Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie

Scrapie is a transmissible spongiform encephalopathy with a wide PrPres dissemination in many non-neural tissues and with high levels of transmissibility within susceptible populations. Mechanisms of transmission are incompletely understood. It is generally assumed that it is horizontally transmitte...

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Autores: Garza, M.C., Fernández-Borges, N., Bolea, R., Badiola, J.J., Castilla, J., Monleón, E.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:España
Institución:Universidad de Zaragoza
Repositorio:Zaguán. Repositorio Digital de la Universidad de Zaragoza
OAI Identifier:oai:zaguan.unizar.es:61331
Acceso en línea:http://zaguan.unizar.es/record/61331
Access Level:acceso abierto
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spelling Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapieGarza, M.C.Fernández-Borges, N.Bolea, R.Badiola, J.J.Castilla, J.Monleón, E.Scrapie is a transmissible spongiform encephalopathy with a wide PrPres dissemination in many non-neural tissues and with high levels of transmissibility within susceptible populations. Mechanisms of transmission are incompletely understood. It is generally assumed that it is horizontally transmitted by direct contact between animals or indirectly through the environment, where scrapie can remain infectious for years. In contrast, in utero vertical transmission has never been demonstrated and has rarely been studied. Recently, the use of the protein misfolding cyclic amplification technique (PMCA) has allowed prion detection in various tissues and excretions in which PrPres levels have been undetectable by traditional assays. The main goal of this study was to detect PrPres in fetal tissues and the amniotic fluid from natural scrapie infected ewes using the PMCA technique. Six fetuses from three infected pregnant ewes in an advanced clinical stage of the disease were included in the study. From each fetus, amniotic fluid, brain, spleen, ileo-cecal valve and retropharyngeal lymph node samples were collected and analyzed using Western blotting and PMCA. Although all samples were negative using Western blotting, PrPres was detected after in vitro amplification. Our results represent the first time the biochemical detection of prions in fetal tissues, suggesting that the in utero transmission of scrapie in natural infected sheep might be possible.2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://zaguan.unizar.es/record/61331reponame:Zaguán. Repositorio Digital de la Universidad de Zaragozainstname:Universidad de ZaragozaInglésinfo:eu-repo/grantAgreement/ES/MEC/AP2007-03842info:eu-repo/grantAgreement/ES/MICINN/AGL2009-11553-C02-01info:eu-repo/semantics/openAccessoai:zaguan.unizar.es:613312026-05-29T13:59:51Z
dc.title.none.fl_str_mv Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
title Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
spellingShingle Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
Garza, M.C.
title_short Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
title_full Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
title_fullStr Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
title_full_unstemmed Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
title_sort Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
dc.creator.none.fl_str_mv Garza, M.C.
Fernández-Borges, N.
Bolea, R.
Badiola, J.J.
Castilla, J.
Monleón, E.
author Garza, M.C.
author_facet Garza, M.C.
Fernández-Borges, N.
Bolea, R.
Badiola, J.J.
Castilla, J.
Monleón, E.
author_role author
author2 Fernández-Borges, N.
Bolea, R.
Badiola, J.J.
Castilla, J.
Monleón, E.
author2_role author
author
author
author
author
description Scrapie is a transmissible spongiform encephalopathy with a wide PrPres dissemination in many non-neural tissues and with high levels of transmissibility within susceptible populations. Mechanisms of transmission are incompletely understood. It is generally assumed that it is horizontally transmitted by direct contact between animals or indirectly through the environment, where scrapie can remain infectious for years. In contrast, in utero vertical transmission has never been demonstrated and has rarely been studied. Recently, the use of the protein misfolding cyclic amplification technique (PMCA) has allowed prion detection in various tissues and excretions in which PrPres levels have been undetectable by traditional assays. The main goal of this study was to detect PrPres in fetal tissues and the amniotic fluid from natural scrapie infected ewes using the PMCA technique. Six fetuses from three infected pregnant ewes in an advanced clinical stage of the disease were included in the study. From each fetus, amniotic fluid, brain, spleen, ileo-cecal valve and retropharyngeal lymph node samples were collected and analyzed using Western blotting and PMCA. Although all samples were negative using Western blotting, PrPres was detected after in vitro amplification. Our results represent the first time the biochemical detection of prions in fetal tissues, suggesting that the in utero transmission of scrapie in natural infected sheep might be possible.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.none.fl_str_mv http://zaguan.unizar.es/record/61331
url http://zaguan.unizar.es/record/61331
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/ES/MEC/AP2007-03842
info:eu-repo/grantAgreement/ES/MICINN/AGL2009-11553-C02-01
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dc.source.none.fl_str_mv reponame:Zaguán. Repositorio Digital de la Universidad de Zaragoza
instname:Universidad de Zaragoza
instname_str Universidad de Zaragoza
reponame_str Zaguán. Repositorio Digital de la Universidad de Zaragoza
collection Zaguán. Repositorio Digital de la Universidad de Zaragoza
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