Neospora caninum infection as a cause of reproductive failure in a sheep flock
[EN] Neospora caninum has been detected only sporadically in cases of ovine abortion, and it has therefore traditionally been considered as an unimportant parasite in small ruminants. This study was carried out with the aim of identifying the pathogen causing serious reproductive problems on a comme...
| Autores: | , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | España |
| Institución: | Universidad de León |
| Repositorio: | BULERIA. Repositorio Institucional de la Universidad de León |
| OAI Identifier: | oai:buleria.unileon.es:10612/25726 |
| Acceso en línea: | https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-014-0088-5 https://hdl.handle.net/10612/25726 |
| Access Level: | acceso abierto |
| Palabra clave: | Sanidad animal Veterinaria Neospora caninum Toxoplasma gondii Reproductive failure Sheep PCR 2401.12 Parasitología Animal |
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Neospora caninum infection as a cause of reproductive failure in a sheep flockGonzález Warleta, MartaCastro Hermida, José AntonioRegidor Cerrillo, JavierBenavides Silván, JulioÁlvarez García, GemaFuertes Franco, MiguelOrtega Mora, Luis MiguelMezo, MercedesSanidad animalVeterinariaNeospora caninumToxoplasma gondiiReproductive failureSheepPCR2401.12 Parasitología Animal[EN] Neospora caninum has been detected only sporadically in cases of ovine abortion, and it has therefore traditionally been considered as an unimportant parasite in small ruminants. This study was carried out with the aim of identifying the pathogen causing serious reproductive problems on a commercial sheep farm. Sera from all rams and ewes tested negative for antibodies against Border disease virus, Schmallenberg virus and Coxiella burnetii, and infections by these agents were therefore ruled out. Nevertheless, seropositivity to N. caninum and/or Toxoplasma gondii was detected, although the seroprevalence was higher in the case of N. caninum. The percentage of lambings and the number of lambs per dam were significantly lower in ewes that were seropositive to N. caninum while no effect on these parameters was detected in ewes that were seropositive to T. gondii. There was also no evidence of infection by T. gondii in the foetal/lamb tissues analyzed by PCR and/or immunohistopathological techniques. On the contrary, the DNA of N. caninum was detected in 13 out of 14 foetuses/lambs descendant from dams seropositive to this parasite. Characteristic lesions caused by N. caninum and/or its antigen were also detected. Genotyping of the N. caninum DNA revealed only two closely related microsatellite multilocus genotypes. The results clearly demonstrate that infection by N. caninum was the cause of the low reproductive performance of this sheep flockSIThe authors gratefully acknowledge Carmen Carro and Xabier Cortizo from CIAM-INGACAL for their excellent technical assistance. Special thanks are due to the farm owner for his helpful collaboration. The present study was supported by Xunta de Galicia (FEADER 2013/15)BioMed CentralSanidad AnimalFacultad de Veterinaria2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-014-0088-5https://hdl.handle.net/10612/25726reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónIngléshttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/257262026-06-24T12:43:27Z |
| dc.title.none.fl_str_mv |
Neospora caninum infection as a cause of reproductive failure in a sheep flock |
| title |
Neospora caninum infection as a cause of reproductive failure in a sheep flock |
| spellingShingle |
Neospora caninum infection as a cause of reproductive failure in a sheep flock González Warleta, Marta Sanidad animal Veterinaria Neospora caninum Toxoplasma gondii Reproductive failure Sheep PCR 2401.12 Parasitología Animal |
| title_short |
Neospora caninum infection as a cause of reproductive failure in a sheep flock |
| title_full |
Neospora caninum infection as a cause of reproductive failure in a sheep flock |
| title_fullStr |
Neospora caninum infection as a cause of reproductive failure in a sheep flock |
| title_full_unstemmed |
Neospora caninum infection as a cause of reproductive failure in a sheep flock |
| title_sort |
Neospora caninum infection as a cause of reproductive failure in a sheep flock |
| dc.creator.none.fl_str_mv |
González Warleta, Marta Castro Hermida, José Antonio Regidor Cerrillo, Javier Benavides Silván, Julio Álvarez García, Gema Fuertes Franco, Miguel Ortega Mora, Luis Miguel Mezo, Mercedes |
| author |
González Warleta, Marta |
| author_facet |
González Warleta, Marta Castro Hermida, José Antonio Regidor Cerrillo, Javier Benavides Silván, Julio Álvarez García, Gema Fuertes Franco, Miguel Ortega Mora, Luis Miguel Mezo, Mercedes |
| author_role |
author |
| author2 |
Castro Hermida, José Antonio Regidor Cerrillo, Javier Benavides Silván, Julio Álvarez García, Gema Fuertes Franco, Miguel Ortega Mora, Luis Miguel Mezo, Mercedes |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Sanidad Animal Facultad de Veterinaria |
| dc.subject.none.fl_str_mv |
Sanidad animal Veterinaria Neospora caninum Toxoplasma gondii Reproductive failure Sheep PCR 2401.12 Parasitología Animal |
| topic |
Sanidad animal Veterinaria Neospora caninum Toxoplasma gondii Reproductive failure Sheep PCR 2401.12 Parasitología Animal |
| description |
[EN] Neospora caninum has been detected only sporadically in cases of ovine abortion, and it has therefore traditionally been considered as an unimportant parasite in small ruminants. This study was carried out with the aim of identifying the pathogen causing serious reproductive problems on a commercial sheep farm. Sera from all rams and ewes tested negative for antibodies against Border disease virus, Schmallenberg virus and Coxiella burnetii, and infections by these agents were therefore ruled out. Nevertheless, seropositivity to N. caninum and/or Toxoplasma gondii was detected, although the seroprevalence was higher in the case of N. caninum. The percentage of lambings and the number of lambs per dam were significantly lower in ewes that were seropositive to N. caninum while no effect on these parameters was detected in ewes that were seropositive to T. gondii. There was also no evidence of infection by T. gondii in the foetal/lamb tissues analyzed by PCR and/or immunohistopathological techniques. On the contrary, the DNA of N. caninum was detected in 13 out of 14 foetuses/lambs descendant from dams seropositive to this parasite. Characteristic lesions caused by N. caninum and/or its antigen were also detected. Genotyping of the N. caninum DNA revealed only two closely related microsatellite multilocus genotypes. The results clearly demonstrate that infection by N. caninum was the cause of the low reproductive performance of this sheep flock |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-014-0088-5 https://hdl.handle.net/10612/25726 |
| url |
https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-014-0088-5 https://hdl.handle.net/10612/25726 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.rights.none.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
BioMed Central |
| publisher.none.fl_str_mv |
BioMed Central |
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reponame:BULERIA. Repositorio Institucional de la Universidad de León instname:Universidad de León |
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Universidad de León |
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BULERIA. Repositorio Institucional de la Universidad de León |
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BULERIA. Repositorio Institucional de la Universidad de León |
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15.81155 |