The Greatwall-Endosulfine Switch Accelerates Autophagic Flux during the Cell Divisions Leading to G1 Arrest and Entry into Quiescence in Fission Yeast

Entry into quiescence in the fission yeast Schizosaccharomyces pombe is induced by nitrogen starvation. In the absence of nitrogen, proliferating fission yeast cells divide twice without cell growth and undergo cell cycle arrest in G1 before becoming G0 quiescent cells. Under these conditions, autop...

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Autores: Vázquez-Bolado, Alicia, López-San Segundo, Rafael, García-Blanco, Natalia, Rozalén, Ana E., González-Álvarez, Daniel, Suárez, M. Belén, Pérez-Hidalgo, Livia, Moreno, Sergio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::78df0ec014b97b0b6cd2ac7b0bd918df
Acceso en línea:http://hdl.handle.net/10261/336882
Access Level:acceso abierto
Palabra clave:Autophagy
Quiescence
Greatwall
Endosulfine
PP2A
Fission yeast
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spelling The Greatwall-Endosulfine Switch Accelerates Autophagic Flux during the Cell Divisions Leading to G1 Arrest and Entry into Quiescence in Fission YeastVázquez-Bolado, AliciaLópez-San Segundo, RafaelGarcía-Blanco, NataliaRozalén, Ana E.González-Álvarez, DanielSuárez, M. BelénPérez-Hidalgo, LiviaMoreno, SergioAutophagyQuiescenceGreatwallEndosulfinePP2AFission yeastEntry into quiescence in the fission yeast Schizosaccharomyces pombe is induced by nitrogen starvation. In the absence of nitrogen, proliferating fission yeast cells divide twice without cell growth and undergo cell cycle arrest in G1 before becoming G0 quiescent cells. Under these conditions, autophagy is induced to produce enough nitrogen for the two successive cell divisions that take place before the G1 arrest. In parallel to the induction of autophagy, the Greatwall–Endosulfine switch is activated upon nitrogen starvation to down-regulate protein phosphatase PP2A/B55 activity, which is essential for cell cycle arrest in G1 and implementation of the quiescent program. Here we show that, although inactivation of PP2A/B55 by the Greatwall–Endosulfine switch is not required to promote autophagy initiation, it increases autophagic flux at least in part by upregulating the expression of a number of autophagy-related genes.This work was funded by the Spanish Ministry of Science and Innovation-MICINN (grants BFU2017-88335-R and PID2020-117218RB-I00) and from the Castile and Leon government (grants CSI151U13 and CSI259P20 and IBFG Unit of Excellence programs CLU-2017-03 and CL-EI-2021-08 co-funded by the P.O. Feder of Castile and Leon 14-20 and European Union ERDF “Europe drives our growth”). N.G.-B., A.V.B. and D.G.-Á. were funded by FPU15/03654, BES-2015-073171, PRE2018-084956 predoctoral training contracts. R.L.-S.S. was funded by a predoctoral fellowship from the Castile and Leon governments.Peer reviewedMolecular Diversity Preservation InternationalMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Junta de Castilla y LeónEuropean CommissionMinisterio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2023202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/336882reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2017-88335-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-117218RB-I00info:eu-repo/grantAgreement/MECD//FPU15%2F03654info:eu-repo/grantAgreement/MINECO//BES-2015-073171info:eu-repo/grantAgreement/AEI//PRE2018-084956The underlying dataset has been published as supplementary material of the article in the publisher platform at http://dx.doi.org/10.3390/ijms24010148http://dx.doi.org/10.3390/ijms24010148Síinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::78df0ec014b97b0b6cd2ac7b0bd918df2026-05-22T06:33:51Z
dc.title.none.fl_str_mv The Greatwall-Endosulfine Switch Accelerates Autophagic Flux during the Cell Divisions Leading to G1 Arrest and Entry into Quiescence in Fission Yeast
title The Greatwall-Endosulfine Switch Accelerates Autophagic Flux during the Cell Divisions Leading to G1 Arrest and Entry into Quiescence in Fission Yeast
spellingShingle The Greatwall-Endosulfine Switch Accelerates Autophagic Flux during the Cell Divisions Leading to G1 Arrest and Entry into Quiescence in Fission Yeast
Vázquez-Bolado, Alicia
Autophagy
Quiescence
Greatwall
Endosulfine
PP2A
Fission yeast
title_short The Greatwall-Endosulfine Switch Accelerates Autophagic Flux during the Cell Divisions Leading to G1 Arrest and Entry into Quiescence in Fission Yeast
title_full The Greatwall-Endosulfine Switch Accelerates Autophagic Flux during the Cell Divisions Leading to G1 Arrest and Entry into Quiescence in Fission Yeast
title_fullStr The Greatwall-Endosulfine Switch Accelerates Autophagic Flux during the Cell Divisions Leading to G1 Arrest and Entry into Quiescence in Fission Yeast
title_full_unstemmed The Greatwall-Endosulfine Switch Accelerates Autophagic Flux during the Cell Divisions Leading to G1 Arrest and Entry into Quiescence in Fission Yeast
title_sort The Greatwall-Endosulfine Switch Accelerates Autophagic Flux during the Cell Divisions Leading to G1 Arrest and Entry into Quiescence in Fission Yeast
dc.creator.none.fl_str_mv Vázquez-Bolado, Alicia
López-San Segundo, Rafael
García-Blanco, Natalia
Rozalén, Ana E.
González-Álvarez, Daniel
Suárez, M. Belén
Pérez-Hidalgo, Livia
Moreno, Sergio
author Vázquez-Bolado, Alicia
author_facet Vázquez-Bolado, Alicia
López-San Segundo, Rafael
García-Blanco, Natalia
Rozalén, Ana E.
González-Álvarez, Daniel
Suárez, M. Belén
Pérez-Hidalgo, Livia
Moreno, Sergio
author_role author
author2 López-San Segundo, Rafael
García-Blanco, Natalia
Rozalén, Ana E.
González-Álvarez, Daniel
Suárez, M. Belén
Pérez-Hidalgo, Livia
Moreno, Sergio
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
Junta de Castilla y León
European Commission
Ministerio de Ciencia, Innovación y Universidades (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Autophagy
Quiescence
Greatwall
Endosulfine
PP2A
Fission yeast
topic Autophagy
Quiescence
Greatwall
Endosulfine
PP2A
Fission yeast
description Entry into quiescence in the fission yeast Schizosaccharomyces pombe is induced by nitrogen starvation. In the absence of nitrogen, proliferating fission yeast cells divide twice without cell growth and undergo cell cycle arrest in G1 before becoming G0 quiescent cells. Under these conditions, autophagy is induced to produce enough nitrogen for the two successive cell divisions that take place before the G1 arrest. In parallel to the induction of autophagy, the Greatwall–Endosulfine switch is activated upon nitrogen starvation to down-regulate protein phosphatase PP2A/B55 activity, which is essential for cell cycle arrest in G1 and implementation of the quiescent program. Here we show that, although inactivation of PP2A/B55 by the Greatwall–Endosulfine switch is not required to promote autophagy initiation, it increases autophagic flux at least in part by upregulating the expression of a number of autophagy-related genes.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/336882
url http://hdl.handle.net/10261/336882
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2017-88335-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-117218RB-I00
info:eu-repo/grantAgreement/MECD//FPU15%2F03654
info:eu-repo/grantAgreement/MINECO//BES-2015-073171
info:eu-repo/grantAgreement/AEI//PRE2018-084956
The underlying dataset has been published as supplementary material of the article in the publisher platform at http://dx.doi.org/10.3390/ijms24010148
http://dx.doi.org/10.3390/ijms24010148
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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