Therapeutic vaccination of woodchucks against chronic woodchuck hepatitis virus infection
BACKGROUND/AIMS: Therapeutic vaccination is a new approach to treat patients with chronic hepatitis B virus infection. We have used the woodchuck model to examine the efficacy and safety of this approach. METHODS: Seven woodchucks chronically infected with woodchuck hepatitis virus were immunized wi...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 1997 |
| País: | España |
| Institución: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/21597 |
| Acceso en línea: | https://hdl.handle.net/10171/21597 |
| Access Level: | acceso abierto |
| Palabra clave: | Antibodies Hepatitis Immune therapy Peptides T-cell help Vaccination Woodchuck Woodchuck hepatitis virus (WHV) Woodchuck hepatitis virus surface antigen (WHsAg) |
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Therapeutic vaccination of woodchucks against chronic woodchuck hepatitis virus infectionHervas-Stubbs, S. (Sandra)|||/items/8f56cb52-4465-4428-8acf-e50439c6be8fLasarte-Sagastibelza, J.J. (Juan José)|||/items/e366ad83-3db4-41ec-a9da-ed9159ba5c0cSarobe, P. (Pablo)|||/items/e6f6a7ac-cfe2-409e-ab14-057dea5fd160Prieto, J. (Jesús)|||/items/0d9c3dec-4a09-400d-8c83-23ece1096c71Cullen, J.M. (John M.)|||/items/f8eb5693-c98a-435d-80eb-7865a7801bb6Roggendorf, M. (Michael)|||/items/e648d4da-7ba1-40ea-99e8-0bca54c521e2Borras-Cuesta, F. (Francisco)|||/items/9f3719bd-4cf6-4e5b-b672-39179b54e8ccAntibodiesHepatitisImmune therapyPeptidesT-cell helpVaccinationWoodchuckWoodchuck hepatitis virus (WHV)Woodchuck hepatitis virus surface antigen (WHsAg)BACKGROUND/AIMS: Therapeutic vaccination is a new approach to treat patients with chronic hepatitis B virus infection. We have used the woodchuck model to examine the efficacy and safety of this approach. METHODS: Seven woodchucks chronically infected with woodchuck hepatitis virus were immunized with surface antigen from this virus, purified from plasma, in conjunction with a peptide named FIS (encompassing amino acids 106-118: FISEAIIHVLHSR from sperm whale myoglobin), which is recognized by T helper lymphocytes. As controls, two woodchucks chronically infected with woodchuck hepatitis virus were immunized: one with FIS only and the other with surface antigen only. RESULTS: Co-immunization with surface antigen and FIS, but not with FIS or surface antigen alone, induced anti-surface antibodies in 7/7 immunized woodchucks. In the two woodchucks in which the highest titer of anti-surface antibody was elicited, severe liver damage was observed: one died of fulminant hepatitis and the other became seriously ill with hepatic injury and had to be sacrificed. CONCLUSIONS: Co-immunization of chronically infected woodchucks with surface antigen and a peptide recognized by T helper cells produces a good anti-surface antibody response. However, this strategy needs to be optimized before its implementation in humans. Although our experiments are not strictly comparable to vaccination of chronically hepatitis B virus-infected patients with recombinant or plasma-derived vaccines, we believe that precautions should be taken to avoid the risk of severe liver injury when immunizing hepatitis B virus carriers.ElsevierDadun. Depósito Académico Digital Universidad de Navarra20122012-04-0419971997-01-0119971997-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/21597reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/215972026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Therapeutic vaccination of woodchucks against chronic woodchuck hepatitis virus infection |
| title |
Therapeutic vaccination of woodchucks against chronic woodchuck hepatitis virus infection |
| spellingShingle |
Therapeutic vaccination of woodchucks against chronic woodchuck hepatitis virus infection Hervas-Stubbs, S. (Sandra)|||/items/8f56cb52-4465-4428-8acf-e50439c6be8f Antibodies Hepatitis Immune therapy Peptides T-cell help Vaccination Woodchuck Woodchuck hepatitis virus (WHV) Woodchuck hepatitis virus surface antigen (WHsAg) |
| title_short |
Therapeutic vaccination of woodchucks against chronic woodchuck hepatitis virus infection |
| title_full |
Therapeutic vaccination of woodchucks against chronic woodchuck hepatitis virus infection |
| title_fullStr |
Therapeutic vaccination of woodchucks against chronic woodchuck hepatitis virus infection |
| title_full_unstemmed |
Therapeutic vaccination of woodchucks against chronic woodchuck hepatitis virus infection |
| title_sort |
Therapeutic vaccination of woodchucks against chronic woodchuck hepatitis virus infection |
| dc.creator.none.fl_str_mv |
Hervas-Stubbs, S. (Sandra)|||/items/8f56cb52-4465-4428-8acf-e50439c6be8f Lasarte-Sagastibelza, J.J. (Juan José)|||/items/e366ad83-3db4-41ec-a9da-ed9159ba5c0c Sarobe, P. (Pablo)|||/items/e6f6a7ac-cfe2-409e-ab14-057dea5fd160 Prieto, J. (Jesús)|||/items/0d9c3dec-4a09-400d-8c83-23ece1096c71 Cullen, J.M. (John M.)|||/items/f8eb5693-c98a-435d-80eb-7865a7801bb6 Roggendorf, M. (Michael)|||/items/e648d4da-7ba1-40ea-99e8-0bca54c521e2 Borras-Cuesta, F. (Francisco)|||/items/9f3719bd-4cf6-4e5b-b672-39179b54e8cc |
| author |
Hervas-Stubbs, S. (Sandra)|||/items/8f56cb52-4465-4428-8acf-e50439c6be8f |
| author_facet |
Hervas-Stubbs, S. (Sandra)|||/items/8f56cb52-4465-4428-8acf-e50439c6be8f Lasarte-Sagastibelza, J.J. (Juan José)|||/items/e366ad83-3db4-41ec-a9da-ed9159ba5c0c Sarobe, P. (Pablo)|||/items/e6f6a7ac-cfe2-409e-ab14-057dea5fd160 Prieto, J. (Jesús)|||/items/0d9c3dec-4a09-400d-8c83-23ece1096c71 Cullen, J.M. (John M.)|||/items/f8eb5693-c98a-435d-80eb-7865a7801bb6 Roggendorf, M. (Michael)|||/items/e648d4da-7ba1-40ea-99e8-0bca54c521e2 Borras-Cuesta, F. (Francisco)|||/items/9f3719bd-4cf6-4e5b-b672-39179b54e8cc |
| author_role |
author |
| author2 |
Lasarte-Sagastibelza, J.J. (Juan José)|||/items/e366ad83-3db4-41ec-a9da-ed9159ba5c0c Sarobe, P. (Pablo)|||/items/e6f6a7ac-cfe2-409e-ab14-057dea5fd160 Prieto, J. (Jesús)|||/items/0d9c3dec-4a09-400d-8c83-23ece1096c71 Cullen, J.M. (John M.)|||/items/f8eb5693-c98a-435d-80eb-7865a7801bb6 Roggendorf, M. (Michael)|||/items/e648d4da-7ba1-40ea-99e8-0bca54c521e2 Borras-Cuesta, F. (Francisco)|||/items/9f3719bd-4cf6-4e5b-b672-39179b54e8cc |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Antibodies Hepatitis Immune therapy Peptides T-cell help Vaccination Woodchuck Woodchuck hepatitis virus (WHV) Woodchuck hepatitis virus surface antigen (WHsAg) |
| topic |
Antibodies Hepatitis Immune therapy Peptides T-cell help Vaccination Woodchuck Woodchuck hepatitis virus (WHV) Woodchuck hepatitis virus surface antigen (WHsAg) |
| description |
BACKGROUND/AIMS: Therapeutic vaccination is a new approach to treat patients with chronic hepatitis B virus infection. We have used the woodchuck model to examine the efficacy and safety of this approach. METHODS: Seven woodchucks chronically infected with woodchuck hepatitis virus were immunized with surface antigen from this virus, purified from plasma, in conjunction with a peptide named FIS (encompassing amino acids 106-118: FISEAIIHVLHSR from sperm whale myoglobin), which is recognized by T helper lymphocytes. As controls, two woodchucks chronically infected with woodchuck hepatitis virus were immunized: one with FIS only and the other with surface antigen only. RESULTS: Co-immunization with surface antigen and FIS, but not with FIS or surface antigen alone, induced anti-surface antibodies in 7/7 immunized woodchucks. In the two woodchucks in which the highest titer of anti-surface antibody was elicited, severe liver damage was observed: one died of fulminant hepatitis and the other became seriously ill with hepatic injury and had to be sacrificed. CONCLUSIONS: Co-immunization of chronically infected woodchucks with surface antigen and a peptide recognized by T helper cells produces a good anti-surface antibody response. However, this strategy needs to be optimized before its implementation in humans. Although our experiments are not strictly comparable to vaccination of chronically hepatitis B virus-infected patients with recombinant or plasma-derived vaccines, we believe that precautions should be taken to avoid the risk of severe liver injury when immunizing hepatitis B virus carriers. |
| publishDate |
1997 |
| dc.date.none.fl_str_mv |
1997 1997-01-01 1997 1997-01-01 2012 2012-04-04 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/21597 |
| url |
https://hdl.handle.net/10171/21597 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
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Elsevier |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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1869405354825089024 |
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15.301603 |