pancreatic acinar membranes
title_full 5-Hydroxytryptamine decreases somatostatin receptors and somatostatin-responsive adenylyl cyclase in rat pancreatic acinar membranes
title_fullStr 5-Hydroxytryptamine decreases somatostatin receptors and somatostatin-responsive adenylyl cyclase in rat pancreatic acinar membranes
title_full_unstemmed 5-Hydroxytryptamine decreases somatostatin receptors and somatostatin-responsive adenylyl cyclase in rat pancreatic acinar membranes
title_sort 5-Hydroxytryptamine decreases somatostatin receptors and somatostatin-responsive adenylyl cyclase in rat pancreatic acinar membranes
dc.creator.none.fl_str_mv Muñoz Acedo, Gema
Rodríguez Martín, Eulalia
Puebla Jiménez, Lilian|||0000-0002-0681-3235
Arilla Ferreiro, Eduardo
author Muñoz Acedo, Gema
author_facet Muñoz Acedo, Gema
Rodríguez Martín, Eulalia
Puebla Jiménez, Lilian|||0000-0002-0681-3235
Arilla Ferreiro, Eduardo
author_role author
author2 Rodríguez Martín, Eulalia
Puebla Jiménez, Lilian|||0000-0002-0681-3235
Arilla Ferreiro, Eduardo
author2_role author
author
author
dc.subject.none.fl_str_mv Somatostatin
Serotonin
Pancreas
Bioquímica
Ciencia
Biochemistry
Science
topic Somatostatin
Serotonin
Pancreas
Bioquímica
Ciencia
Biochemistry
Science
description Pretreatment of pancreatic acini with 5-hydroxytryptamine (5-HT) reduced the binding of the labeled somatostatin (SS) analogue 125I-Tyr3-SMS to pancreatic acinar membranes. This effect was dependent of the dose of 5-HT used and length of pretreatment. This inhibitory effect of 5-HT was abolished when pancreatic acini were pretreated with 5-HT in the presence of the 5-HT1P receptor-antagonist 5-hydroxytryptophyl-5-hydroxytryptophan amide (5-HTP-DP). Pretreatment of pancreatic acini with 5-HT reduced the inhibition by the stable SS analogue SMS 201-995 of basal and forskolin (FK)-stimulated adenylyl cyclase (AC) activity in pancreatic acinar membranes. There was no statistical difference established between IC50 values for the stable GTP analogue 5'-guanylylimidodiphosphate (Gpp(NH)p) which inhibits ligand binding to SMS receptors in controls and in 5-HT treated pancreatic cells, respectively. In addition, no significant differences were seen in the level of Gi proteins in the control and 5-HT treated panceatic acini. These data suggest that the decrease of the number of 125I-Tyr3-SMS receptors, would explain the decreased sensitivity of AC to SMS 201-995 in membranes from 5-HT-pretreated acini.
publishDate 1997
dc.date.none.fl_str_mv 1997
1997-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/2248
https://dx.doi.org/10.1016/S0024-3205(97)00161-6
url http://hdl.handle.net/10017/2248
https://dx.doi.org/10.1016/S0024-3205(97)00161-6
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv UAH Not available 001%2F96 REGULACION DEL SISTEMA RECEPTOR-EFECTOR DE LA SOMATOSTATINA POR EL NEUROPEPTIDO Y (NPY) EN EL CEREBRO DE LA RATA
Ministerio de Educación y Ciencia Not available PM95-0041 EFECTO DEL OXIDO NITRICO SOBRE EL SISTEMA RECEPTOR-EFECTOR DE LA SOMATOSTATINA EN EL CEREBRO HUMANO Y DE RATA
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
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