Ultrasound-assisted Co-extraction and characterization of saponin-rich Co-extracts from fenugreek seeds (Trigonella foenum-graecum L.) and quinoa husk (Chenopodium quinoa Willd.) and their hydrolysates
The co-extraction of steroidal and triterpenoid saponins, followed by hydrolysis to produce sapogenin-rich extracts, offers an efficient strategy to enhance the multi-bioactive potential of extracts. The resulting chemical composition modifications, including the presence of other co-extracted or co...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/117966 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/117966 |
| Access Level: | acceso abierto |
| Palabra clave: | 636.09 Co-extraction Saponins Sapogenins Phenolic compounds Microwave-assisted acid hydrolysis Ultrasound-assisted extraction Ciencias Biomédicas 24 Ciencias de la Vida |
| Sumario: | The co-extraction of steroidal and triterpenoid saponins, followed by hydrolysis to produce sapogenin-rich extracts, offers an efficient strategy to enhance the multi-bioactive potential of extracts. The resulting chemical composition modifications, including the presence of other co-extracted or co-hydrolyzed compounds, must also be considered. In this study, ultrasound-assisted co-extraction of fenugreek seeds and quinoa husk using methanol and 50% aqueous methanol was conducted to obtain extracts and co-extracts with varying proportions of steroidal and triterpenoid saponins, in ratios of 0:100, 25:75, 50:50, 75:25, and 100:0, respectively. Aqueous methanol produced the highest extraction yields (15–25%) and saponin contents (40–70%) in the extracts and co-extracts. The conversion by microwave-assisted acid hydrolysis of saponins to sapogenins was more efficient for triterpenoid saponins, leading to a higher triterpenoid sapogenin content in all co-extracts, accounting for over 70% of the total sapogenins. The co-extracts also contained other bioactive compounds, such as phenolic compounds (3–4 g/100 g), with their content being favored by the presence of fenugreek. Hydrolysis altered the overall composition of the saponin-rich co-extracts, enriching the resulting hydrolyzed products in both types of sapogenins, along with fatty acids, carbonyls, non-protein nitrogen compounds and organic acids, while reducing monoglycerides, removing carbohydrates and significantly modifying the phenolic profile by eliminating many original phenolic compounds and generating new ones. Therefore, co-extraction and post-extraction hydrolysis of fenugreek and quinoa allowed the combination of triterpenoid and steroidal saponins or sapogenins into single products, along with other specific compounds from both sources. |
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