Skin multi-omics-based interactome analysis: integrating the tissue and mucus exuded layer for a comprehensive understanding of the teleost mucosa functionality as model of study
From a general structural perspective, a mucosal tissue is constituted by two main matrices: the tissue and the secreted mucus. Jointly, they fulfill a wide range of functions including the protection of the epithelial layer. In this study, we simultaneously analyzed the epithelial tissue and the se...
| Autores: | , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/175768 |
| Acceso en línea: | https://hdl.handle.net/2445/175768 |
| Access Level: | acceso abierto |
| Palabra clave: | Membrana mucosa Peixos Mucous membrane Fishes |
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Skin multi-omics-based interactome analysis: integrating the tissue and mucus exuded layer for a comprehensive understanding of the teleost mucosa functionality as model of studyReyes López, Felipe E.Ibarz i Valls, AntoniOrdóñez-Grande, BorjaVallejos Vidal, EvaAndree, Karl B.Balasch, Joan CarlesFernández-Alacid, LauraSanahuja Piera, IgnasiSánchez Nuño, SergioFirmino, Joana P.Pavez, LeonardoPolo Pozo, Francisco JavierTort Bardolet, LluísGisbert Casas, EnricMembrana mucosaPeixosMucous membraneFishesFrom a general structural perspective, a mucosal tissue is constituted by two main matrices: the tissue and the secreted mucus. Jointly, they fulfill a wide range of functions including the protection of the epithelial layer. In this study, we simultaneously analyzed the epithelial tissue and the secreted mucus response using a holistic interactome-based multi-omics approach. The effect of the gilthead sea bream (Sparus aurata) skin mucosa to a dietary inclusion of spray-dried porcine plasma (SDPP) was evaluated. The epithelial skin microarrays-based transcriptome data showed 194 differentially expressed genes, meanwhile the exuded mucus proteome analysis 35 differentially synthesized proteins. Separately, the skin transcripteractome revealed an expression profile that favored biological mechanisms associated to gene expression, biogenesis, vesicle function, protein transport and localization to the membrane. Mucus proteome showed an enhanced protective role with putatively higher antioxidant and antimicrobial properties. The integrated skin mucosa multi-interactome analysis evidenced the interrelationship and synergy between the metabolism and the exuded mucus functions improving specifically the tissue development, innate defenses, and environment recognition. Histologically, the skin increased in thickness and in number of mucous cells. A positive impact on animal performance, growth and feed efficiency was also registered. Collectively, the results suggest an intimate crosstalk between skin tissue and its exuded mucus in response to the nutritional stimulus (SDPP supplementation) that favors the stimulation of cell protein turnover and the activation of the exudation machinery in the skin mucosa. Thus, the multi-omics-based interactome analysis provides a comprehensive understanding of the biological context of response that takes place in a mucosal tissue. In perspective, this strategy is applicable for evaluating the effect of any experimental variable on any mucosal tissue functionality, including the benefits this assessment may provide on the study of the mammalian mucosa.Frontiers Media2021202120212021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion20 p.application/pdfhttps://hdl.handle.net/2445/175768Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3389/fimmu.2020.613824Frontiers in Immunology, 2021, vol. 11, num. 613824https://doi.org/10.3389/fimmu.2020.613824info:eu-repo/grantAgreement/EC/H2020/727315cc-by (c) Reyes-López, F.R. et al., 2021http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1757682026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Skin multi-omics-based interactome analysis: integrating the tissue and mucus exuded layer for a comprehensive understanding of the teleost mucosa functionality as model of study |
| title |
Skin multi-omics-based interactome analysis: integrating the tissue and mucus exuded layer for a comprehensive understanding of the teleost mucosa functionality as model of study |
| spellingShingle |
Skin multi-omics-based interactome analysis: integrating the tissue and mucus exuded layer for a comprehensive understanding of the teleost mucosa functionality as model of study Reyes López, Felipe E. Membrana mucosa Peixos Mucous membrane Fishes |
| title_short |
Skin multi-omics-based interactome analysis: integrating the tissue and mucus exuded layer for a comprehensive understanding of the teleost mucosa functionality as model of study |
| title_full |
Skin multi-omics-based interactome analysis: integrating the tissue and mucus exuded layer for a comprehensive understanding of the teleost mucosa functionality as model of study |
| title_fullStr |
Skin multi-omics-based interactome analysis: integrating the tissue and mucus exuded layer for a comprehensive understanding of the teleost mucosa functionality as model of study |
| title_full_unstemmed |
Skin multi-omics-based interactome analysis: integrating the tissue and mucus exuded layer for a comprehensive understanding of the teleost mucosa functionality as model of study |
| title_sort |
Skin multi-omics-based interactome analysis: integrating the tissue and mucus exuded layer for a comprehensive understanding of the teleost mucosa functionality as model of study |
| dc.creator.none.fl_str_mv |
Reyes López, Felipe E. Ibarz i Valls, Antoni Ordóñez-Grande, Borja Vallejos Vidal, Eva Andree, Karl B. Balasch, Joan Carles Fernández-Alacid, Laura Sanahuja Piera, Ignasi Sánchez Nuño, Sergio Firmino, Joana P. Pavez, Leonardo Polo Pozo, Francisco Javier Tort Bardolet, Lluís Gisbert Casas, Enric |
| author |
Reyes López, Felipe E. |
| author_facet |
Reyes López, Felipe E. Ibarz i Valls, Antoni Ordóñez-Grande, Borja Vallejos Vidal, Eva Andree, Karl B. Balasch, Joan Carles Fernández-Alacid, Laura Sanahuja Piera, Ignasi Sánchez Nuño, Sergio Firmino, Joana P. Pavez, Leonardo Polo Pozo, Francisco Javier Tort Bardolet, Lluís Gisbert Casas, Enric |
| author_role |
author |
| author2 |
Ibarz i Valls, Antoni Ordóñez-Grande, Borja Vallejos Vidal, Eva Andree, Karl B. Balasch, Joan Carles Fernández-Alacid, Laura Sanahuja Piera, Ignasi Sánchez Nuño, Sergio Firmino, Joana P. Pavez, Leonardo Polo Pozo, Francisco Javier Tort Bardolet, Lluís Gisbert Casas, Enric |
| author2_role |
author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Membrana mucosa Peixos Mucous membrane Fishes |
| topic |
Membrana mucosa Peixos Mucous membrane Fishes |
| description |
From a general structural perspective, a mucosal tissue is constituted by two main matrices: the tissue and the secreted mucus. Jointly, they fulfill a wide range of functions including the protection of the epithelial layer. In this study, we simultaneously analyzed the epithelial tissue and the secreted mucus response using a holistic interactome-based multi-omics approach. The effect of the gilthead sea bream (Sparus aurata) skin mucosa to a dietary inclusion of spray-dried porcine plasma (SDPP) was evaluated. The epithelial skin microarrays-based transcriptome data showed 194 differentially expressed genes, meanwhile the exuded mucus proteome analysis 35 differentially synthesized proteins. Separately, the skin transcripteractome revealed an expression profile that favored biological mechanisms associated to gene expression, biogenesis, vesicle function, protein transport and localization to the membrane. Mucus proteome showed an enhanced protective role with putatively higher antioxidant and antimicrobial properties. The integrated skin mucosa multi-interactome analysis evidenced the interrelationship and synergy between the metabolism and the exuded mucus functions improving specifically the tissue development, innate defenses, and environment recognition. Histologically, the skin increased in thickness and in number of mucous cells. A positive impact on animal performance, growth and feed efficiency was also registered. Collectively, the results suggest an intimate crosstalk between skin tissue and its exuded mucus in response to the nutritional stimulus (SDPP supplementation) that favors the stimulation of cell protein turnover and the activation of the exudation machinery in the skin mucosa. Thus, the multi-omics-based interactome analysis provides a comprehensive understanding of the biological context of response that takes place in a mucosal tissue. In perspective, this strategy is applicable for evaluating the effect of any experimental variable on any mucosal tissue functionality, including the benefits this assessment may provide on the study of the mammalian mucosa. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2021 2021 2021 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/175768 |
| url |
https://hdl.handle.net/2445/175768 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: https://doi.org/10.3389/fimmu.2020.613824 Frontiers in Immunology, 2021, vol. 11, num. 613824 https://doi.org/10.3389/fimmu.2020.613824 info:eu-repo/grantAgreement/EC/H2020/727315 |
| dc.rights.none.fl_str_mv |
cc-by (c) Reyes-López, F.R. et al., 2021 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
cc-by (c) Reyes-López, F.R. et al., 2021 http://creativecommons.org/licenses/by/3.0/es |
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openAccess |
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20 p. application/pdf |
| dc.publisher.none.fl_str_mv |
Frontiers Media |
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Frontiers Media |
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Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Recercat. Dipósit de la Recerca de Catalunya |
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