Usefulness of Housekeeping Genes for the Diagnosis of Helicobacter pylori Infection, Strain Discrimination and Detection of Multiple Infection
<b>Background:</b> <i>Helicobacter pylori</i> infects human stomachs of over half the world's population, evades the immune response and establishes a chronic infection. Although most people remains asymptomatic, duodenal and gastric ulcers, MALT lymphoma and progression...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/168584 |
| Acceso en línea: | https://hdl.handle.net/2445/168584 |
| Access Level: | acceso abierto |
| Palabra clave: | Helicobacteri pilòric Genètica bacteriana Infeccions Helicobacter pylori Bacterial genetics Infections |
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Usefulness of Housekeeping Genes for the Diagnosis of Helicobacter pylori Infection, Strain Discrimination and Detection of Multiple InfectionPalau de Miguel, MontserratKulmann, MarcosRamírez-Lázaro, María JoséLario, SergioQuilez, María ElisaCampo Fernández de los Rios, RafaelPiqué i Clusella, NúriaCalvet, XavierMiñana i Galbis, DavidHelicobacteri pilòricGenètica bacterianaInfeccionsHelicobacter pyloriBacterial geneticsInfections<b>Background:</b> <i>Helicobacter pylori</i> infects human stomachs of over half the world's population, evades the immune response and establishes a chronic infection. Although most people remains asymptomatic, duodenal and gastric ulcers, MALT lymphoma and progression to gastric cancer could be developed. Several virulence factors such as flagella, lipopolysaccharide, adhesins and especially the vacuolating cytotoxin VacA and the oncoprotein CagA have been described for <i>H. pylori</i>. Despite the extensive published data on <i>H. pylori</i>, more research is needed to determine new virulence markers, the exact mode of transmission or the role of multiple infection. <b>Materials and Methods:</b> Amplification and sequencing of six housekeeping genes (<i>amiA</i>, <i>cgt</i>, <i>cpn60</i>, <i>cpn70</i>, <i>dnaJ</i>, and <i>luxS</i>) related to <i>H. pylori</i> pathogenesis have been performed in order to evaluate their usefulness for the specific detection of <i>H. pylori</i>, the genetic discrimination at strain level and the detection of multiple infection. A total of 52 <i>H. pylori</i> clones, isolated from 14 gastric biopsies from 11 patients, were analyzed for this purpose. <b>Results:</b> All genes were specifically amplified for <i>H. pylori</i> and all clones isolated from different patients were discriminated, with gene distances ranged from 0.9 to 7.8%. Although most clones isolated from the same patient showed identical gene sequences, an event of multiple infection was detected in all the genes and microevolution events were showed for <i>amiA</i> and <i>cpn60</i> genes. <b>Conclusions:</b> These results suggested that housekeeping genes could be useful for <i>H. pylori</i> detection and to elucidate the mode of transmission and the relevance of the multiple infection.John Wiley & Sons2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/168584Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1111/hel.12304Helicobacter, 2016, vol. 21, p. 481-487https://doi.org/10.1111/hel.12304(c) John Wiley & Sons, 2016info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1685842026-05-27T06:46:51Z |
| dc.title.none.fl_str_mv |
Usefulness of Housekeeping Genes for the Diagnosis of Helicobacter pylori Infection, Strain Discrimination and Detection of Multiple Infection |
| title |
Usefulness of Housekeeping Genes for the Diagnosis of Helicobacter pylori Infection, Strain Discrimination and Detection of Multiple Infection |
| spellingShingle |
Usefulness of Housekeeping Genes for the Diagnosis of Helicobacter pylori Infection, Strain Discrimination and Detection of Multiple Infection Palau de Miguel, Montserrat Helicobacteri pilòric Genètica bacteriana Infeccions Helicobacter pylori Bacterial genetics Infections |
| title_short |
Usefulness of Housekeeping Genes for the Diagnosis of Helicobacter pylori Infection, Strain Discrimination and Detection of Multiple Infection |
| title_full |
Usefulness of Housekeeping Genes for the Diagnosis of Helicobacter pylori Infection, Strain Discrimination and Detection of Multiple Infection |
| title_fullStr |
Usefulness of Housekeeping Genes for the Diagnosis of Helicobacter pylori Infection, Strain Discrimination and Detection of Multiple Infection |
| title_full_unstemmed |
Usefulness of Housekeeping Genes for the Diagnosis of Helicobacter pylori Infection, Strain Discrimination and Detection of Multiple Infection |
| title_sort |
Usefulness of Housekeeping Genes for the Diagnosis of Helicobacter pylori Infection, Strain Discrimination and Detection of Multiple Infection |
| dc.creator.none.fl_str_mv |
Palau de Miguel, Montserrat Kulmann, Marcos Ramírez-Lázaro, María José Lario, Sergio Quilez, María Elisa Campo Fernández de los Rios, Rafael Piqué i Clusella, Núria Calvet, Xavier Miñana i Galbis, David |
| author |
Palau de Miguel, Montserrat |
| author_facet |
Palau de Miguel, Montserrat Kulmann, Marcos Ramírez-Lázaro, María José Lario, Sergio Quilez, María Elisa Campo Fernández de los Rios, Rafael Piqué i Clusella, Núria Calvet, Xavier Miñana i Galbis, David |
| author_role |
author |
| author2 |
Kulmann, Marcos Ramírez-Lázaro, María José Lario, Sergio Quilez, María Elisa Campo Fernández de los Rios, Rafael Piqué i Clusella, Núria Calvet, Xavier Miñana i Galbis, David |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
Helicobacteri pilòric Genètica bacteriana Infeccions Helicobacter pylori Bacterial genetics Infections |
| topic |
Helicobacteri pilòric Genètica bacteriana Infeccions Helicobacter pylori Bacterial genetics Infections |
| description |
<b>Background:</b> <i>Helicobacter pylori</i> infects human stomachs of over half the world's population, evades the immune response and establishes a chronic infection. Although most people remains asymptomatic, duodenal and gastric ulcers, MALT lymphoma and progression to gastric cancer could be developed. Several virulence factors such as flagella, lipopolysaccharide, adhesins and especially the vacuolating cytotoxin VacA and the oncoprotein CagA have been described for <i>H. pylori</i>. Despite the extensive published data on <i>H. pylori</i>, more research is needed to determine new virulence markers, the exact mode of transmission or the role of multiple infection. <b>Materials and Methods:</b> Amplification and sequencing of six housekeeping genes (<i>amiA</i>, <i>cgt</i>, <i>cpn60</i>, <i>cpn70</i>, <i>dnaJ</i>, and <i>luxS</i>) related to <i>H. pylori</i> pathogenesis have been performed in order to evaluate their usefulness for the specific detection of <i>H. pylori</i>, the genetic discrimination at strain level and the detection of multiple infection. A total of 52 <i>H. pylori</i> clones, isolated from 14 gastric biopsies from 11 patients, were analyzed for this purpose. <b>Results:</b> All genes were specifically amplified for <i>H. pylori</i> and all clones isolated from different patients were discriminated, with gene distances ranged from 0.9 to 7.8%. Although most clones isolated from the same patient showed identical gene sequences, an event of multiple infection was detected in all the genes and microevolution events were showed for <i>amiA</i> and <i>cpn60</i> genes. <b>Conclusions:</b> These results suggested that housekeeping genes could be useful for <i>H. pylori</i> detection and to elucidate the mode of transmission and the relevance of the multiple infection. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion |
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article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/168584 |
| url |
https://hdl.handle.net/2445/168584 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Versió postprint del document publicat a: https://doi.org/10.1111/hel.12304 Helicobacter, 2016, vol. 21, p. 481-487 https://doi.org/10.1111/hel.12304 |
| dc.rights.none.fl_str_mv |
(c) John Wiley & Sons, 2016 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
(c) John Wiley & Sons, 2016 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
John Wiley & Sons |
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John Wiley & Sons |
| dc.source.none.fl_str_mv |
Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) reponame:Dipòsit Digital de la UB instname:Universidad de Barcelona |
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Universidad de Barcelona |
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Dipòsit Digital de la UB |
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Dipòsit Digital de la UB |
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