Effect of cadmium in the microalga Chlorella sorokiniana: A proteomic study

Cadmium is one of the most common heavy metals in contaminated aquatic environments and one of the most toxic contaminants for phytoplankton. Nevertheless, there are not enough studies focused on the effect of this metal in algae. Through a proteomic approach, this work shows how Cd can alter the gr...

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Detalhes bibliográficos
Autores: León Vaz, Antonio, Romero, Luis C., Gotor, Cecilia, León Bañares, Rosa María, Vigara Fernández, Javier
Formato: artículo
Fecha de publicación:2020
País:España
Recursos:Universidad de Huelva (UHU)
Repositorio:Arias Montano. Repositorio Institucional de la Universidad de Huelva
Idioma:inglés
OAI Identifier:oai:ariasmontano.uhu.es:10272/19142
Acesso em linha:http://hdl.handle.net/10272/19142
Access Level:acceso abierto
Palavra-chave:Microalgae
Chlorella sorokiniana
Heavy metal
Cadmium
Proteomics
Oxidative stress
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spelling Effect of cadmium in the microalga Chlorella sorokiniana: A proteomic studyLeón Vaz, AntonioRomero, Luis C.Gotor, CeciliaLeón Bañares, Rosa MaríaVigara Fernández, JavierMicroalgaeChlorella sorokinianaHeavy metalCadmiumProteomicsOxidative stressCadmium is one of the most common heavy metals in contaminated aquatic environments and one of the most toxic contaminants for phytoplankton. Nevertheless, there are not enough studies focused on the effect of this metal in algae. Through a proteomic approach, this work shows how Cd can alter the growth, cell morphology and metabolism of the microalga Chlorella sorokiniana. Using the sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS), we concluded that exposure of Chlorella sorokiniana to 250 μM Cd2+ for 40 h caused downregulation of different metabolic pathways, such as photosynthesis, oxidative phosphorylation, glycolysis, TCA cycle and ribosomal proteins biosynthesis. However, photorespiration, antioxidant enzymes, gluconeogenesis, starch catabolism, and biosynthesis of glutamate, cysteine, glycine and serine were upregulated, under the same conditions. Finally, exposure to Cd also led to changes in the metabolism of carotenoids and lipids. In addition, the high tolerance of Chlorella sorokiniana to Cd points to this microalga as a potential microorganism to be used in bioremediation processes.Elsevier20202020-09-0120202020-09-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10272/19142reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelvainstname:Universidad de Huelva (UHU)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:ariasmontano.uhu.es:10272/191422026-06-02T14:58:11Z
dc.title.none.fl_str_mv Effect of cadmium in the microalga Chlorella sorokiniana: A proteomic study
title Effect of cadmium in the microalga Chlorella sorokiniana: A proteomic study
spellingShingle Effect of cadmium in the microalga Chlorella sorokiniana: A proteomic study
León Vaz, Antonio
Microalgae
Chlorella sorokiniana
Heavy metal
Cadmium
Proteomics
Oxidative stress
title_short Effect of cadmium in the microalga Chlorella sorokiniana: A proteomic study
title_full Effect of cadmium in the microalga Chlorella sorokiniana: A proteomic study
title_fullStr Effect of cadmium in the microalga Chlorella sorokiniana: A proteomic study
title_full_unstemmed Effect of cadmium in the microalga Chlorella sorokiniana: A proteomic study
title_sort Effect of cadmium in the microalga Chlorella sorokiniana: A proteomic study
dc.creator.none.fl_str_mv León Vaz, Antonio
Romero, Luis C.
Gotor, Cecilia
León Bañares, Rosa María
Vigara Fernández, Javier
author León Vaz, Antonio
author_facet León Vaz, Antonio
Romero, Luis C.
Gotor, Cecilia
León Bañares, Rosa María
Vigara Fernández, Javier
author_role author
author2 Romero, Luis C.
Gotor, Cecilia
León Bañares, Rosa María
Vigara Fernández, Javier
author2_role author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Microalgae
Chlorella sorokiniana
Heavy metal
Cadmium
Proteomics
Oxidative stress
topic Microalgae
Chlorella sorokiniana
Heavy metal
Cadmium
Proteomics
Oxidative stress
description Cadmium is one of the most common heavy metals in contaminated aquatic environments and one of the most toxic contaminants for phytoplankton. Nevertheless, there are not enough studies focused on the effect of this metal in algae. Through a proteomic approach, this work shows how Cd can alter the growth, cell morphology and metabolism of the microalga Chlorella sorokiniana. Using the sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS), we concluded that exposure of Chlorella sorokiniana to 250 μM Cd2+ for 40 h caused downregulation of different metabolic pathways, such as photosynthesis, oxidative phosphorylation, glycolysis, TCA cycle and ribosomal proteins biosynthesis. However, photorespiration, antioxidant enzymes, gluconeogenesis, starch catabolism, and biosynthesis of glutamate, cysteine, glycine and serine were upregulated, under the same conditions. Finally, exposure to Cd also led to changes in the metabolism of carotenoids and lipids. In addition, the high tolerance of Chlorella sorokiniana to Cd points to this microalga as a potential microorganism to be used in bioremediation processes.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-09-01
2020
2020-09-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10272/19142
url http://hdl.handle.net/10272/19142
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelva
instname:Universidad de Huelva (UHU)
instname_str Universidad de Huelva (UHU)
reponame_str Arias Montano. Repositorio Institucional de la Universidad de Huelva
collection Arias Montano. Repositorio Institucional de la Universidad de Huelva
repository.name.fl_str_mv
repository.mail.fl_str_mv
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