An engineered biomimetic peptide regulates cell behavior by synergistic integrin and growth factor signaling

Recreating the healing microenvironment is essential to regulate cell–material interactions and ensure the integration of biomaterials. To repair bone, such bioactivity can be achieved by mimicking its extracellular matrix (ECM) and by stimulating integrin and growth factor (GF) signaling. However,...

Descripción completa

Detalles Bibliográficos
Autores: Oliver Cervelló, Lluís|||0000-0001-5464-4758, Martín Gómez, Helena|||0000-0001-8956-116X, Reyes, Leslie, Noureddine, Fatima, Cavalcanti-Adam, Elisabetta Ada, Ginebra Molins, Maria Pau|||0000-0002-4700-5621, Mas Moruno, Carlos|||0000-0001-8337-0872
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/341486
Acceso en línea:https://hdl.handle.net/2117/341486
https://dx.doi.org/10.1002/adhm.202001757
Access Level:acceso abierto
Palabra clave:Biomimetics
Biomimètica
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
id ES_2e26f7fbc8a42a4d4b9c28b8fe973767
oai_identifier_str oai:upcommons.upc.edu:2117/341486
network_acronym_str ES
network_name_str España
repository_id_str
spelling An engineered biomimetic peptide regulates cell behavior by synergistic integrin and growth factor signalingOliver Cervelló, Lluís|||0000-0001-5464-4758Martín Gómez, Helena|||0000-0001-8956-116XReyes, LeslieNoureddine, FatimaCavalcanti-Adam, Elisabetta AdaGinebra Molins, Maria Pau|||0000-0002-4700-5621Mas Moruno, Carlos|||0000-0001-8337-0872BiomimeticsBiomimèticaÀrees temàtiques de la UPC::Enginyeria biomèdica::BiomaterialsRecreating the healing microenvironment is essential to regulate cell–material interactions and ensure the integration of biomaterials. To repair bone, such bioactivity can be achieved by mimicking its extracellular matrix (ECM) and by stimulating integrin and growth factor (GF) signaling. However, current approaches relying on the use of GFs, such as bone morphogenetic protein 2 (BMP-2), entail clinical risks. Here, a biomimetic peptide integrating the RGD cell adhesive sequence and the osteogenic DWIVA motif derived from the wrist epitope of BMP-2 is presented. The approach offers the advantage of having a spatial control over the single binding of integrins and BMP receptors. Such multifunctional platform is designed to incorporate 3,4-dihydroxyphenylalanine to bind metallic oxides with high affinity in a one step process. Functionalization of glass substrates with the engineered peptide is characterized by physicochemical methods, proving a successful surface modification. The biomimetic interfaces significantly improve the adhesion of C2C12 cells, inhibit myotube formation, and activate the BMP-dependent signaling via p38. These effects are not observed on surfaces displaying only one bioactive motif, a mixture of both motifs or soluble DWIVA. These data prove the biological potential of recreating the ECM and engaging in integrin and GF crosstalk via molecular-based mimics.Peer Reviewed20202020-12-1820212021-03-11journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/341486https://dx.doi.org/10.1002/adhm.202001757reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3414862026-05-27T15:37:01Z
dc.title.none.fl_str_mv An engineered biomimetic peptide regulates cell behavior by synergistic integrin and growth factor signaling
title An engineered biomimetic peptide regulates cell behavior by synergistic integrin and growth factor signaling
spellingShingle An engineered biomimetic peptide regulates cell behavior by synergistic integrin and growth factor signaling
Oliver Cervelló, Lluís|||0000-0001-5464-4758
Biomimetics
Biomimètica
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
title_short An engineered biomimetic peptide regulates cell behavior by synergistic integrin and growth factor signaling
title_full An engineered biomimetic peptide regulates cell behavior by synergistic integrin and growth factor signaling
title_fullStr An engineered biomimetic peptide regulates cell behavior by synergistic integrin and growth factor signaling
title_full_unstemmed An engineered biomimetic peptide regulates cell behavior by synergistic integrin and growth factor signaling
title_sort An engineered biomimetic peptide regulates cell behavior by synergistic integrin and growth factor signaling
dc.creator.none.fl_str_mv Oliver Cervelló, Lluís|||0000-0001-5464-4758
Martín Gómez, Helena|||0000-0001-8956-116X
Reyes, Leslie
Noureddine, Fatima
Cavalcanti-Adam, Elisabetta Ada
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
Mas Moruno, Carlos|||0000-0001-8337-0872
author Oliver Cervelló, Lluís|||0000-0001-5464-4758
author_facet Oliver Cervelló, Lluís|||0000-0001-5464-4758
Martín Gómez, Helena|||0000-0001-8956-116X
Reyes, Leslie
Noureddine, Fatima
Cavalcanti-Adam, Elisabetta Ada
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
Mas Moruno, Carlos|||0000-0001-8337-0872
author_role author
author2 Martín Gómez, Helena|||0000-0001-8956-116X
Reyes, Leslie
Noureddine, Fatima
Cavalcanti-Adam, Elisabetta Ada
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
Mas Moruno, Carlos|||0000-0001-8337-0872
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biomimetics
Biomimètica
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
topic Biomimetics
Biomimètica
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
description Recreating the healing microenvironment is essential to regulate cell–material interactions and ensure the integration of biomaterials. To repair bone, such bioactivity can be achieved by mimicking its extracellular matrix (ECM) and by stimulating integrin and growth factor (GF) signaling. However, current approaches relying on the use of GFs, such as bone morphogenetic protein 2 (BMP-2), entail clinical risks. Here, a biomimetic peptide integrating the RGD cell adhesive sequence and the osteogenic DWIVA motif derived from the wrist epitope of BMP-2 is presented. The approach offers the advantage of having a spatial control over the single binding of integrins and BMP receptors. Such multifunctional platform is designed to incorporate 3,4-dihydroxyphenylalanine to bind metallic oxides with high affinity in a one step process. Functionalization of glass substrates with the engineered peptide is characterized by physicochemical methods, proving a successful surface modification. The biomimetic interfaces significantly improve the adhesion of C2C12 cells, inhibit myotube formation, and activate the BMP-dependent signaling via p38. These effects are not observed on surfaces displaying only one bioactive motif, a mixture of both motifs or soluble DWIVA. These data prove the biological potential of recreating the ECM and engaging in integrin and GF crosstalk via molecular-based mimics.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-12-18
2021
2021-03-11
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/341486
https://dx.doi.org/10.1002/adhm.202001757
url https://hdl.handle.net/2117/341486
https://dx.doi.org/10.1002/adhm.202001757
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405384397029376
score 15,301603

Similares en LA Referencia