Extracellular Vesicles from Probiotic and Beneficial Escherichia coli Strains Exert Multifaceted Protective Effects Against Rotavirus Infection in Intestinal Epithelial Cells

Rotavirus remains a major cause of severe acute gastroenteritisin infants worldwide. The suboptimal efficacy of current vaccines underscores the needfor alternative microbiome-based interventions, including postbiotics. Extracellularvesicles (EVs) from probiotic and commensal <em>E. coli</e...

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Autores: Cordero, Cecilia, Caballero Roman, Aitor, Martínez-Ruiz, Sergio, Olivo-Martinez, Yenifer, Baldomà Llavinés, Laura, Badía Palacín, Josefa
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/225945
Acceso en línea:https://hdl.handle.net/2445/225945
Access Level:acceso abierto
Palabra clave:Probiòtics
Microbiota intestinal
Gastroenteritis
Malalties intestinals
Probiotics
Gastrointestinal microbiome
Intestinal diseases
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spelling Extracellular Vesicles from Probiotic and Beneficial Escherichia coli Strains Exert Multifaceted Protective Effects Against Rotavirus Infection in Intestinal Epithelial CellsCordero, CeciliaCaballero Roman, AitorMartínez-Ruiz, SergioOlivo-Martinez, YeniferBaldomà Llavinés, LauraBadía Palacín, JosefaProbiòticsMicrobiota intestinalGastroenteritisMalalties intestinalsProbioticsGastrointestinal microbiomeGastroenteritisIntestinal diseasesRotavirus remains a major cause of severe acute gastroenteritisin infants worldwide. The suboptimal efficacy of current vaccines underscores the needfor alternative microbiome-based interventions, including postbiotics. Extracellularvesicles (EVs) from probiotic and commensal <em>E. coli</em> strains have been shown to mitigatediarrhea and enhance immune responses in a suckling-rat model of rotavirus infection.Here, we investigate the regulatory mechanisms activated by EVs in rotavirus-infectedenterocytes. <strong>Methods: </strong>Polarized Caco-2 monolayers were used as a model of matureenterocytes. Cells were pre-incubated with EVs from the probiotic <em>E. coli</em> Nissle 1917 (EcN)or the commensal EcoR12 strain before rotavirus infection. Intracellular Ca<sup>2+</sup>concentration, ROS levels, and the expression of immune- and barrier-related genes andproteins were assessed at multiple time points post-infection. <strong>Results:</strong> EVs from bothstrains exerted broad protective effects against rotavirus-induced cellular dysregulation,with several responses being strain-specific. EVs interfered with viral replication bycounteracting host cellular processes essential for rotavirus propagation. Specifically, EVtreatment significantly reduced rotavirus-induced intracellular Ca<sup>2+</sup> mobilization, ROSproduction, and COX-2 expression. In addition, both EV types reduced virus-inducedmucin secretion and preserved tight junction organization, thereby limiting viral accessto basolateral coreceptors. Additionally, EVs enhanced innate antiviral defenses viadistinct, strain-dependent pathways: EcN EVs amplified IL-8-mediated responses,whereas EcoR12 EVs preserved the expression of interferon-related signaling genes.<strong>Conclusions:</strong> EVs from EcN and EcoR12 act through multiple complementarymechanisms to restrict rotavirus replication, spread, and immune evasion. These findingssupport their potential as effective postbiotic candidates for preventing or treatingrotavirus infection.MDPI2026202620262026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion27 p.application/pdfhttps://hdl.handle.net/2445/225945Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/pharmaceutics18010120Pharmaceutics, 2026, vol. 18, num.1https://doi.org/10.3390/pharmaceutics18010120cc-by (c) Cordero, C. et al., 2026http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2259452026-05-29T05:05:01Z
dc.title.none.fl_str_mv Extracellular Vesicles from Probiotic and Beneficial Escherichia coli Strains Exert Multifaceted Protective Effects Against Rotavirus Infection in Intestinal Epithelial Cells
title Extracellular Vesicles from Probiotic and Beneficial Escherichia coli Strains Exert Multifaceted Protective Effects Against Rotavirus Infection in Intestinal Epithelial Cells
spellingShingle Extracellular Vesicles from Probiotic and Beneficial Escherichia coli Strains Exert Multifaceted Protective Effects Against Rotavirus Infection in Intestinal Epithelial Cells
Cordero, Cecilia
Probiòtics
Microbiota intestinal
Gastroenteritis
Malalties intestinals
Probiotics
Gastrointestinal microbiome
Gastroenteritis
Intestinal diseases
title_short Extracellular Vesicles from Probiotic and Beneficial Escherichia coli Strains Exert Multifaceted Protective Effects Against Rotavirus Infection in Intestinal Epithelial Cells
title_full Extracellular Vesicles from Probiotic and Beneficial Escherichia coli Strains Exert Multifaceted Protective Effects Against Rotavirus Infection in Intestinal Epithelial Cells
title_fullStr Extracellular Vesicles from Probiotic and Beneficial Escherichia coli Strains Exert Multifaceted Protective Effects Against Rotavirus Infection in Intestinal Epithelial Cells
title_full_unstemmed Extracellular Vesicles from Probiotic and Beneficial Escherichia coli Strains Exert Multifaceted Protective Effects Against Rotavirus Infection in Intestinal Epithelial Cells
title_sort Extracellular Vesicles from Probiotic and Beneficial Escherichia coli Strains Exert Multifaceted Protective Effects Against Rotavirus Infection in Intestinal Epithelial Cells
dc.creator.none.fl_str_mv Cordero, Cecilia
Caballero Roman, Aitor
Martínez-Ruiz, Sergio
Olivo-Martinez, Yenifer
Baldomà Llavinés, Laura
Badía Palacín, Josefa
author Cordero, Cecilia
author_facet Cordero, Cecilia
Caballero Roman, Aitor
Martínez-Ruiz, Sergio
Olivo-Martinez, Yenifer
Baldomà Llavinés, Laura
Badía Palacín, Josefa
author_role author
author2 Caballero Roman, Aitor
Martínez-Ruiz, Sergio
Olivo-Martinez, Yenifer
Baldomà Llavinés, Laura
Badía Palacín, Josefa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Probiòtics
Microbiota intestinal
Gastroenteritis
Malalties intestinals
Probiotics
Gastrointestinal microbiome
Gastroenteritis
Intestinal diseases
topic Probiòtics
Microbiota intestinal
Gastroenteritis
Malalties intestinals
Probiotics
Gastrointestinal microbiome
Gastroenteritis
Intestinal diseases
description Rotavirus remains a major cause of severe acute gastroenteritisin infants worldwide. The suboptimal efficacy of current vaccines underscores the needfor alternative microbiome-based interventions, including postbiotics. Extracellularvesicles (EVs) from probiotic and commensal <em>E. coli</em> strains have been shown to mitigatediarrhea and enhance immune responses in a suckling-rat model of rotavirus infection.Here, we investigate the regulatory mechanisms activated by EVs in rotavirus-infectedenterocytes. <strong>Methods: </strong>Polarized Caco-2 monolayers were used as a model of matureenterocytes. Cells were pre-incubated with EVs from the probiotic <em>E. coli</em> Nissle 1917 (EcN)or the commensal EcoR12 strain before rotavirus infection. Intracellular Ca<sup>2+</sup>concentration, ROS levels, and the expression of immune- and barrier-related genes andproteins were assessed at multiple time points post-infection. <strong>Results:</strong> EVs from bothstrains exerted broad protective effects against rotavirus-induced cellular dysregulation,with several responses being strain-specific. EVs interfered with viral replication bycounteracting host cellular processes essential for rotavirus propagation. Specifically, EVtreatment significantly reduced rotavirus-induced intracellular Ca<sup>2+</sup> mobilization, ROSproduction, and COX-2 expression. In addition, both EV types reduced virus-inducedmucin secretion and preserved tight junction organization, thereby limiting viral accessto basolateral coreceptors. Additionally, EVs enhanced innate antiviral defenses viadistinct, strain-dependent pathways: EcN EVs amplified IL-8-mediated responses,whereas EcoR12 EVs preserved the expression of interferon-related signaling genes.<strong>Conclusions:</strong> EVs from EcN and EcoR12 act through multiple complementarymechanisms to restrict rotavirus replication, spread, and immune evasion. These findingssupport their potential as effective postbiotic candidates for preventing or treatingrotavirus infection.
publishDate 2026
dc.date.none.fl_str_mv 2026
2026
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/225945
url https://hdl.handle.net/2445/225945
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/pharmaceutics18010120
Pharmaceutics, 2026, vol. 18, num.1
https://doi.org/10.3390/pharmaceutics18010120
dc.rights.none.fl_str_mv cc-by (c) Cordero, C. et al., 2026
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Cordero, C. et al., 2026
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 27 p.
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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