A bioguided identification of the active compounds that contribute to the antiproliferative/cytotoxic effects of rosemary extract on colon cancer cells

et al.

Bibliographic Details
Authors: Borrás-Linares, Isabel, Lozano-Sánchez, Jesús, Cifuentes, Alejandro, Micol, Vicente, Segura-Carretero, Antonio
Format: article
Status:Versión aceptada para publicación
Publication Date:2015
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::9e8bc3be56ed4c823e11fdb2fee6dba7
Online Access:http://hdl.handle.net/10261/149690
Access Level:Open access
Keyword:Terpenoids
Colon cancer
HPLC-ESI-QTOF-MS
Antiproliferative activity
Cytotoxicity
Rosemary
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spelling A bioguided identification of the active compounds that contribute to the antiproliferative/cytotoxic effects of rosemary extract on colon cancer cellsBorrás-Linares, IsabelLozano-Sánchez, JesúsCifuentes, AlejandroMicol, VicenteSegura-Carretero, AntonioTerpenoidsColon cancerHPLC-ESI-QTOF-MSAntiproliferative activityCytotoxicityRosemaryet al.Rosemary extracts have exhibited potential cytostatic or cytotoxic effects in several cancer cell models but their bioactive compounds are yet to be discovered. In this work, the anticancer activity of a rosemary-leaf extract and its fractions were assayed to identify the phenolic compounds responsible for their antiproliferative/cytotoxic effects on a panel of human colon cancer cell lines. Bioguided fractionation of the rosemary-leaf extract was achieved by semi-preparative chromatography. The rosemary extract and the compounds in the fractions were characterized and quantified by HPLC-ESI-QTOF-MS. Cellular viability in the presence of these fractions and the whole extract was determined after 24 or 48¿h incubations by using an MTT assay. Fractions containing diterpenes or triterpenes were the most active but not as much as the whole extract. In conclusion, carnosic acid, carnosol, 12-methoxycarnosic acid, taxodione, hinokione and betulinic acid were the putative candidates that contributed to the observed antiproliferative activity of rosemary in human colon cancer cells. Whether the effects of the extract and fractions are only cytostatic or cytotoxic needs to be elucidated. Nevertheless, the comparative antiproliferative study on the fractions and whole extract revealed potential synergistic effects between several components in the extract that may deserve further attention.This work was supported by projects AGL2011-29857-C03-02 and AGL2011-29857-C03-03 (Spanish Ministry of Science and Innovation), in addition to P10-FQM-6563 and P11-CTS-7625 (Andalusian Regional Government Council of Innovation and Science), PROMETEO/2012/007 and ACOMP/2013/093 (Generalitat Valenciana), and CIBER (CB12/03/30038, Fisiopatología de la Obesidad y la Nutrición, CIBERobn, Instituto de Salud Carlos III). The authors are grateful to the Spanish Ministry of Science and Innovation for grants FPU (AP2007-03246) and FPI (BES-2009-028128), and the Spanish Ministry of Economy and Competitiveness (MINECO) for the projects BFU2014-52433-C3-1-R, BFU2014-52433-C3-2-R and in association with the European Social Fund (FSE) for the contract PTQ-13-06429.Peer ReviewedElsevierJunta de AndalucíaEuropean CommissionMinisterio de Ciencia e Innovación (España)Ministerio de Economía y Competitividad (España)Generalitat ValencianaInstituto de Salud Carlos IIIConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720152017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/149690reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014-52433-C3-2-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014-52433-C3-1-Rhttps://doi.org/10.1016/j.fct.2015.03.013Síinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::9e8bc3be56ed4c823e11fdb2fee6dba72026-05-22T06:33:51Z
dc.title.none.fl_str_mv A bioguided identification of the active compounds that contribute to the antiproliferative/cytotoxic effects of rosemary extract on colon cancer cells
title A bioguided identification of the active compounds that contribute to the antiproliferative/cytotoxic effects of rosemary extract on colon cancer cells
spellingShingle A bioguided identification of the active compounds that contribute to the antiproliferative/cytotoxic effects of rosemary extract on colon cancer cells
Borrás-Linares, Isabel
Terpenoids
Colon cancer
HPLC-ESI-QTOF-MS
Antiproliferative activity
Cytotoxicity
Rosemary
title_short A bioguided identification of the active compounds that contribute to the antiproliferative/cytotoxic effects of rosemary extract on colon cancer cells
title_full A bioguided identification of the active compounds that contribute to the antiproliferative/cytotoxic effects of rosemary extract on colon cancer cells
title_fullStr A bioguided identification of the active compounds that contribute to the antiproliferative/cytotoxic effects of rosemary extract on colon cancer cells
title_full_unstemmed A bioguided identification of the active compounds that contribute to the antiproliferative/cytotoxic effects of rosemary extract on colon cancer cells
title_sort A bioguided identification of the active compounds that contribute to the antiproliferative/cytotoxic effects of rosemary extract on colon cancer cells
dc.creator.none.fl_str_mv Borrás-Linares, Isabel
Lozano-Sánchez, Jesús
Cifuentes, Alejandro
Micol, Vicente
Segura-Carretero, Antonio
author Borrás-Linares, Isabel
author_facet Borrás-Linares, Isabel
Lozano-Sánchez, Jesús
Cifuentes, Alejandro
Micol, Vicente
Segura-Carretero, Antonio
author_role author
author2 Lozano-Sánchez, Jesús
Cifuentes, Alejandro
Micol, Vicente
Segura-Carretero, Antonio
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Junta de Andalucía
European Commission
Ministerio de Ciencia e Innovación (España)
Ministerio de Economía y Competitividad (España)
Generalitat Valenciana
Instituto de Salud Carlos III
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Terpenoids
Colon cancer
HPLC-ESI-QTOF-MS
Antiproliferative activity
Cytotoxicity
Rosemary
topic Terpenoids
Colon cancer
HPLC-ESI-QTOF-MS
Antiproliferative activity
Cytotoxicity
Rosemary
description et al.
publishDate 2015
dc.date.none.fl_str_mv 2015
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/149690
url http://hdl.handle.net/10261/149690
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014-52433-C3-2-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014-52433-C3-1-R
https://doi.org/10.1016/j.fct.2015.03.013
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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