Cardiogenesis impairment promoted by bisphenol A exposure is successfully counteracted by epigallocatechin gallate

[EN] Exposure to the emerging contaminant bisphenol A (BPA) is ubiquitous and associated with cardiovascular disorders. BPA effect as endocrine disruptor is widely known but other mechanisms underlying heart disease, such as epigenetic modifications, remain still unclear. A compound of green tea, ep...

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Detalles Bibliográficos
Autores: Lombó Alonso, Marta, González Rojo, Silvia, Fernández Díez, Cristina, Herráez Ortega, María Paz
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Universidad Rey Juan Carlos
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/18117
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S0269749118342738#sec7
https://hdl.handle.net/10612/18117
Access Level:acceso abierto
Palabra clave:Biología
Bisphenol A
Epigallocatechin Gallate
Cardiotoxicity
Histone acetylation
Cardiogenesis
2407 Biología Celular
3214 Toxicología
2401.07 Embriología Animal
2409.99 Otros (Epigenética)
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oai_identifier_str oai:buleria.unileon.es:10612/18117
network_acronym_str ES
network_name_str España
repository_id_str
spelling Cardiogenesis impairment promoted by bisphenol A exposure is successfully counteracted by epigallocatechin gallateLombó Alonso, MartaGonzález Rojo, SilviaFernández Díez, CristinaHerráez Ortega, María PazBiologíaBisphenol AEpigallocatechin GallateCardiotoxicityHistone acetylationCardiogenesis2407 Biología Celular3214 Toxicología2401.07 Embriología Animal2409.99 Otros (Epigenética)[EN] Exposure to the emerging contaminant bisphenol A (BPA) is ubiquitous and associated with cardiovascular disorders. BPA effect as endocrine disruptor is widely known but other mechanisms underlying heart disease, such as epigenetic modifications, remain still unclear. A compound of green tea, epigallocatechin gallate (EGCG), may act both as anti-estrogen and as inhibitor of some epigenetic enzymes. The aims of this study were to analyze the molecular processes related to BPA impairment of heart development and to prove the potential ability of EGCG to neutralize the toxic effects caused by BPA on cardiac health. Zebrafish embryos were exposed to 2000 and 4000 μg/L BPA and treated with 50 and 100 μM EGCG. Heart malformations were assessed at histological level and by confocal imaging. Expression of genes involved in cardiac development, estrogen receptors and epigenetic enzymes was analyzed by qPCR whereas epigenetic modifications were evaluated by whole mount immunostaining. BPA embryonic exposure led to changes in cardiac phenotype, induced an overexpression of hand2, a crucial factor for cardiomyocyte differentiation, increased the expression of estrogen receptor (esr2b), promoted an overexpression of a histone acetyltransferase (kat6a) and also caused an increase in histone acetylation, both mechanisms being able to act in sinergy. EGCG treatment neutralized all the molecular alterations caused by BPA, allowing the embryos to go on with a proper heart development. Both molecular mechanisms of BPA action (estrogenic and epigenetic) likely lying behind cardiogenesis impairment were successfully counteracted by EGCG treatmentSIThis work was funded by the Spanish Ministry of Economy and Competitiveness (Project AGL2014-53167-C3-3-R; PhD Grant BES-2015-071885).ElsevierBiologia CelularFacultad de Ciencias Biologicas y Ambientales2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttps://www.sciencedirect.com/science/article/pii/S0269749118342738#sec7https://hdl.handle.net/10612/18117reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad Rey Juan CarlosInglésinfo:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/AGL2014-53167-C3-3-Rinfo:eu-repo/grantAgreement/MINECO/Programa Estatal de Promoción del Talento y su Empleabilidad/BES- 2015-071885http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/181172026-06-24T12:43:27Z
dc.title.none.fl_str_mv Cardiogenesis impairment promoted by bisphenol A exposure is successfully counteracted by epigallocatechin gallate
title Cardiogenesis impairment promoted by bisphenol A exposure is successfully counteracted by epigallocatechin gallate
spellingShingle Cardiogenesis impairment promoted by bisphenol A exposure is successfully counteracted by epigallocatechin gallate
Lombó Alonso, Marta
Biología
Bisphenol A
Epigallocatechin Gallate
Cardiotoxicity
Histone acetylation
Cardiogenesis
2407 Biología Celular
3214 Toxicología
2401.07 Embriología Animal
2409.99 Otros (Epigenética)
title_short Cardiogenesis impairment promoted by bisphenol A exposure is successfully counteracted by epigallocatechin gallate
title_full Cardiogenesis impairment promoted by bisphenol A exposure is successfully counteracted by epigallocatechin gallate
title_fullStr Cardiogenesis impairment promoted by bisphenol A exposure is successfully counteracted by epigallocatechin gallate
title_full_unstemmed Cardiogenesis impairment promoted by bisphenol A exposure is successfully counteracted by epigallocatechin gallate
title_sort Cardiogenesis impairment promoted by bisphenol A exposure is successfully counteracted by epigallocatechin gallate
dc.creator.none.fl_str_mv Lombó Alonso, Marta
González Rojo, Silvia
Fernández Díez, Cristina
Herráez Ortega, María Paz
author Lombó Alonso, Marta
author_facet Lombó Alonso, Marta
González Rojo, Silvia
Fernández Díez, Cristina
Herráez Ortega, María Paz
author_role author
author2 González Rojo, Silvia
Fernández Díez, Cristina
Herráez Ortega, María Paz
author2_role author
author
author
dc.contributor.none.fl_str_mv Biologia Celular
Facultad de Ciencias Biologicas y Ambientales
dc.subject.none.fl_str_mv Biología
Bisphenol A
Epigallocatechin Gallate
Cardiotoxicity
Histone acetylation
Cardiogenesis
2407 Biología Celular
3214 Toxicología
2401.07 Embriología Animal
2409.99 Otros (Epigenética)
topic Biología
Bisphenol A
Epigallocatechin Gallate
Cardiotoxicity
Histone acetylation
Cardiogenesis
2407 Biología Celular
3214 Toxicología
2401.07 Embriología Animal
2409.99 Otros (Epigenética)
description [EN] Exposure to the emerging contaminant bisphenol A (BPA) is ubiquitous and associated with cardiovascular disorders. BPA effect as endocrine disruptor is widely known but other mechanisms underlying heart disease, such as epigenetic modifications, remain still unclear. A compound of green tea, epigallocatechin gallate (EGCG), may act both as anti-estrogen and as inhibitor of some epigenetic enzymes. The aims of this study were to analyze the molecular processes related to BPA impairment of heart development and to prove the potential ability of EGCG to neutralize the toxic effects caused by BPA on cardiac health. Zebrafish embryos were exposed to 2000 and 4000 μg/L BPA and treated with 50 and 100 μM EGCG. Heart malformations were assessed at histological level and by confocal imaging. Expression of genes involved in cardiac development, estrogen receptors and epigenetic enzymes was analyzed by qPCR whereas epigenetic modifications were evaluated by whole mount immunostaining. BPA embryonic exposure led to changes in cardiac phenotype, induced an overexpression of hand2, a crucial factor for cardiomyocyte differentiation, increased the expression of estrogen receptor (esr2b), promoted an overexpression of a histone acetyltransferase (kat6a) and also caused an increase in histone acetylation, both mechanisms being able to act in sinergy. EGCG treatment neutralized all the molecular alterations caused by BPA, allowing the embryos to go on with a proper heart development. Both molecular mechanisms of BPA action (estrogenic and epigenetic) likely lying behind cardiogenesis impairment were successfully counteracted by EGCG treatment
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0269749118342738#sec7
https://hdl.handle.net/10612/18117
url https://www.sciencedirect.com/science/article/pii/S0269749118342738#sec7
https://hdl.handle.net/10612/18117
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/AGL2014-53167-C3-3-R
info:eu-repo/grantAgreement/MINECO/Programa Estatal de Promoción del Talento y su Empleabilidad/BES- 2015-071885
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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