Identificación de especies de Dactylonectria e Ilyonectria asociadas al pie negro de la vid en Castilla la Mancha
[EN] Black foot is one of the most serious grapevine trunk diseases worldwide. This disease is caused by soilborne fungal species included into several genera and it is one of the most common diseases in young vines in Spain, especially in Castilla La Mancha region. Since 2002, the cases of young vi...
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| Tipo de recurso: | tesis de maestría |
| Fecha de publicación: | 2015 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | español |
| OAI Identifier: | oai:riunet.upv.es:10251/66813 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/66813 |
| Access Level: | acceso abierto |
| Palabra clave: | Pie negro Ilyonectria Dactylonectria Análisis filogenético PCR Vitis vinifera Black foot Phylogenetic analysis PRODUCCION VEGETAL Máster Universitario en Sanidad y Producción Vegetal-Màster Universitari en Sanitat i Producció Vegetal |
| Sumario: | [EN] Black foot is one of the most serious grapevine trunk diseases worldwide. This disease is caused by soilborne fungal species included into several genera and it is one of the most common diseases in young vines in Spain, especially in Castilla La Mancha region. Since 2002, the cases of young vines showing decline symptoms, analysed by the Servicio de Diagnóstico y Asistencia Fitosanitaria (SEDAF) from the Instituto Técnico Agronómico Provincial de Albacete (ITAP) have increased, being the fungi associated with black foot disease the most frequently detected. Fungal identification on this laboratory is currently based only on morphological data, however this methodology shows several disadvantages that hinder the correct species identification. The main aim of the present study was to set up a molecular method for the accurate identification of black foot pathogens in the SEDAF laboratory. This method is based on monosporic cultures establishment, DNA extraction, DNA amplification by PCR using a set of primers for histone H3 gene, sequencing of PCR products and phylogenetic analysis. Results showed that this protocol was set up properly and it was used for the identification of 26 isolates obtained from grapevines showing symptoms of black foot disease during the years 2014 and 2015. All isolates were identified as belonging to five different species: Dactylonectria torresensis, D. alcacerensis, D. novozelandica, D. macrodidyma e Ilyonectria liriodendri |
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