Mobile origami immunosensors for the rapid detection of urinary tract infections

Urinary tract infections (UTI) have a high prevalence and can yield poor patient outcomes if they progress to urosepsis. Here we introduce mobile origami biosensors that detect UTIs caused by E. coli at the bedside in less than 7 minutes. The origami biosensors are made of a single piece of paper th...

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Detalhes bibliográficos
Autores: Adrover-Jaume, Cristina, Rojo Molinero, Estrella, Clemente, Antonio, Russell, Steven M, Arranz Izquierdo, Javier, Oliver, Antonio, de la Rica, Roberto
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/23338
Acesso em linha:https://hdl.handle.net/20.500.12105/23338
Access Level:acceso abierto
Palavra-chave:Inmunoensayo
Infecciones Urinarias
Humanos
Técnicas Biosensibles
Escherichia coli
Infecciones por Escherichia coli
Escherichia coli Infections
Urinary Tract Infections
Humans
Biosensing Techniques
Immunoassay
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oai_identifier_str oai:repisalud.isciii.es:20.500.12105/23338
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network_name_str España
repository_id_str
spelling Mobile origami immunosensors for the rapid detection of urinary tract infectionsAdrover-Jaume, CristinaRojo Molinero, EstrellaClemente, AntonioRussell, Steven MArranz Izquierdo, JavierOliver, Antoniode la Rica, RobertoInmunoensayoInfecciones UrinariasHumanosTécnicas BiosensiblesEscherichia coliInfecciones por Escherichia coliEscherichia coliEscherichia coli InfectionsUrinary Tract InfectionsHumansBiosensing TechniquesImmunoassayUrinary tract infections (UTI) have a high prevalence and can yield poor patient outcomes if they progress to urosepsis. Here we introduce mobile origami biosensors that detect UTIs caused by E. coli at the bedside in less than 7 minutes. The origami biosensors are made of a single piece of paper that contains antibody-decorated nanoparticles. When the urine sample contains E. coli, the biosensors generate colored spots on the paper strip. These are then quantified with a mobile app that calculates the pixel intensity in real time. The tests are highly specific and do not cross-react with other common uropathogens. Furthermore, the biosensors only yielded one false negative result when queried with a panel containing 57 urine samples from patients, which demonstrates that they have excellent sensitivity and specificity. This, along with the rapid assay time and smartphone-based detection, makes them useful for aiding in the diagnosis of UTIs at the point of care.Royal Society of Chemistry (RSC)20242024-09-1820212021-01-0720212021-01-07research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttps://hdl.handle.net/20.500.12105/23338reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)InglésengES CTQ2017-82432-Rand Not availableES CTQ2017-92226-EXP Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/233382026-06-12T12:43:37Z
dc.title.none.fl_str_mv Mobile origami immunosensors for the rapid detection of urinary tract infections
title Mobile origami immunosensors for the rapid detection of urinary tract infections
spellingShingle Mobile origami immunosensors for the rapid detection of urinary tract infections
Adrover-Jaume, Cristina
Inmunoensayo
Infecciones Urinarias
Humanos
Técnicas Biosensibles
Escherichia coli
Infecciones por Escherichia coli
Escherichia coli
Escherichia coli Infections
Urinary Tract Infections
Humans
Biosensing Techniques
Immunoassay
title_short Mobile origami immunosensors for the rapid detection of urinary tract infections
title_full Mobile origami immunosensors for the rapid detection of urinary tract infections
title_fullStr Mobile origami immunosensors for the rapid detection of urinary tract infections
title_full_unstemmed Mobile origami immunosensors for the rapid detection of urinary tract infections
title_sort Mobile origami immunosensors for the rapid detection of urinary tract infections
dc.creator.none.fl_str_mv Adrover-Jaume, Cristina
Rojo Molinero, Estrella
Clemente, Antonio
Russell, Steven M
Arranz Izquierdo, Javier
Oliver, Antonio
de la Rica, Roberto
author Adrover-Jaume, Cristina
author_facet Adrover-Jaume, Cristina
Rojo Molinero, Estrella
Clemente, Antonio
Russell, Steven M
Arranz Izquierdo, Javier
Oliver, Antonio
de la Rica, Roberto
author_role author
author2 Rojo Molinero, Estrella
Clemente, Antonio
Russell, Steven M
Arranz Izquierdo, Javier
Oliver, Antonio
de la Rica, Roberto
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Inmunoensayo
Infecciones Urinarias
Humanos
Técnicas Biosensibles
Escherichia coli
Infecciones por Escherichia coli
Escherichia coli
Escherichia coli Infections
Urinary Tract Infections
Humans
Biosensing Techniques
Immunoassay
topic Inmunoensayo
Infecciones Urinarias
Humanos
Técnicas Biosensibles
Escherichia coli
Infecciones por Escherichia coli
Escherichia coli
Escherichia coli Infections
Urinary Tract Infections
Humans
Biosensing Techniques
Immunoassay
description Urinary tract infections (UTI) have a high prevalence and can yield poor patient outcomes if they progress to urosepsis. Here we introduce mobile origami biosensors that detect UTIs caused by E. coli at the bedside in less than 7 minutes. The origami biosensors are made of a single piece of paper that contains antibody-decorated nanoparticles. When the urine sample contains E. coli, the biosensors generate colored spots on the paper strip. These are then quantified with a mobile app that calculates the pixel intensity in real time. The tests are highly specific and do not cross-react with other common uropathogens. Furthermore, the biosensors only yielded one false negative result when queried with a panel containing 57 urine samples from patients, which demonstrates that they have excellent sensitivity and specificity. This, along with the rapid assay time and smartphone-based detection, makes them useful for aiding in the diagnosis of UTIs at the point of care.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-07
2021
2021-01-07
2024
2024-09-18
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12105/23338
url https://hdl.handle.net/20.500.12105/23338
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv ES CTQ2017-82432-Rand Not available
ES CTQ2017-92226-EXP Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Chemistry (RSC)
publisher.none.fl_str_mv Royal Society of Chemistry (RSC)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405354858643456
score 15,81155

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