Efficacy of the CHRONOlight biodynamic lighting system for control of nosocomial Gram-negative pathogens

Objectives Health care–associated infections are a growing public health concern, with hospitals acting as reservoirs for pathogens. The rise in antimicrobial resistance worsens the issue. This study tested the effectiveness of the biodynamic lighting system (CHRONOlight) at varying ultraviolet (UV)...

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Autores: Pérez Palacios, Patricia, Bustamante, Pedro, Domínguez Amarillo, Samuel, Caro Ayora, María José, Acosta García, Ignacio Javier, Rodríguez Martínez, José Manuel
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/178506
Acesso em linha:https://hdl.handle.net/11441/178506
https://doi.org/10.1016/j.ijid.2025.108120
Access Level:acceso abierto
Palavra-chave:UV-C light disinfection
Gram-negative pathogens
Healthcare-associated infections
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spelling Efficacy of the CHRONOlight biodynamic lighting system for control of nosocomial Gram-negative pathogensPérez Palacios, PatriciaBustamante, PedroDomínguez Amarillo, SamuelCaro Ayora, María JoséAcosta García, Ignacio JavierRodríguez Martínez, José ManuelUV-C light disinfectionGram-negative pathogensHealthcare-associated infectionsObjectives Health care–associated infections are a growing public health concern, with hospitals acting as reservoirs for pathogens. The rise in antimicrobial resistance worsens the issue. This study tested the effectiveness of the biodynamic lighting system (CHRONOlight) at varying ultraviolet (UV) light times and energy levels to reduce nosocomial gram-negative pathogens. Methods Eight clinical isolates, representing different gram-negative pathogens, were selected from the Andalusian reference laboratory. Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii ATCC were used as control strains. Different inoculums were exposed to type C UV light (UV-C) doses 2.0, 1.5, and 1.0 W/m2 (corresponding to an irradiation flux of 100%, 75%, and 50%, respectively, with spectral peaks at 270 nm) during 15, 30, 45, 60, and 300 seconds and type A UV light (UV-A) (380 nm) during 30 minutes. The bactericidal effect was considered when cellular viability decreased by ≥3-log10 colony forming unit/ml. UV-C light-surviving isolates were sequenced (Illumina MiSeq) and analyzed to identify mutations associated with UV-C light survival. The development of antibiotic resistance after UV-C exposure were also studied for the same surviving isolates. Results The efficacy of the CHRONOlight biodynamic lighting system varied by exposure time, UV dose, and bacterial species. E. coli isolates showed bactericidal effects within 15-30 seconds at 100% UV-C intensity, whereas Klebsiella pneumoniae required 30-45 seconds. P. aeruginosa and A. baumannii showed activity in 15-30 seconds, but clinical A. baumannii needed longer exposure, especially at lower UV-C intensities. No reduction in cell viability was seen with UV-A exposure. Gene mutations related to metabolism, stress response, motility, and virulence were found in survivors, and no cross-antibiotic resistance developed in surviving isolates. Conclusions CHRONOlight biodynamic lighting system is a novel broad-spectrum lighting prototype designed to effectively reduce the viability of gram-negative bacteria within short exposure times (30-300 seconds) through UV-C emission. This makes it a promising strategy for minimizing nosocomial infections in health care settings.ElsevierConstrucciones Arquitectónicas IMicrobiología2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/178506https://doi.org/10.1016/j.ijid.2025.108120reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésInternational Journal of Infectious Diseases, 161, 108120.PID2020-117563RB-I00https://www.ijidonline.com/article/S1201-9712(25)00342-Xinfo:eu-repo/semantics/openAccessoai:idus.us.es:11441/1785062026-06-17T12:51:07Z
dc.title.none.fl_str_mv Efficacy of the CHRONOlight biodynamic lighting system for control of nosocomial Gram-negative pathogens
title Efficacy of the CHRONOlight biodynamic lighting system for control of nosocomial Gram-negative pathogens
spellingShingle Efficacy of the CHRONOlight biodynamic lighting system for control of nosocomial Gram-negative pathogens
Pérez Palacios, Patricia
UV-C light disinfection
Gram-negative pathogens
Healthcare-associated infections
title_short Efficacy of the CHRONOlight biodynamic lighting system for control of nosocomial Gram-negative pathogens
title_full Efficacy of the CHRONOlight biodynamic lighting system for control of nosocomial Gram-negative pathogens
title_fullStr Efficacy of the CHRONOlight biodynamic lighting system for control of nosocomial Gram-negative pathogens
title_full_unstemmed Efficacy of the CHRONOlight biodynamic lighting system for control of nosocomial Gram-negative pathogens
title_sort Efficacy of the CHRONOlight biodynamic lighting system for control of nosocomial Gram-negative pathogens
dc.creator.none.fl_str_mv Pérez Palacios, Patricia
Bustamante, Pedro
Domínguez Amarillo, Samuel
Caro Ayora, María José
Acosta García, Ignacio Javier
Rodríguez Martínez, José Manuel
author Pérez Palacios, Patricia
author_facet Pérez Palacios, Patricia
Bustamante, Pedro
Domínguez Amarillo, Samuel
Caro Ayora, María José
Acosta García, Ignacio Javier
Rodríguez Martínez, José Manuel
author_role author
author2 Bustamante, Pedro
Domínguez Amarillo, Samuel
Caro Ayora, María José
Acosta García, Ignacio Javier
Rodríguez Martínez, José Manuel
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Construcciones Arquitectónicas I
Microbiología
dc.subject.none.fl_str_mv UV-C light disinfection
Gram-negative pathogens
Healthcare-associated infections
topic UV-C light disinfection
Gram-negative pathogens
Healthcare-associated infections
description Objectives Health care–associated infections are a growing public health concern, with hospitals acting as reservoirs for pathogens. The rise in antimicrobial resistance worsens the issue. This study tested the effectiveness of the biodynamic lighting system (CHRONOlight) at varying ultraviolet (UV) light times and energy levels to reduce nosocomial gram-negative pathogens. Methods Eight clinical isolates, representing different gram-negative pathogens, were selected from the Andalusian reference laboratory. Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii ATCC were used as control strains. Different inoculums were exposed to type C UV light (UV-C) doses 2.0, 1.5, and 1.0 W/m2 (corresponding to an irradiation flux of 100%, 75%, and 50%, respectively, with spectral peaks at 270 nm) during 15, 30, 45, 60, and 300 seconds and type A UV light (UV-A) (380 nm) during 30 minutes. The bactericidal effect was considered when cellular viability decreased by ≥3-log10 colony forming unit/ml. UV-C light-surviving isolates were sequenced (Illumina MiSeq) and analyzed to identify mutations associated with UV-C light survival. The development of antibiotic resistance after UV-C exposure were also studied for the same surviving isolates. Results The efficacy of the CHRONOlight biodynamic lighting system varied by exposure time, UV dose, and bacterial species. E. coli isolates showed bactericidal effects within 15-30 seconds at 100% UV-C intensity, whereas Klebsiella pneumoniae required 30-45 seconds. P. aeruginosa and A. baumannii showed activity in 15-30 seconds, but clinical A. baumannii needed longer exposure, especially at lower UV-C intensities. No reduction in cell viability was seen with UV-A exposure. Gene mutations related to metabolism, stress response, motility, and virulence were found in survivors, and no cross-antibiotic resistance developed in surviving isolates. Conclusions CHRONOlight biodynamic lighting system is a novel broad-spectrum lighting prototype designed to effectively reduce the viability of gram-negative bacteria within short exposure times (30-300 seconds) through UV-C emission. This makes it a promising strategy for minimizing nosocomial infections in health care settings.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/178506
https://doi.org/10.1016/j.ijid.2025.108120
url https://hdl.handle.net/11441/178506
https://doi.org/10.1016/j.ijid.2025.108120
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv International Journal of Infectious Diseases, 161, 108120.
PID2020-117563RB-I00
https://www.ijidonline.com/article/S1201-9712(25)00342-X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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