Characterization of a Bacillus subtilis 64-kDa DNA polymerase X potentially involved in DNA repair
Bacillus subtilis gene yshC encodes a 64 kDa family X DNA polymerase (PolXBs), which contains all the critical residues involved in DNA and nucleotide binding, as well as those responsible for catalysis of DNA polymerization, conserved in most family X members. Biochemical analyses of the purified e...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2008 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:dnet:digitalcsic_::19dd066c09a7ebfe26ad2dcb053924ef |
| Acceso en línea: | http://hdl.handle.net/10261/34089 |
| Access Level: | acceso abierto |
| Palabra clave: | DNA polymerase Family X/ Bacillus subtilis Polβ DNA repair |
| Sumario: | Bacillus subtilis gene yshC encodes a 64 kDa family X DNA polymerase (PolXBs), which contains all the critical residues involved in DNA and nucleotide binding, as well as those responsible for catalysis of DNA polymerization, conserved in most family X members. Biochemical analyses of the purified enzyme indicate that PolXBs is a monomeric and strictly template-directed DNA polymerase, preferentially acting on DNA structures containing gaps from one to few nucleotides and bearing a phosphate group at the 5´ end of the downstream DNA. The fact that PolXBs is able to conduct filling of a single nucleotide gap, allowing further sealing of the resulting nick by a DNA ligase points to a putative role in base excision repair during the B. subtilis life cycle. |
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